草地学报

›› 2011, Vol. 19 ›› Issue (2): 283-287.DOI: 10.11733/j.issn.1007-0435.2011.02.019

• 研究论文 • 上一篇    下一篇

长白山单花橐吾茎尖离体培养研究

庄倩倩1, 李文鑫2, 顾德峰1, 陈少鹏1, 董然1   

  1. 1. 吉林农业大学园艺学院, 吉林, 长春, 130118;
    2. 吉林省黄泥河林业局, 吉林, 敦化, 133704
  • 收稿日期:2010-09-26 修回日期:2011-02-17 出版日期:2011-04-15 发布日期:2011-04-15
  • 通讯作者: 董然,E-mail:Dongr999@163.com
  • 作者简介:庄倩倩(1984- ),女,吉林省临江市人,硕士研究生,研究方向为野生植物引种驯化,E-mail:zhuangqian_0156@sina.com
  • 基金资助:
    吉林省科学技术厅-长白山特种经济植物保存及配套关键技术研究(20100259)资助

In Vitro Tip Micropropagation of Ligularia jamesii from Changbai Mountain

ZHUANG Qian-qian1, LI Wen-xin2, GU De-feng1, CHEN Shao-peng1, DONG Ran1   

  1. 1. College of Horticulture, Jilin Agricultural University, Changchun, Jilin Province 130118, China;
    2. Huang Nihe forestry bureau of Jilin Province, Dunhua, Jilin Province 133704, China
  • Received:2010-09-26 Revised:2011-02-17 Online:2011-04-15 Published:2011-04-15

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摘要： 以单花橐吾(Ligularia jamesii)茎尖作为外植体进行幼芽诱导,探索单花橐吾的离体快繁途径。结果表明:在MS+6-BA(3.0 mg·L-1)+NAA(0.3 mg·L-1)+蔗糖(35 g·L-1)培养基上的幼芽诱导效果最好,诱导率高达90.1%;增殖培养时在MS+6-BA(2.5 mg·L-1)+IBA(0.1 mg·L-1)培养基上的平均增殖系数4.9,幼芽生长健壮,可继代培养6次以上;生根培养采用1/2MS+NAA(0.5 mg·L-1),生根率达95%,生根白色粗壮,且数量较多。

关键词: 单花橐吾, 茎尖, 快繁

Abstract: Stem tips of Ligularia jamesii were used as explants to induce plumule proliferation.Results showed that the best combinations inducing plumules are MS medium supplemented with 6-BA(3.0 mg·L-1) and NAA(0.3 mg·L-1).During the initial culture,the induction rate reached 90.1%.MS medium supplemented with 6-BA(2.5 mg·L-1) and IBA(0.1 mg·L-1) is used for propagation subculture,while propagation coefficient is 4.9 and the plantlets grew vigorously.For rooting culture,1/2MS medium with NAA(0.5 mg·L-1) gave best results with white roots well developed and 95% rooting rate.

Key words: Ligularia jamesii, Stem tip, Micropropagation

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