低磷胁迫对崖州硬皮豆生长及酸性磷酸酶活性的影响

doi:10.11733/j.issn.1007-0435.2021.07.011

草地学报 ›› 2021, Vol. 29 ›› Issue (7): 1462-1468.DOI: 10.11733/j.issn.1007-0435.2021.07.011

低磷胁迫对崖州硬皮豆生长及酸性磷酸酶活性的影响

黄杰^1,2, 张郎织², 邢玉芬¹, 邹晓燕², 刘国道¹, 虞道耿¹, 陈志坚^1,2

1. 中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室, 海南海口 571101;
2. 海南大学热带作物学院, 海南海口 570228

收稿日期:2021-02-23 修回日期:2021-03-25 出版日期:2021-07-15 发布日期:2021-07-31
通讯作者: 陈志坚,E-mail:jchen@scau.edu.cn;虞道耿,E-mail:geng0209@126.com
作者简介:黄杰(1993-),男,河南信阳人,硕士研究生,主要从事热带牧草植物营养胁迫研究,E-mail:260633452@qq.com
基金资助:
财政部和农业农村部：国家现代农业产业技术体系；滇桂黔石漠化地区特色作物产业发展关键技术集成示范项目（SMH2019-2021）；海南省农业农村厅物种资源保护项目资助

Effects of Low Phosphorus Stress on the Growth and Acid Phosphatase Activity of Macrotyloma uniflorum (Lam.) Verdc. Yazhou

HUANG Jie^1,2, ZHANG Lang-zhi², XING Yu-fen¹, ZOU Xiao-yan², LIU Guo-dao¹, YU Dao-geng¹, CHEN Zhi-jian^1,2

1. Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan Province 571101, China;
2. College of Tropical Crops, Hainan University, Haikou, Hainan Province 570228, China

Received:2021-02-23 Revised:2021-03-25 Online:2021-07-15 Published:2021-07-31

摘要/Abstract

摘要： 本试验为探究硬皮豆（Macrotyloma uniflorum）对低磷胁迫的响应机制，以‘崖州硬皮豆’为材料，采用水培方法，分析了不同磷浓度（5 μM，100 μM和250 μM KH₂PO₄）处理对硬皮豆生长和生理特性的影响。结果表明：与100 μM和250 μM磷处理相比，5 μM低磷处理抑制了硬皮豆株高、复叶数、叶面积、总根长，根表面积和根体积以及植株干重。同时，5 μM磷处理显著降低了地上部和根部磷含量、降低了叶片和根系可溶性磷含量和细胞壁磷含量；与100 μM和250 μM磷处理相比，5 μM磷处理增加了叶片和根系总酸性磷酸酶（Acid phosphatase，ACP）活性和细胞壁ACP活性；5 μM磷处理下叶片和根系总ACP活性是250 μM磷条件下的1.5~1.6倍，而叶片和根系细胞壁ACP活性是250 μM磷条件下的1.2~1.3倍。因此，提高酸性磷酸酶活性可能是硬皮豆响应低磷胁迫的重要机制。

关键词: 硬皮豆, 低磷胁迫, 酸性磷酸酶, 磷含量

Abstract: Low phosphorus (P) stress is one of the constraints on crop growth and yield. ‘Macrotyloma uniflorum (Lam.) Verdc. Yazhou’ is a high-quality forage for livestock,but its response mechanisms on low P availability are largely unknown. In this study,in order to investigate the effects of low P stress on the growth and physiological characteristics of M.uniflorum,plant growth,P concentration and acid phosphatase (ACP) activity of M.uniflorum under different P treatments (5 μM,100 μM and 250 μM KH₂PO₄) were analyzed in hydroponic conditions. Results showed that plant height,number of trifoliate leaf,leaf area,total root length,root surface area,root volume and, plant dry weight of M. uniflorum were significantly inhibited by 5 μM P treatment compared to 100 μM and 250 μM P treatments. In addition,5 μM P treatment significantly reduced P content,soluble P content and cell wall P content. However,increasing in the total ACP and cell wall ACP activities in leaves and roots was observed under 5 μM P condition compared to 100 μM and 250 μM P treatments. The total ACP activities in leaves and roots of M. uniflorum under 5 μM P treatment was 1.5~1.6 times higher than those under 250 μM P treatment,respectively. Furthermore,cell wall ACP activity in leaves and roots was 1.2~1.3 times higher than those under 250 μM P treatment,respectively. The results suggest that M. uniflorum could induce more acid phosphatase in response to P deficiency,thereby increasing P utilization.

Key words: Macrotyloma uniflorum, Low P stress, Acid phosphatase, P content

中图分类号:

S153

黄杰, 张郎织, 邢玉芬, 邹晓燕, 刘国道, 虞道耿, 陈志坚. 低磷胁迫对崖州硬皮豆生长及酸性磷酸酶活性的影响[J]. 草地学报, 2021, 29(7): 1462-1468.

HUANG Jie, ZHANG Lang-zhi, XING Yu-fen, ZOU Xiao-yan, LIU Guo-dao, YU Dao-geng, CHEN Zhi-jian. Effects of Low Phosphorus Stress on the Growth and Acid Phosphatase Activity of Macrotyloma uniflorum (Lam.) Verdc. Yazhou[J]. Acta Agrestia Sinica, 2021, 29(7): 1462-1468.

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低磷胁迫对崖州硬皮豆生长及酸性磷酸酶活性的影响

Effects of Low Phosphorus Stress on the Growth and Acid Phosphatase Activity of Macrotyloma uniflorum (Lam.) Verdc. Yazhou

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