青贮发酵产物—角鲨烯抑制β-胡萝卜素损失的机理探究

doi:10.11733/j.issn.1007-0435.2023.07.008

草地学报 ›› 2023, Vol. 31 ›› Issue (7): 1988-1995.DOI: 10.11733/j.issn.1007-0435.2023.07.008

青贮发酵产物—角鲨烯抑制β-胡萝卜素损失的机理探究

吴艾丽¹, 肖宇¹, 邵涛¹, 宗成¹, 黄仲永¹, 吴金鑫¹, 刘秦华¹, 荣辉²

1. 南京农业大学草业学院, 江苏南京 210095;
2. 郑州正大饲料有限公司, 河南郑州 450003

收稿日期:2023-02-28 修回日期:2023-05-24 出版日期:2023-07-15 发布日期:2023-08-01
通讯作者: 刘秦华,E-mail:liuqinhua@njau.edu.cn
作者简介:吴艾丽(1996-),女,汉族,安徽六安人,硕士研究生,主要从事饲草调制加工与贮藏研究,E-mail:wal1115@163.com
基金资助:
国家自然科学基金面上项目(31971765)，中央高校基本科研业务费(XUEKEN2022020)资助

Investigation on β-Carotene Loss Inhibition Mechanism by Silage Fermentation Products-Squalene

WU Ai-li¹, XIAO Yu¹, SHAO Tao¹, ZONG Cheng¹, HUANG Zhong-yong¹, WU Jin-xin¹, LIU Qin-hua¹, RONG Hui²

1. College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
2. Zhengzhou Zhengda Feed Co., LTD., Zhengzhou, Henan Province 450003, China

Received:2023-02-28 Revised:2023-05-24 Online:2023-07-15 Published:2023-08-01

摘要/Abstract

摘要： 为筛选和鉴定出抑制β-胡萝卜素损失的青贮发酵产物，本研究探讨了温度(低温3℃~20℃和30℃)和贮藏时间(10，20，40和70 d)对紫花苜蓿(Medicago sativa L.)青贮发酵品质和β-胡萝卜素损失的影响，鉴定了β-胡萝卜素损失低的紫花苜蓿青贮饲料中的主要特异性青贮发酵产物，揭示了其降低β-胡萝卜素损失的机制。结果表明：与低温贮藏相比，30℃贮藏70 d的紫花苜蓿青贮饲料丁酸含量高，发酵品质差，但β-胡萝卜素损失少，含有5种特异性青贮发酵产物。其中角鲨烯含量最高，可抑制乳酸和挥发性脂肪酸对β-胡萝卜素的破坏，且抑制乙酸和丁酸破坏β-胡萝卜素的效果优于乳酸和丙酸。因此，本研究发现了抑制β-胡萝卜素损失的角鲨烯，揭示了其在青贮过程中降低β-胡萝卜素损失的机制。

关键词: 青贮发酵产物, 角鲨烯, β-胡萝卜素

Abstract: To screen and identify silage fermentation products that inhibit β-carotene loss during the fermentation process,the effects of temperature (low temperature,3℃~20℃;30℃) and storage time (10,20,40,and 70 d) on the fermentation quality and β-Carotene loss of alfalfa silage were investigated. The main specific fermentation products in alfalfa silage with low β-carotene loss was identified and the mechanism for reducing β-carotene loss was revealed. The results showed that alfalfa silage stored at 30℃ had high butyric acid content and poor fermentation quality but had less β-carotene loss compared with the low temperature. Furthermore,alfalfa silage stored at 30℃ contained 5 specific silage fermentation products,and squalene content was the highest among them,which can inhibit the β-carotene destruction by lactic acid and volatile fatty acids. The inhibition effect of squalene on β-carotene destruction by acetic acid and butyric acid was better than that of lactic acid and propionic acid. Thus,it was squalene that was found out to have a function to inhibit β-carotene loss in the alfalfa silage,which revealed the mechanism of reducing β-carotene loss during ensiling of alfalfa.

Key words: Silage fermentation products, Squalene, β-Carotene

中图分类号:

S512.6

吴艾丽, 肖宇, 邵涛, 宗成, 黄仲永, 吴金鑫, 刘秦华, 荣辉. 青贮发酵产物—角鲨烯抑制β-胡萝卜素损失的机理探究[J]. 草地学报, 2023, 31(7): 1988-1995.

WU Ai-li, XIAO Yu, SHAO Tao, ZONG Cheng, HUANG Zhong-yong, WU Jin-xin, LIU Qin-hua, RONG Hui. Investigation on β-Carotene Loss Inhibition Mechanism by Silage Fermentation Products-Squalene[J]. Acta Agrestia Sinica, 2023, 31(7): 1988-1995.

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青贮发酵产物—角鲨烯抑制β-胡萝卜素损失的机理探究

Investigation on β-Carotene Loss Inhibition Mechanism by Silage Fermentation Products-Squalene

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