林火强度对兴安落叶松林土壤氮循环功能基因的影响

doi:10.11733/j.issn.1007-0435.2024.03.008

草地学报 ›› 2024, Vol. 32 ›› Issue (3): 726-735.DOI: 10.11733/j.issn.1007-0435.2024.03.008

林火强度对兴安落叶松林土壤氮循环功能基因的影响

舒洋^1,2, 陈金平⁵, 丁兆华⁵, 李航^3,4, 赵鹏武^1,2, 周梅^1,2, 贾文杰^1,2, 张轶超^1,2, 张国臣⁶, 魏江生^2,3,4

1. 内蒙古农业大学林学院, 内蒙古呼和浩特 010019;
2. 内蒙古赛罕乌拉森林生态系统国家定位观测研究站, 内蒙古赤峰 025150;
3. 内蒙古农业大学草原与资源环境学院, 内蒙古呼和浩特 010011;
4. 内蒙古自治区土壤质量与养分资源重点实验室, 内蒙古呼和浩特 010011;
5. 内蒙古根河森林工业有限公司, 内蒙古根河 022350;
6. 内蒙古赤峰市克什克腾旗桦木沟林场, 内蒙古赤峰 025350

收稿日期:2023-07-16 修回日期:2023-10-19 出版日期:2024-03-15 发布日期:2024-04-03
通讯作者: 魏江生,E-mail:weijiangsheng1969@163.com
作者简介:舒洋(1988-)，男，汉族，内蒙古通辽人，讲师，主要从事森林生态学研究，E-mail:shuyang2018@imau.edu.cn
基金资助:
林火干扰对大兴安岭落叶松林土壤微生物氮循环影响机制研究(32001325)；2022年度自治区本级事业单位引进高层次人才科研支持经费(DC2300001275)；高层次/优秀博士人才引进科研启动项目(NDYB2022-18)；森林生态系统多样性的多维度格局及时空分异规律(2022YFF0801801)资助

Effects of Forest Fire Intensity on Functional Genes of Soil Nitrogen Cycling in Larix Gmelinii

SHU Yang^1,2, CHEN Jin-ping⁵, DING Zhao-hua⁵, LI Hang^3,4, ZHAO Peng-wu^1,2, ZHOU Mei^1,2, JIA Wen-jie^1,2, ZHANG Yi-chao^1,2, ZHANG Guo-chen⁶, WEI Jiang-sheng^2,3,4

1. Forestry College of Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China;
2. Saihanwula Forest Ecosystem National Station, Chifeng, Inner Mongolia 025150, China;
3. College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010011, China;
4. Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resource, Hohhot, Inner Mongolia 010011, China;
5. Inner Mongolia Genhe Forest Industry Co. LTD, Genhe, Inner Mongolia 022350, China;
6. Huamugou Forestry, Keshiketeng Banner, Chifeng City, Inner Mongolia, China, Chifeng, Inner Mongolia 025350, China

Received:2023-07-16 Revised:2023-10-19 Online:2024-03-15 Published:2024-04-03

摘要/Abstract

摘要： 为了探究林火强度对土壤氮素循环的影响,本研究以内蒙古大兴安岭兴安落叶松林火烧迹地为研究对象,通过DNA提取及定量PCR分析测定了不同火烧强度样地内土壤氮循环功能基因丰度以及土壤基本的理化性质。结果表明:固氮nifH功能基因丰度随着火烧强度的增加而减少,而硝化amoA-AOA和amoA-AOB功能基因丰度呈相反变化趋势;反硝化nirS,nirK和nosZ功能基因丰度在重度火烧后显著降低,在轻度火烧后显著升高。火烧同样也会对土壤基本理化性质产生显著影响,RDA分析和Pearson相关性分析表明土壤氮循环功能基因与土壤理化因子之间存在密切的联系,有机碳、速效磷、全氮含量和pH值是影响火后土壤氮循环功能基因差异变化的主要因子。本研究为火干扰对土壤氮素循环的影响机制和火后植被快速更新提供数据支撑。

关键词: 火烧强度, 氮循环功能基因, 兴安落叶松, 土壤养分循环

Abstract: To investigate the effect of forest fire intensity on soil nitrogen cycling,the abundance of soil nitrogen cycling functional genes and the physicochemical properties of soil were determined by quantitative PCR in different intensities of fire sample plots in the fire burn site of Larix Gmelinii forest in Da Xing'anling,Inner Mongolia. The results showed that the abundance of nitrogen-fixing nifH functional gene decreased with the increase in fire intensity,while the abundance of nitrifying amoA-AOA and amoA-AOB functional genes showed the opposite trend. Furthermore,the abundance of denitrifying nirS,nirK,and nosZ functional genes was significantly decreased after heavy fire,whereas these functional gene abundances were significantly increased after light fire. Fire also significantly affected the basic physical and chemical properties of soil. It had a close relationship between soil nitrogen cycle functional genes and soil physicochemical factors,in which organic carbon,quick-acting phosphorus,total nitrogen concent,and pH value were the main factors affecting the differential changes of soil nitrogen cycle functional genes after fire based by RDA analysis and Pearson correlation analysis. This study will provide data support for the mechanism of fire disturbance on soil nitrogen cycling and rapid post-fire vegetation regeneration.

Key words: Fire interference, Nitrogen cycling gene, Larix gmelinii, Soil nutrient cycling

中图分类号:

S762

舒洋, 陈金平, 丁兆华, 李航, 赵鹏武, 周梅, 贾文杰, 张轶超, 张国臣, 魏江生. 林火强度对兴安落叶松林土壤氮循环功能基因的影响[J]. 草地学报, 2024, 32(3): 726-735.

SHU Yang, CHEN Jin-ping, DING Zhao-hua, LI Hang, ZHAO Peng-wu, ZHOU Mei, JIA Wen-jie, ZHANG Yi-chao, ZHANG Guo-chen, WEI Jiang-sheng. Effects of Forest Fire Intensity on Functional Genes of Soil Nitrogen Cycling in Larix Gmelinii[J]. Acta Agrestia Sinica, 2024, 32(3): 726-735.

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林火强度对兴安落叶松林土壤氮循环功能基因的影响

Effects of Forest Fire Intensity on Functional Genes of Soil Nitrogen Cycling in Larix Gmelinii

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