响应面法优化火绒草中总黄酮与总酚酸的提取工艺研究

doi:10.11733/j.issn.1007-0435.2024.03.030

草地学报 ›› 2024, Vol. 32 ›› Issue (3): 935-944.DOI: 10.11733/j.issn.1007-0435.2024.03.030

响应面法优化火绒草中总黄酮与总酚酸的提取工艺研究

意如乐¹, 格根图¹, 王志军¹, 胡日查², 百岁², 塞西雅拉图³

1. 内蒙古农业大学草原与资源环境学院, 农业农村部饲草栽培、加工与高效利用重点实验室, 内蒙古呼和浩特 010019;
2. 克什克腾旗林业和草原局, 内蒙古赤峰 025300;
3. 克什克腾旗绿色驿站环境保护监测站, 内蒙古赤峰 025300

收稿日期:2023-09-19 修回日期:2023-11-26 出版日期:2024-03-15 发布日期:2024-04-03
通讯作者: 格根图,E-mail:gegentu@163.com
作者简介:意如乐(1999-)，女，蒙古族，内蒙古赤峰人，硕士研究生，主要从事牧草加工与利用方向研究，E-mail：yirule1207@126.com
基金资助:
财政部和农业农村部：国家牧草产业技术体系(CARS-34)资助

Study on the Extraction Process of Total Flavonoids and Total Phenolic Acids in Leontopodium leontopodioides(Wild.)Beauv

YI Ru-le¹, GE Gen-tu¹, WANG Zhi-jun¹, HU Ri-cha², BAI Sui², SAI Xi Ya La-tu³

1. Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture, College of Grassland Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China;
2. Forestry and Grassland Bureau of Keshiketeng Banner, Chifeng, Inner Mongolia 025300, China;
3. Green Station Environmental Protection Monitoring Station of Keshiketeng Banner, Chifeng, Inner Mongolia 025300, China

Received:2023-09-19 Revised:2023-11-26 Online:2024-03-15 Published:2024-04-03

摘要/Abstract

摘要： 为探究野生火绒草(Leontopodium leontopodioides(Wild.)Beauv)总黄酮与总酚酸最佳提取工艺及抗氧化活性,本试验采用快速溶剂萃取法探索萃取温度、循环次数、加热时间及萃取压力对火绒草总黄酮、总酚酸提取含量的影响,通过单因素试验和响应面设计法确定了活性物质的最佳提取工艺。结果表明:当使用70%乙醇提取火绒草中总黄酮时最佳提取工艺为:萃取温度93℃,循环次数3次,加热时间20 min,萃取压力102 bar,提取含量为(45.45±0.36) mg·g^-1;总酚酸最佳提取工艺为:萃取温度90℃,循环次数3次,加热时间19 min,萃取压力97 bar,提取含量为(23.52±0.34) mg·g^-1。火绒草总黄酮提取液对DPPH自由基和ABTS自由基的IC₅₀为0.012 mg·mL^-1和0.356 mg·mL^-1,具有一定的还原力。本研究可为火绒草活性物质的提取工艺及加工利用提供可靠的理论依据和详实的科学参考。

关键词: 火绒草, 总黄酮, 总酚酸, 快速溶剂萃取, 响应面法优化, 抗氧化活性

Abstract: In order to explore the optimal extraction process and antioxidant activity of total flavonoids and total phenolic acids in wild Leontopodium leontopodioides,the effects of extraction temperature,cycle times,heating time and extraction pressure on the extraction content of total flavonoids and total phenolic acids in Leontopodium leontopodioides were explored by rapid solvent extraction,and the optimal extraction process of active substances was determined by the single-factor experiment and the response surface design. The results showed that the optimal extraction process for the extraction of total flavonoids in Leontopodium leontopodioides using 70% ethanol was 93℃extraction temperature,3 times,20 min extraction time,and 102 bar extraction pressure. Extraction content was (45.45±0.36) mg·g^-1.The best extraction process of total phenolic acid was 90℃extraction temperature,3 times,19 min extraction,and 97 bar extraction pressure. Extraction content was (23.52±0.34) mg·g^-1.The IC₅₀ of Leontopodium leontopodioides total flavonoid extract against DPPH radicals and ABTS radicals were 0.012 mg·mL^-1 and 0.356 mg·mL^-1,respectively. It had a certain reducing power. This study provided a reliable theoretical basis and detailed scientific reference for the extraction process and processing and utilization of the active substance of Leontopodium leontopodioides.

Key words: Leontopodium leontopodioides(Wild.)Beauv, Total flavonoids, Total phenolic acids, Accelerated solvent extraction, Response surface method optimization, Antioxidant activity

中图分类号:

P746.2+2

意如乐, 格根图, 王志军, 胡日查, 百岁, 塞西雅拉图. 响应面法优化火绒草中总黄酮与总酚酸的提取工艺研究[J]. 草地学报, 2024, 32(3): 935-944.

YI Ru-le, GE Gen-tu, WANG Zhi-jun, HU Ri-cha, BAI Sui, SAI Xi Ya La-tu. Study on the Extraction Process of Total Flavonoids and Total Phenolic Acids in Leontopodium leontopodioides(Wild.)Beauv[J]. Acta Agrestia Sinica, 2024, 32(3): 935-944.

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响应面法优化火绒草中总黄酮与总酚酸的提取工艺研究

Study on the Extraction Process of Total Flavonoids and Total Phenolic Acids in Leontopodium leontopodioides(Wild.)Beauv

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[1]	李红, 李波, 李晨阳, 杨曌. 大孔树脂纯化苜蓿总黄酮及纯化前后抗氧化能力比较[J]. 草地学报, 2022, 30(3): 750-757.
[2]	许英一, 王宇, 杨伟光. 紫花苜蓿叶总黄酮提取及抗氧化性[J]. 草地学报, 2018, 26(3): 757-763.
[3]	魏伯平, 张咏梅, 曹致中. 9种豆科牧草总黄酮和总皂苷积累规律的研究[J]. , 2012, 20(1): 88-95.