接种根瘤菌对‘蒙农三叶草1号’根部转录水平的影响

doi:10.11733/j.issn.1007-0435.2024.09.009

草地学报 ›› 2024, Vol. 32 ›› Issue (9): 2759-2768.DOI: 10.11733/j.issn.1007-0435.2024.09.009

• 研究论文 • 上一篇

接种根瘤菌对‘蒙农三叶草1号’根部转录水平的影响

曹克璠¹, 索荣臻¹, 张慧敏¹, 马一鸣¹, 吴倩¹, 包立高², 王明玖¹

1. 内蒙古农业大学草原与资源环境学院/草地资源教育部重点实验室, 内蒙古呼和浩特 010018;
2. 内蒙古自治区农牧业技术推广中心, 内蒙古呼和浩特 010030

收稿日期:2023-12-21 修回日期:2024-03-12 发布日期:2024-10-09
通讯作者: 王明玖,E-mail:wangmj_0540@163.com
作者简介:曹克璠(1998-),男,满族,内蒙古呼伦贝尔人,博士研究生,主要从事牧草种质资源研究,E-mail:caokefan1003@163.com
基金资助:
国家自然科学基金(31960353)资助

The Impact of Rhizobium Inoculation on the Root Transcriptome of ‘Mengnong Clover No.1’

CAO Ke-fan¹, SUO Rong-zhen¹, ZHANG Hui-min¹, MA Yi-ming¹, WU Qian¹, BAO Li-gao², WANG Ming-jiu¹

1. College of Grassland and Resources and Environment/Key Laboratory of Grassland Resources of Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China;
2. Inner Mongolia Agricultural and Animal Husbandry Technology Extension Center, Hohhot, Inner Mongolia 010030, China

Received:2023-12-21 Revised:2024-03-12 Published:2024-10-09

摘要/Abstract

摘要： 为探究‘蒙农三叶草1号’(Trifolium ambiguum Bieb. ‘Mengnong No.1’)与根瘤菌共生过程中相关基因的分子机制及代谢通路，本研究选取了接种根瘤菌的‘蒙农三叶草1号’以及未接种的对照组为研究对象，通过转录组测序，比较了共生与非共生状态下的转录组差异。结果表明，与未接种组相比，接种组共筛选出1105个差异表达基因(Differentially expressed genes，DEGs)。进一步分析发现，这些DEGs涉及多个代谢通路和生物学过程，包括苯丙烷生物合成、氨基糖和核苷酸糖代谢、半胱氨酸和甲硫氨酸代谢等。其中，677个基因上调，428个基因下调。通过实时荧光定量PCR(qRT-PCR)对部分基因进行验证，证实了转录组测序的可靠性。本研究揭示了‘蒙农三叶草1号’在根瘤菌共生状态下的转录组特征，为深入理解植物与根瘤菌共生机制提供了重要参考。

关键词: ‘蒙农三叶草1号’, 接种根瘤菌, 差异表达基因, 转录组学

Abstract: To explore the molecular mechanisms and metabolic pathways of genes related to the symbiotic relationship between ‘Mengnong Clover No.1’ (T. ambiguum Bieb. ‘Mengnong No.1’) and rhizobia,this study selected ‘Mengnong Clover No.1’ inoculated with rhizobia and an uninoculated control group as research subjects. Through transcriptomic sequencing,we compared the transcriptome differences between symbiotic and non-symbiotic states. The results showed that,compared with the uninoculated group,a total of 1105 differentially expressed genes (DEGs) were identified with significant changes of expression levels in all symbiotic samples. Among them,677 genes were upregulated and 428 genes were downregulated. The reliability of the transcriptomic sequencing was confirmed by real-time quantitative PCR (qRT-PCR) for some of these genes. Further analysis revealed that these DEGs were involved in multiple metabolic pathways and biological processes,including biosynthesis of phenylpropanoids,metabolism of amino sugars and nucleotide sugars,as well as cysteine and methionine metabolism. This study reveals the transcriptomic characteristics of ‘Mengnong Clover No.1’ under symbiotic conditions with rhizobia,and provides important references for a deeper understanding of the symbiotic mechanisms between plants and rhizobia.

Key words: ‘Mengnong Clover No. 1’, Rhizobium inoculation, Differentially expressed genes (DEGs), Transcriptomics

中图分类号:

S541.2

曹克璠, 索荣臻, 张慧敏, 马一鸣, 吴倩, 包立高, 王明玖. 接种根瘤菌对‘蒙农三叶草1号’根部转录水平的影响[J]. 草地学报, 2024, 32(9): 2759-2768.

CAO Ke-fan, SUO Rong-zhen, ZHANG Hui-min, MA Yi-ming, WU Qian, BAO Li-gao, WANG Ming-jiu. The Impact of Rhizobium Inoculation on the Root Transcriptome of ‘Mengnong Clover No.1’[J]. Acta Agrestia Sinica, 2024, 32(9): 2759-2768.

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https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2024/V32/I9/2759

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接种根瘤菌对‘蒙农三叶草1号’根部转录水平的影响

The Impact of Rhizobium Inoculation on the Root Transcriptome of ‘Mengnong Clover No.1’

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