草地学报

草地学报 ›› 2026, Vol. 34 ›› Issue (10): 3604-3615.DOI: 10.11733/j.issn.1007-0435.2026.10.008

• 研究论文 • 上一篇    

燕麦幼苗响应干旱胁迫的生理与转录组学分析

何雨飞1, 呼延美静1, 唐川江2, 张清1, 刘博1, 吕奥1, 刘伟1   

  1. 1. 四川农业大学草业科技学院, 四川 成都 611130;
    2. 四川省草业技术研究推广中心, 四川 成都 610041
  • 收稿日期:2025-12-12 修回日期:2026-01-24 发布日期:2026-10-10
  • 通讯作者: 刘伟, E-mail: lwgrass@126.com
  • 作者简介:何雨飞（2002-），男，汉族，湖北潜江人，硕士研究生，主要从事饲草创新与推广研究，E-mail：2013863478@qq.com
  • 基金资助:
    现代农业产业技术体系四川饲草创新团队建设专项资金（SCCXTD-2024-16）资助

Physiological and Transcriptomic Analysis of Oat Seedlings in Response to Drought Stress

HE Yu-fei1, HU YAN Mei-jing1, TANG Chuan-jiang2, ZHANG Qing1, LIU Bo1, LÜ Ao1, LIU Wei1   

  1. 1. College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China;
    2. Sichuan Grass Industry Technology Research and Promotion Center, Chengdu, Sichuan Province 610041, China
  • Received:2025-12-12 Revised:2026-01-24 Published:2026-10-10

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摘要： 本研究以燕麦（Avena sativa）为材料，通过模拟干旱胁迫，系统分析了其生理响应与转录组变化。生理结果表明，干旱胁迫导致叶片相对含水量（Relative water content，RWC）显著降低，相对电导率（Relative electrical conductivity，REC）上升，并诱导了超氧化物歧化酶（Superoxide dismutase，SOD）、过氧化物酶（Peroxidase，POD）和过氧化氢酶（Catalase，CAT）等抗氧化酶活性的动态变化。转录组测序（RNA-Seq）揭示了大量基因的表达变化，其中369个为各胁迫时间点共有差异表达基因（Differentially expressed genes，DEGs），主要富集于植物激素信号转导、丝裂原活化蛋白激酶（Mitogen-activated protein kinase，MAPK）信号通路和淀粉蔗糖代谢途径。进一步通过加权基因共表达网络分析（Weighted gene co-expression network analysis，WGCNA），筛选出1个与表型性状正相关的核心模块，并从中识别出9个枢纽基因。这些基因主要参与细胞壁强化、渗透保护和代谢调节等过程，在燕麦应对干旱胁迫中发挥核心作用，为深入理解燕麦抗旱分子机制提供了理论基础。

关键词: 燕麦, 干旱胁迫, 转录组, WGCNA

Abstract: This study utilized oat （Avena sativa） as the material to systematically analyze its physiological and transcriptomic responses under simulated drought stress. The physiological results indicated that drought stress caused a significant decrease in leaf relative water content （RWC） and an increase in relative electrical conductivity （REC）， and induced dynamic changes in the activities of antioxidant enzymes such as superoxide dismutase （SOD）， peroxidase （POD）， and catalase （CAT）. Transcriptome sequencing （RNA-Seq） revealed that the expression of numerous genes were changed dynamically， of which 369 were common differentially expressed genes （DEGs） across all stress time points. These common DEGs were primarily enriched in plant hormone signal transduction， the mitogen-activated protein kinase （MAPK） signaling pathway， and starch and sucrose metabolism. Furthermore， weighted gene co-expression network analysis （WGCNA） identified one core module that was positively correlated with phenotypic traits， from which nine hub genes were identified. These genes are mainly involved in processes such as cell wall reinforcement， osmotic protection and metabolic regulation， playing a pivotal role in the response of oat to drought stress. This research provides a theoretical basis for an in-depth understanding of the molecular mechanisms underlying drought resistance in oats.

Key words: Oat, Drought stress, Transcriptome, WGCNA

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