红蓼提取物杀虫活性及其对粘虫的作用方式

doi:10.11733/j.issn.1007-0435.2009.03.021

›› 2009, Vol. 17 ›› Issue (3): 377-382.DOI: 10.11733/j.issn.1007-0435.2009.03.021

红蓼提取物杀虫活性及其对粘虫的作用方式

张新瑞¹, 胡冠芳², 刘敏艳², 李玉奇², 余海涛²

1. 甘肃农业大学草业学院昆虫学系, 兰州, 730070;
2. 甘肃省农业科学院植物保护研究所, 兰州, 730070

收稿日期:2008-04-23 修回日期:2009-03-10 出版日期:2009-06-15 发布日期:2009-06-15
通讯作者: 胡冠芳,E-mail:huguanfang@126.com
作者简介:张新瑞(1964- ),男,甘肃省武山县人,博士研究生,研究方向为植物保护,E-mail:zhxinrui@sohu.com
基金资助:
甘肃省科学事业费科研项目(QS041-C31-06);甘肃省农业生物技术研究与应用开发项目(2006)

Insecticidal Effects of Extracts from Polygonum orientale L. and Their Action Ways against Mythimna separate Walker

ZHANG Xin-rui¹, HU Guan-fang², LIU Min-yan², LI Yu-qi², YU Hai-tao²

1. Department of Entomology, Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu Province 730070, China

Received:2008-04-23 Revised:2009-03-10 Online:2009-06-15 Published:2009-06-15

摘要/Abstract

摘要： 采用虫体浸渍法和夹毒叶碟法等方法研究红蓼(Polygonum orientale L.)提取物杀虫活性及其对粘虫的作用方式,为利用该植物研制新型植物源杀虫剂提供理论依据。结果表明,红蓼的最高杀虫活性部位是种子和秋季茎秆,甲醇提取物稀释5倍液对粘虫(Mythi mnaseparate Walker)5龄幼虫的触杀死亡率分别为21.18%和20.29%。种子不同极性溶剂提取物的触杀作用以乙醇最强,校正死亡率可达41.97%;秋季茎秆则以甲醇最强,校正死亡率为20.29%。种子和秋季茎秆乙酸乙酯萃取物的触杀死亡率分别为88.89%和59.20%,二者的LC50值分别为8.6466 g(浸膏)/L和29.0484 g(浸膏)/L。种子和秋季茎秆乙酸乙酯萃取物稀释10倍液对菜粉蝶(Pieris rapae L.)4龄幼虫的触杀死亡率均达100%,种子乙酸乙酯萃取物稀释5倍液对小菜蛾(Plutella xylostella L.)3龄幼虫、棉铃虫(Helicoverpa armigera Hbner)和甘蓝夜蛾(Mamestra brassicae L.)5龄幼虫的触杀死亡率分别为70.27%、87.50%和90.00%。种子乙酸乙酯萃取物对粘虫3龄幼虫的胃毒死亡率可达57.14%,秋季茎秆乙酸乙酯萃取物对3龄幼虫的生长发育抑制率为30.45%。

关键词: 红蓼, 提取物, 杀虫活性, 粘虫, 作用方式

Abstract: Insecticidal activities of Polygonum orientale L.were seldom reported at home and abroad and the action ways of extracts from P.orientale against Mythimna separate Walker has not been investigated up till now.Study on insecticidal activities and action ways of the extracts can provide theoretical basis for formulating new type of botanical insecticides.The paper conducted insecticidal activities of extracts from P.orientale and their action ways against M.separate using immersion and sandwich methods.The results show that the extracts from seeds and stems in autumn had the highest effects and the contact mortality of methanol extract(diluted 5 times) against the 5th instar larvae of M.separate were 21.18% and 20.29%,respectively.Among the different solvent extracts,the ethanol extract from seeds had the strongest contact effect against the larvae,the adjusted mortality was 41.97%;the adjusted mortality of the methanol extract from stems in autumn was 20.29%.The adjusted mortalities of ethyl acetate extract from seeds and stems in autumn were 88.89% and 59.20%,respectively;the values of LC50 were 8.6466 and 29.0484 g·L^-1(extracts).The contact mortalities of the two extracts(diluted 10 times) were all 100% against the 4th instar larvae of Pieris rapae L.The contact mortalities of ethyl acetate extract(diluted 5 times) from seeds were 70.27,87.5,and 90.00% against the 3rd instar larvae of Plutella xylostella L.,the fifth instar larvae of Helicoverpa armigera Hübner and Mamestra brassicae L.The ethyl acetate extract from seeds had stomach poisoning effect against the 3rd instar larvae and the adjusted mortality was 57.14%;the inhibition rate of ethyl acetate extract from stems in autumn against the 3rd instar larvae was 30.45%.

Key words: Polygonum orientale L., Extract, Insecticidal activity, Mythimna separate Walker, Action way

中图分类号:

Q946.91

张新瑞, 胡冠芳, 刘敏艳, 李玉奇, 余海涛. 红蓼提取物杀虫活性及其对粘虫的作用方式[J]. , 2009, 17(3): 377-382.

ZHANG Xin-rui, HU Guan-fang, LIU Min-yan, LI Yu-qi, YU Hai-tao. Insecticidal Effects of Extracts from Polygonum orientale L. and Their Action Ways against Mythimna separate Walker[J]. , 2009, 17(3): 377-382.

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参考文献

[1] 张宏,袁静.皱叶酸模根提取物的杀菌活性研究[D].西安:中国化工学会农药专业委员会第12届年会论文集,2005:449
[2] 杨仓良主编.毒药本草[M].北京:中国中医药出版社,1993
[3] 张继振.红蓼果实黄酮化合物的研究[J].中草药,1990,21(8):7
[4] 李友莲,李秀岚,魏清霞.红蓼提取物对桃蚜的杀虫活性研究[J].山西农业大学学报(自然科学版),2004,24(3):234-236
[5] 李秀岚,李友莲.红蓼提取物对桃蚜拒食活性及触角电位的研究[J].山西农业大学学报2005,25(3):228-230
[6] 李友莲,魏清霞,肖鹏飞.红蓼主要部位提取物对桃蚜的拒食活性研究[D].北京:中国植物保护学会2007年学术年会论文集,2007:429-436
[7] 林妍妍.东方蓼提取物杀虫杀菌活性初探[J].农药,2007,46(3):211-213
[8] 刘东泽,赵杰,刘志诚,等.20种植物提取物对几种昆虫的拒食作用[J].农药学学报,2003,5(3):89-93
[9] 中华人民共和国农业部2006-07-10发布.中华人民共和国农业行业标准(NY/T 1154.6-2006):农药室内生物测定试验准则杀虫剂第6部分:杀虫活性试验浸虫法[M].北京:中国农业出版社,2006
[10] 袁林,薛明,刑建,等.黄荆提取物对几种害虫的杀虫活性[J].农药,2004,43(2):70-72
[11] 张国洲,王亚维,徐汉虹,等.瑞香狼毒提取物对昆虫的生物活性研究[J].湖南农业大学学报,2004,26(3):190-192
[12] 牛树君,胡冠芳,刘敏艳,等.毛曼陀罗对粘虫和蚜虫的杀虫活性研究[J].甘肃农业科技,2008,(9):3-6
[13] 罗都强,张兴.雷公藤非生物碱分离及对粘虫作用方式研究[J].西北农林科技大学学报(自然科学版),2001,29(4):81-84
[14] 张兴,潘文亮.缓效型杀虫剂室内生物测定的计算和评价[J].北京农业科学,1989,(3):6-10
[15] 李玉峰,朱丽梅,倪珏萍,等.JS118的杀虫活性与作用方式研究[J].现代农药,2003,2(4):13,26
[16] 魏红梅,张兴.砂地柏精油的熏蒸杀虫作用研究[A].第二届全国植物农药暨第六届药剂毒理学术讨论会论文集[C].陕西杨陵:2001,231-234
[17] Grainge M,Ahmed S.Handbook of plants with pest control properties[M].New York:John Wiley,1988
[18] Jacobson M.Botanical pesticides past,present and future[A].In:Arnason J T.ed.Insecicide of plant origin.[M].Amer.Chem.Soci.,Washington DC,1989
[19] Pillmoor B O,Wright K,Terry S A.Natural products as a source of agrochemicals and leads for chemical synthesis[J].Pestic.Sci.,1993,39:131-140
[20] 张敏恒.国外农药开发的一些特点[J].农药,1993,38(10):27-30
[21] 张兴.试论无公害农药[J].西北农业大学学报,1995,23(6):90-95
[22] 屠豫钦.农药和化学防治的"三E"问题-效力、效率和环境[J].农药译丛,1998,20(3):1-5
[23] 郑永权,姚建仁,邵向东.21世纪农药展望[J].植物保护,1998,4:39-42

红蓼提取物杀虫活性及其对粘虫的作用方式

Insecticidal Effects of Extracts from Polygonum orientale L. and Their Action Ways against Mythimna separate Walker

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