草地学报

›› 2003, Vol. 11 ›› Issue (2): 146-153.DOI: 10.11733/j.issn.1007-0435.2003.02.010

• 研究论文 • 上一篇    下一篇

退化梯度对克氏针茅种群遗传分化的影响

韩冰1, 王俊2, 赵萌莉3, 许志信3, 索培芬1   

  1. 1. 内蒙古农业大学生物工程学院, 呼和浩特, 010019;
    2. 内蒙古农业大学动物科技学院, 呼和浩特, 010019;
    3. 内蒙古农业大学生态环境学院, 呼和浩特, 010019
  • 收稿日期:2002-08-29 修回日期:2002-11-19 出版日期:2003-05-15 发布日期:2003-05-15
  • 作者简介:韩冰(1970- ),女,在读博士生,主要从事植物分子生物学科研工作
  • 基金资助:
    国家自然基金(30060015);内蒙古自然基金(20001303);“农业部草地资源生态重点实验室”资助

Genetic Differentiation of Stipa Krylovii in Different Degraded Soil

HAN bing1, WANG Jun2, ZHAO Meng-li3, XU Zhi-xin3, SUO Pei-feng1   

  1. 1. College of Biology, Inner Mongolia Agricultural University Huhhot, Inner Mongolia Autonomous Region 010019, China;
    2. College of Animal Science, Inner Mongolia Agricultural University Huhhot, Inner Mongolia Autonomous Region 010019, China;
    3. College of Ecology and Environment Science, Inner Mongolia Agricultural University Huhhot, Inner Mongolia Autonomous Region 010019, China
  • Received:2002-08-29 Revised:2002-11-19 Online:2003-05-15 Published:2003-05-15

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摘要： 对重度退化、轻度退化和正常样地的克氏针茅种群进行RAPD研究。结果表明,3个种群扩增条带总数、多态位点比率、种群内稀有位点数、遗传多样性和基因多样性均是正常种群>轻度退化种群>重度退化种群。用Shannon指数估算3个种群的遗传多样性,其中72.39%的遗传变异存在于种群内,26.89%存在于种群间。重度退化与轻度退化种群,重度退化与正常种群间的遗传相似系数分别为0.8993和0.8159。在重度退化情况下克氏针茅种群遗传结构发生了一定的遗传分化。

关键词: 克氏针茅, 种群, 多态位点, 遗传分化

Abstract: Using DNA (RAPD) analysis, a study of 3 Stipa kryvolii populations from a plot of heavily degraded grassland, a plot of lightly degraded grassland, and one of normal conditions was undertaken. The result shows that the sequence in terms of the total amplified bands, polymorphic loci percentage, rare loci numbers within each population, genetic diversity, and gene diversity among the 3 populations is: those of the normal grassland>lightly degraded one>heavily degraded one. Shannon index indicates that 72.39% genetic diversity exist within each population, while only 26.89% exist among the populations. The phenotypic similarity coefficiency between the heavily degraded grassland population and the lightly degraded one amounts to 0.8993, and that between the heavily degraded and the normal condition ones is 0.8159. Heavy degradation conditions appear to lead to some genetic differentiation in the genetic structure of Stipa krylovii.

Key words: Stipa krylovii, Population, Polymorphic loci, Genetic differentiation

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