不同地理种群的亚洲小车蝗mtDNA COⅠ基因序列及其相互关系

doi:10.11733/j.issn.1007-0435.2011.05.023

›› 2011, Vol. 19 ›› Issue (5): 846-851.DOI: 10.11733/j.issn.1007-0435.2011.05.023

不同地理种群的亚洲小车蝗mtDNA COⅠ基因序列及其相互关系

高书晶¹, 李东伟², 刘爱萍¹, 闫志坚¹, 徐林波¹

1. 中国农业科学院草原研究所, 内蒙古呼和浩特010010;
2. 内蒙古农业大学农学院, 内蒙古呼和浩特010019

收稿日期:2010-06-30 修回日期:2011-04-01 出版日期:2011-10-15 发布日期:2012-07-12
作者简介:高书晶(1978- ),女,内蒙古包头人,副研究员,博士,从事有害生物综合治理研究,E-mail:shujinggao688@163.com
基金资助:
中央级公益性科研院所基本科研业务费专项资金项目(1610332011010)资助

Sequence Analysis of mtDNA COⅠ Gene and Relationships of Geographical Populations of Oedaleus asiaticu in Inner Mongolia

GAO Shu-jing¹, LI Dong-wei², LIU Ai-ping¹, YAN Zhi-jian¹, XU Lin-bo¹

1. Grassland Research Institute of Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia 010010, China;
2. Inner Mongolia Agriculture University, Insititite of Agricultuer, Hohhot, Inner Mongolia 010019, China

Received:2010-06-30 Revised:2011-04-01 Online:2011-10-15 Published:2012-07-12

摘要/Abstract

摘要： 亚洲小车蝗(Oedaleus asiaticus)是北方草原重要的经济害虫,应用PCR直接测序法对7个不同地理种群的亚洲小车蝗共32个个体的mtDNA COⅠ基因中一段473 bp的序列进行测序。通过比较其同源性,计算核苷酸组成,并用槌角蝗科的宽须蚁蝗(Myrmeleotettix palpalis)和斑腿蝗科的鼓翅皱膝蝗(Angaracris barabensis)作外群构建NJ分子系统树。结果表明:在获得的亚洲小车蝗473 bp的序列中,A+T约占71.7%,其中29个核苷酸位点存在变异(约占所测核苷酸的6.3%)。就每个氨基酸密码子来看,第3位点的A+T含量最高。由NJ树显示,亚洲小车蝗mtDNA COⅠ序列不同单倍型之间有一定的分歧,形成不同的簇类关系,其分枝与地理分布没有直接的对应关系,但总体上看,这种簇类关系基本上呈平行分布,没有明显的地域性差别。

关键词: 亚洲小车蝗, 地理种群, mtDNA, COⅠ基因, 遗传关系

Abstract: Oedaleus asiaticu is an important pest in northern grasslands.The 473 bp fragments of mtDNA COⅠ gene from 32 individuals of 7 geographical populations in Inner Mongolia are sequenced.The molecular phylogenetic tree is constructed by the Neighbor-Joining method using Myrmeleotettix palpalis and Angaracris barabensis as outgroup species.Sequence analyses indicate that A+T is about 71.7% and 29 nucleotide sites are substituted in the 473 bp fragment of Oedaleus asiaticu(about 6.3% of sequenced nucleotide).A+T in the third site is much higher than the other two sites.The NJ tree suggests that different individuals from 7 areas of Inner Mongolia are clustered into a different group.However,no clear relationships between heliotypes and geographic regions are inferred by the phylogenetic tree.There are no significant differences between the clusters of different areas since the branches of clusters are parallel.

Key words: Oedaleus asiaticus, Geographical populations, mtDNA, COⅠ, Genetic relationship

中图分类号:

S433.2

高书晶, 李东伟, 刘爱萍, 闫志坚, 徐林波. 不同地理种群的亚洲小车蝗mtDNA COⅠ基因序列及其相互关系[J]. , 2011, 19(5): 846-851.

GAO Shu-jing, LI Dong-wei, LIU Ai-ping, YAN Zhi-jian, XU Lin-bo. Sequence Analysis of mtDNA COⅠ Gene and Relationships of Geographical Populations of Oedaleus asiaticu in Inner Mongolia[J]. , 2011, 19(5): 846-851.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2011.05.023

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2011/V19/I5/846

参考文献

[1] 郑哲民,夏凯龄.中国动物志:昆虫纲:第十卷:直翅目:蝗总科[M].北京:科学出版社,2002:121-123
[2] 吴虎山,能乃扎布.呼伦贝尔市草地蝗虫[M].北京:中国农业出版社,2009:64-66
[3] 潘建梅.内蒙古草原蝗虫发生原因及防治对策[J].中国草地,2002,24(6):66-69
[4] 吴效东.乌兰察布市蝗虫发生规律及危害特点[J].内蒙农业科技,2007(6):69-70
[5] 李广.亚洲小车蝗为害草场损失估计分析的研究[D].北京:中国农业科学院,2007
[6] 卢辉,韩建国,张泽华.典型草原亚洲小车蝗危害对植物补偿生长的作用[J].草业科学,2008,25(5):112-116
[7] 卢辉,韩建国.典型草原三种蝗虫种群死亡率和竞争的研究[J].草地学报,2008,16(5):480-484
[8] 高书晶,刘爱萍,徐林波,等.8种生物农药对草原蝗虫的田间防治效果评价[J].草业科学,2011,28(2):304-307
[9] 郑先云.斑翅蝗科部分种群遗传结构研究[D].太原:山西大学,2006
[10] 邰丽华,贾建宇,王塔娜,等.内蒙古中东部地区亚洲小车蝗3个种群的遗传多样性分析[J].华北农学报,2011,26(1):122-126
[11] 蒋湘,买买提明,张龙.夜间迁飞的亚洲小车蝗[J].草地学报,2003,11(1):75-77
[12] Danforth B N,Mitchell P L,Packer L.Mitochondrial DNA differentiation between two Gryptic halictus (Hymenoptera:Halictidae)species[J].Annals of the Entomological Society of America,1998,91(4):387-391
[13] 张方,米志勇.动物线粒体DNA的分子生物学研究进展[J].生物工程进展,1998,18(3):25-31
[14] Lunt D H,Ibrahim K M,Hewitt G M.mtDNA phylogegraphy and post-glacial patterns of subdivision in the meadow grasshoppcr Chorthippus parallelus[J].Heredity,1998,80:633-641
[15] Villalba S,Lobo J M,Martin-Piera F,etal.Phylogenetic relationships of Iberian dung beetles (Coleoptera:Scarabaeinae):insights on the evolution of nesting behavior[J].Journal of Molecular Evolution,2002,55(1):116-126
[16] 任竹梅,马恩波,郭亚平.不同地域小稻蝗mtDNA部分序列及其相互关系[J].昆虫学报,2003,46(1):51-57
[17] 印红,刘晓丽,手彦芳,等.一种改进的昆虫基因组DNA的提取方法[J].河北大学学报(自然科学版),2002,22(1):80-83
[18] 张民照,康乐.飞蝗总DNA的提取及其RAPD分析条件的摸索[J].动物学研究,2001,22(1):20-26
[19] Simon C,Frati E,Bechenbach A,etal.Evolution,weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers[J].Annals of the Entomological Society of America,1994,87:651-701
[20] Zhang D X,Hewitt G M.Nuclear integrations:challenges for mito-chondrial DNA markers[J].TREE,1996,11 (6):247-251
[21] 任竹梅,马恩波,郭亚平,等.山稻蝗及相关物种Cyt b基因序列及其遗传关系[J].遗传学报,2002,29(6):507-513
[22] Dusfour L,Linton Y M,Cohuet A,et al.Molecular evidence of speciation between island and continental populations of Anopheles (Cellia) sundaicus (Diptera:Culicidae),a principal malaria vector taxon in Southeast Asia[J].Journal of Medical Entomology,2004,41 (3):287-295
[23] 代金霞.线粒体b基因与昆虫分子系统学研究[J].四川动物,2005,27(2):62-67
[24] Crozier R H,Crozier Y C.The cytochrome b and ATPase genes of homeybee mitochondrial DNA[J].Molecular Biology and Evolution,1992,9:474 482
[25] Hugall.Evolution of the AT-rich mitochondrial DNA of the root knot nematodeMeloidogynehapla[J].Molecular Biology and Evolution,1997,14 (1):40-48
[26] Flook P K,Rowll G H F,Gellissen G.The sequence,organization,and evolution of the locusta migratoria mitochondrial genome[J].Journal of Molecular Evolution,1995,41:928-941
[27] Cheke R A.A migrant pest in the Sahel:the Senegalese grasshopper Oedaleus enegalensis[J].Philosophical Transactions of the Royal Society of London Series B:Biological Sciences,1990,328(1251):539-553

不同地理种群的亚洲小车蝗mtDNA COⅠ基因序列及其相互关系

Sequence Analysis of mtDNA COⅠ Gene and Relationships of Geographical Populations of Oedaleus asiaticu in Inner Mongolia

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价

[1]	梁国玲, 周青平, 刘文辉, 耿帆, 颜红波, 陈仕勇. 燕麦I-D品系及其亲本的染色体核型分析[J]. 草地学报, 2016, 24(5): 1026-1031.
[2]	刘贵河, 王国杰, 汪诗平, 张英俊, 邵新庆, 宛新荣, 郝树广. 内蒙古典型草原主要草食动物食性及其营养生态位研究——以大针茅群落为例[J]. , 2013, 21(3): 439-445.
[3]	金娟, 王森山, 贺春贵. 西北不同地理种群红色型豌豆蚜的遗传多样性[J]. , 2013, 21(2): 406-412.
[4]	俞靓, 吴艳芹, 魏琳, 陈奥, 井赵斌, 程积民. 黄土高原半干旱区不同本氏针茅种群抗旱性研究[J]. , 2012, 20(6): 1065-1071.
[5]	刘朝阳, 刘艳, 曹广春, 牙森·沙力, 吴惠惠, 王广君, 张泽华. 模拟亚洲小车蝗为害与草地植被恢复能力研究[J]. , 2012, 20(4): 772-777.
[6]	蒋湘, 买买提明, 张龙. 夜间迁飞的亚洲小车蝗[J]. , 2003, 11(1): 75-77.