草地学报

草地学报 ›› 2014, Vol. 22 ›› Issue (6): 1288-1294.DOI: 10.11733/j.issn.1007-0435.2014.06.022

• 研究论文 • 上一篇    下一篇

3种外源培养基材料对苜蓿根瘤菌活性的影响

周可1, 邓波1, 洪鹤2, 张英俊1, 周冀琼1, 石茂玲1, 邓罗伟1   

  1. 1. 中国农业大学动物科技学院, 北京 100193;
    2. 中国农业杂志社, 北京 100036
  • 收稿日期:2014-01-23 修回日期:2014-05-12 出版日期:2014-12-15 发布日期:2014-12-01
  • 通讯作者: 邓波
  • 作者简介:周可(1988-),女,河南商丘人,硕士,主要从事草地资源与利用的研究,E-mail: nicole2054@outlook.com
  • 基金资助:

    国家牧草产业技术体系(CARS-35);国家863计划(2012AA101802);公益性行业(农业)科研专项(201003061)资助

Effects of Three Kinds of Foreign Media Materials on the Activity of Alfalfa Rhizobium

ZHOU Ke1, DENG Bo1, HONG He2, ZHANG Ying-jun1, ZHOU Ji-qiong1, SHI Mao-ling1, DENG Luo-wei1   

  1. 1. Department of Grassland Science, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
    2. Journal of Agriculture China Agency, Beijing 100036, China
  • Received:2014-01-23 Revised:2014-05-12 Online:2014-12-15 Published:2014-12-01

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摘要：

以紫花苜蓿(Medicago sativa)根瘤菌ACCC17631和ACCC17676为供试菌株,采用单因素试验和正交试验设计,研究了KH2PO4、黄腐酸、硝酸稀土对苜蓿根瘤菌活性的影响,以期得到最优培养基材料组合.结果表明:KH2PO4可极显著促进紫花苜蓿根瘤菌菌株ACCC17631的生长(P<0.01),最适添加浓度为300 mg·L-1,对菌株ACCC17676的生长促进效果不显著;黄腐酸对2株菌的生长具有极显著促进作用(P<0.01),菌株ACCC17631的最适添加浓度为0.03%,菌株ACCC17676的最适添加浓度为0.02%;硝酸稀土可促进菌株ACCC17631生长,最适添加浓度为200 mg·L-1;低浓度硝酸稀土对菌株ACCC17676具极显著生长促进作用(P<0.01),最适添加浓度为400 mg·L-1.菌株ACCC17631最优材料组合为100 mg·L-1 KH2PO4、0.03%黄腐酸与200 mg·L-1硝酸稀土;菌株ACCC17676的最优材料组合为300 mg·L-1 KH2PO4、0.02%黄腐酸与400 mg·L-1硝酸稀土.

关键词: 苜蓿根瘤菌, 黄腐酸, 硝酸稀土, 磷酸二氢钾

Abstract:

The effects of Monopotassium Phosphate, Fulvic Acid and Rare Earth Nitrate on the activity of alfalfa rhizobium were investigated using single factor and orthogonal experiment designs with Alfalfa rhizobium strain ACCC17631 and ACCC17676 as experimental materials in order to screen the optimum combination of medium. The results showed that Monopotassium Phosphate had significant influence on the activity of strain ACCC17631(P<0.01),the optimum concentration was 300 mg·L-1, and no significant influence on the activity of strain ACCC17676. Fulvic Acid had highly significant influence on the activity of both test strains(P<0.01), the optimum concentration was 0.03% for strain ACCC17631 and 0.02% for strain ACCC17676. Rare Earth Nitrate could promote the growth of strain ACCC17631, the optimum concentration was 200 mg·L-1, and had highly significant influence on the activity of strain ACCC17676(P<0.01), the optimum concentration was 400 mg·L-1. The optimum combination of medium for ACCC17631 was 100 mg·L-1of Monopotassium Phosphate, 0.03% of Fulvic Acid and 200 mg·L-1 of Rare Earth Nitrates, for ACCC17676 was 300 mg·L-1 of Monopotassium Phosphate, 0.02% of Fulvic Acid and 400 mg·L-1 of Rare Earth Nitrates.

Key words: Alfalfa rhizobium, Fulvic acid, Rare earth nitrates, Monopotassium phosphate

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