溶磷菌分泌有机酸与溶磷能力相关性研究

doi:10.11733/j.issn.1007-0435.2015.05.020

草地学报 ›› 2015, Vol. 23 ›› Issue (5): 1033-1038.DOI: 10.11733/j.issn.1007-0435.2015.05.020

溶磷菌分泌有机酸与溶磷能力相关性研究

张英¹, 芦光新¹, 谢永丽¹, 姚拓², 荣良燕², 朱颖²

1. 青海大学农牧学院三江源区高寒草地生态省部共建重点实验室, 青海西宁 810016;
2. 甘肃农业大学草业学院, 甘肃兰州 730070

收稿日期:2014-12-24 修回日期:2015-06-14 出版日期:2015-10-15 发布日期:2015-12-01
通讯作者: 姚拓
作者简介:张英(1977-),男,青海贵德人,博士,副教授,主要从事草地生态和植物根际促生菌的研究, E-mail:zhangying2019@126.com
基金资助:
国家自然科学基金项目(31260025)(31360584);青海省自然科学基金项目(2014-ZJ-927Q);青海大学博士科研基金项目资助

The Relationship between Organic Acid Secreted from Phosphorus-solubilizing Bacteria and The Phosphate-solubilizing Ability

ZHANG Ying¹, LU Guang-xin¹, XIE Yong-li¹, YAO Tuo², RONG Liang-yan², ZHU Ying²

1. Agriculture and Animal Husbandry college of Qinhai University, Key Laboratory of Alpine Grassland Ecosystem in the Sanjiangyuan Source Area Jointly Funded by Qinghai Province and Ministry of Education, Xining, Qinhai Province 810016, China;
2. College of Prataculture Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China

Received:2014-12-24 Revised:2015-06-14 Online:2015-10-15 Published:2015-12-01

摘要/Abstract

摘要：

为了研究溶磷菌的溶磷机理,探究溶磷菌分泌有机酸的种类和数量与溶磷能力的相关性。利用PKO无机磷液体培养基对筛选自兰州周边的苜蓿(Medicago sativa)、白三叶(Trifolium repens)和红三叶(Trifolium pratense)根际的6株溶磷菌进行液体培养,采用钼蓝比色法和高效液相色谱法测定溶磷量及分泌有机酸的种类和数量。结果表明,各菌株溶磷量差异显著(P<0.01),有效磷增量在106.53~373.44 mg·L^-1之间。供试菌株分泌有机酸的种类主要有草酸、酒石酸、苹果酸、乳酸、乙酸、柠檬酸和丁二酸等,但分泌有机酸的种类和数量差异较大。溶磷菌的溶磷量与总有机酸量间存在一定相关性,但有效磷增量并不完全由总有机酸量来决定,有机酸的种类和数量对溶磷量也有一定的影响,各种有机酸对溶磷量的影响程度和作用机理有待进一步研究。

关键词: 溶磷菌, 有机酸, 溶磷能力

Abstract:

In order to investigate the phosphorus solubilizing mechanism of phosphate solubilizing bacteria, the relationship between phosphate solubilization ability and the category of organic acid produced by phosphate solubilizing bacteria was investigated. Six phosphate solubilizing bacteria screened from rhizosphere of Medicago sativa, Trifolium repens, and Trifolium pratense around Lanzhou area were cultured using PKO inorganic phosphorus liquid media. The phosphate solubilizing capacity and category of organic acids produced by bacteria were checked by means of molybdenum blue colorimetry and HPLC, respectively. The results showed that the phosphate solubilizing capacity of each bacteria presented significant difference(P<0.01) and the increment of effective phosphate was between 106.53~373.44 mg·L^-1. The category of organic acids produced by phosphorus solubilizing bacterial included oxalic acid, tartaric acid, malic acid, lactic acid, acetic acid, citric acid, succinic acid and so on. However, the category and quantity of organic acids secreted by different strains had significant difference. Relativity was discovered between phosphate solubilizing capacity and total organic acids. The increment of effective phosphate was not entirely affected by total organic acid, and phosphate solubilizing capacity was affected by category and quantity of organic acids. Nonetheless, the influence degree and mechanism of organic acids effected phosphate solubilizing ability still needs further investigation.

Key words: Phosphate solubilizing bacteria, organic acids, Phosphate solubilizing ability

中图分类号:

Q939.96

张英, 芦光新, 谢永丽, 姚拓, 荣良燕, 朱颖. 溶磷菌分泌有机酸与溶磷能力相关性研究[J]. 草地学报, 2015, 23(5): 1033-1038.

ZHANG Ying, LU Guang-xin, XIE Yong-li, YAO Tuo, RONG Liang-yan, ZHU Ying. The Relationship between Organic Acid Secreted from Phosphorus-solubilizing Bacteria and The Phosphate-solubilizing Ability[J]. Acta Agrestia Sinica, 2015, 23(5): 1033-1038.

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溶磷菌分泌有机酸与溶磷能力相关性研究

The Relationship between Organic Acid Secreted from Phosphorus-solubilizing Bacteria and The Phosphate-solubilizing Ability

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