草地学报

草地学报 ›› 2025, Vol. 33 ›› Issue (2): 410-418.DOI: 10.11733/j.issn.1007-0435.2025.02.009

• 研究论文 • 上一篇    

1株矮生嵩草根际高效溶磷菌的筛选、鉴定与促生效果研究

武燕茹1,2, 杨文权3, 刘家庆1, 寇伟良1, 鱼杨华1, 刘倩1, 张阳灿1, 李希来2,4, 寇建村1,2   

  1. 1. 西北农林科技大学草业与草原学院, 陕西 杨凌 712100;
    2. 三江源区高寒草地生态教育部重点实验室, 青海 西宁 810016;
    3. 西北农林科技大学生命科学学院, 陕西 杨凌 712100;
    4. 青海大学农牧学院, 青海 西宁 810016
  • 收稿日期:2024-05-15 修回日期:2024-07-13 发布日期:2025-03-01
  • 通讯作者: 寇建村,E-mail:jiancun02@163.com
  • 作者简介:武燕茹（1997-）,女,汉族,内蒙古乌兰察布人,硕士研究生,主要从事退化高寒草地修复研究,E-mail:wyr@nwafu.edu.cn
  • 基金资助:
    国家自然科学联合基金项目（U21A20191）;三江源区高寒草地生态教育部重点实验室项目（2023-SJY-KF-05）资助

Screening,Identification,and Growth Promoting Effects Analysis of A Highly Efficient Phosphorus Solubilizing Bacterium

WU Yan-ru1,2, YANG Wen-quan3, LIU Jia-qing1, KOU Wei-liang1, YU Yang-hua1, LIU Qian1, ZHANG Yang-can1, LI Xi-lai2,4, KOU Jian-cun1,2   

  1. 1. College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
    2. State Key Laboratory of Plateau ecology and Agriculture, Xining, Qinghai Province 810016, China;
    3. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
    4. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China
  • Received:2024-05-15 Revised:2024-07-13 Published:2025-03-01

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摘要： 为挖掘青藏高原特殊环境条件下牧草根际溶磷菌资源,促进退化高寒草地的治理,本研究从青海省门源县矮生嵩草（Kobresia humilis）根际土壤中分离到1株溶磷菌PSB,用16S rRNA基因分析法确定了其分类地位,并通过钼锑抗比色法和盆栽试验进行了溶磷与促生效果的研究。结果表明：菌株PSB为草假单胞菌（Pseudomonas poae）,可在10℃低温下正常生长。溶磷量高于700 μg·mL-1,可产多种有机酸,其中,草酸、乳酸、乙酸、柠檬酸、琥珀酸含量较高,总有机酸量为7461.88 μg·mL-1。同时,有产生长素（Indole-3-acetic acid,IAA）、ACC脱氨酶及铁载体的能力。盆栽试验结果表明,菌株PSB可以促进垂穗披碱草（Elymus nutans）生长,使植株高度增加16%,地上生物量增加12%,植株全磷含量增加10%；接种PSB对土壤全磷含量及有效磷含量均有显著的促进作用（P<0.05）,土壤有效磷从0.91 g·kg-1增加到1.97 g·kg-1。综上所述,菌株PSB具有较强的溶磷和促植物生长能力,是高寒地区生产微生物菌肥的优良菌种资源。

关键词: 高寒草甸, 根际促生菌, 溶磷菌, 促生效果

Abstract: In order to explore the resources of phosphorus solubilizing bacteria in the rhizosphere of forage under the special environmental conditions at the Qinghai Xizang Plateau to promote the management of alpine degraded grasslands. In this study,we isolated a phosphorus solubilizing bacterium PSB from the rhizosphere soil of Kobresia humilis in Menyuan County,Qinghai Province. It was classificated using 16S rRNA gene analysis. The phosphorus solubilizing and growth promoting effects were studied through the molybdenum antimony resistance colorimetry and pot experiments. The results showed that the strain PSB was belonged toPseudomonas poae,which can grow normally at a low temperature of 10℃. Its dissolved phosphorus content was higher than 700 μg·mL-1. It can produce the various organic acids with a total content of 7461.88 μg·mL-1. The cocntents of oxalic acid,lactic acid,acetic acid,citric acid,and succinic acid were high. It also had the ability to produce IAA （Indole-3-Acetic Acid）,ACC deaminase,and iron carrier. The results of the pot experiment showed that the strain PSB could promote the growth of Elymus nutans,with a 16% increase in plant height,a 12% increase in aboveground biomass,and a 10% increase in plant total phosphorus. The vaccination with PSB significantly promoted the contents of total phosphorus and available phosphorus in soil （P<0.05）. The available phosphorus in the soil increased from 0.91 g·kg-1 to 1.97 g·kg-1. Therefore,the strain PSB has strong ability to dissolve phosphorus and promote plant growth. It is an excellent strain resource for producing microbial fertilizers in high-altitude and cold regions.

Key words: Alpine meadows, Plant Growth Promoting Rhizobacteria, Phosphorus solubilizing bacteria, Promoting effect

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