草地学报

草地学报 ›› 2022, Vol. 30 ›› Issue (11): 2899-2909.DOI: 10.11733/j.issn.1007-0435.2022.11.007

• 研究论文 • 上一篇    

解淀粉芽孢杆菌DGL1促燕麦生长分子机制及代谢通路探究

杨雪1,2, 王添3, 谢永丽1,2,3, 乔有明2, 陈海龙3, 陈兰3, 武玲玲3   

  1. 1. 青海省青藏高原优良牧草种质资源利用重点实验室, 青海 西宁 810016;
    2. 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 青海 西宁 810016;
    3. 青海大学农牧学院, 青海 西宁 810016
  • 收稿日期:2022-05-11 修回日期:2022-06-16 发布日期:2022-12-02
  • 通讯作者: 谢永丽,E-mail:986237342@qq.com
  • 作者简介:杨雪(1996-)，女，青海西宁人，博士研究生，主要从事资源微生物的研究，E-mail:406448816@qq.com
  • 基金资助:
    国家自然科学基金项目(32160030)；青海省科技厅国际科技合作专项(2018-HZ-813)；青海大学省部共建三江源生态与高原农牧业国家重点实验室自主课题项目(2019-ZZ-12)；青海省科技厅项目(2017-ZJ-788)资助

Bacillus amyloliquefaciens DGL1 Promotes Avena sativa Growth and the Underlying Molecular Mechanism of Metabolic Pathways

YANG Xue1,2, WANG Tian3, XIE Yong-li1,2,3, QIAO You-ming2, CHEN Hai-long3, CHEN Lan3, WU Ling-ling3   

  1. 1. Key Laboratory of Use of Forage Germplasm Resources on Tibetan Plateau of Qinghai province, Xining, Qinghai Province 810016, China;
    2. State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Xining, Qinghai Province 810016, China;
    3. College of agriculture and animal husbandry, Qinghai university, Xining, Qinghai Province 810016, China
  • Received:2022-05-11 Revised:2022-06-16 Published:2022-12-02

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摘要： 分离自青海大格勒干旱沙地的解淀粉芽孢杆菌DGL1(Bacillus.amyloliquefaciens)被证实能够促进青海本地燕麦(Avena sativa)品种‘青燕1号’的生长。为了揭示DGL1促进燕麦生长的分子机制，本研究通过Illumina高通量转录组测序技术分析燕麦根部与菌株DGL1菌悬液分别互作2h，4h，8h，12h的处理组与对照组(CK)间的转录组差异表达基因，并通过GO富集、KEGG Pathway富集分析燕麦地上部分差异表达基因的相关代谢通路及关键基因。结果表明：燕麦根部与DGL1菌悬液互作2h，4h，8h和12h，燕麦叶部分别有1894，6130，8033和12215个差异表达基因，显著富集在氨基酸代谢、植物光合作用、次级产物代谢通路和与燕麦生长发育调控等相关代谢途径；通过KEGG富集分析发现IAA合成的色氨酸代谢途径中生长素早期响应蛋白编码基因AUXI、茉莉酸信号途径中核心受体编码基因COI1，铵转运蛋白编码基因AMT等基因显著上调，推测菌株DGL1对燕麦的促生机制是通过促进燕麦光合作用、激素代谢、次生代谢物合成、氨基酸代谢等多个途径相互协调的结果。

关键词: 解淀粉芽孢杆菌, 微生物-植物互作, 燕麦促生, 转录组测序, 差异表达基因分析

Abstract: The Bacillus amyloliquefaciens DGL1 was isolated from the dry sand soil of Dagele area in Qinghai Province,and has been shown to promote the growth of Qinghai native Avena sativa breed ‘Green Oats No.1’. In order to reveal the underlying molecular mechanism,in this study,we placed roots into a suspension of B. amyloliquefaciens DGL1 for 2 h,4 h,8 h and 12 h,and collected leaves and roots for Illumina high-throughput transcriptome sequencing on leaves and roots. The differentially expressed genes (DEGs) in leaves and roots between Avena sativa and the control (CK) were subjected to enrichment analysis of GO and KEGG,for discovering the relevant metabolic pathways. The results showed that 1 894,6 130,8 033 and 12 215 DEGs were significantly enriched in metabolic pathways of amino acid metabolism,plant photosynthesis,secondary metabolite and other ones related to growth and development of A. sativa. The KEGG enrichment analysis revealed a group of significantly up-regulated genes,including gene AUXI encoding IAA early responsive protein involved in the tryptophan metabolism pathway,gene COI1 encoding the core receptor involved in the jasmonic acid signaling pathway,and gene AMT encoding the ammonium transporter protein. It was hypothesized that the growth of A. sativa promoted by strain DGL1 is a result of the integration of multiple pathways,in terms of enhanced photosynthesis,hormone metabolism,secondary metabolite synthesis,and amino acid metabolism.

Key words: Bacillus amyloliquefaciens, Growth-promoting of Avena sativa, Plant-microbe interactions, Transcriptome sequencing, Differentially expressed gene analysis

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