草地学报

草地学报 ›› 2023, Vol. 31 ›› Issue (3): 719-725.DOI: 10.11733/j.issn.1007-0435.2023.03.011

• 研究论文 • 上一篇    下一篇

镉胁迫对绢毛委陵菜-丛枝菌真菌共生体超微结构和生理特性的影响

冯郑红1, 高宇2, 高冰1, 陈鹏1, 吴建慧1   

  1. 1. 东北林业大学园林学院, 黑龙江 哈尔滨 150040;
    2. 黑龙江省森林保护研究所, 黑龙江 哈尔滨 150040
  • 收稿日期:2022-07-04 修回日期:2022-12-08 出版日期:2023-03-15 发布日期:2023-04-01
  • 通讯作者: 陈鹏,E-mail:63411188@qq.com;吴建慧,E-mail:455259958@qq.com
  • 作者简介:冯郑红(1998-),女,汉族,山东省烟台人,主要从事园林植物逆境胁迫分子及生理研究,E-mail:1759600537@qq.com
  • 基金资助:
    中央高校基本科研业务基金项目（2572022BK02）资助；黑龙江省自然科学基金项目（LH2020C045）资助

Effects of Cadmium Stress on the Root Ultrastructure and Physiology of Potentilla sericea-Arbuscular Mycorrhizal Fungi Symbiont

FENG Zheng-hong1, GAO Yu2, GAO Bing1, CHEN Peng1, WU Jian-hui1   

  1. 1. College of Landscape Architecture, Northeast Forestry University, Harbin, Heilongjiang Province 150040, China;
    2. Forest Protection Research Institute, Harbin, Heilongjiang Province 150040, China
  • Received:2022-07-04 Revised:2022-12-08 Online:2023-03-15 Published:2023-04-01

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摘要： 为探究绢毛委陵菜（Potentilla sericea）-丛枝菌根真菌（Arbuscular mycorrhiza fungi，AMF）共生体对重金属胁迫的应答机制，了解绢毛委陵菜抗镉机理，本研究以绢毛委陵菜与根内根生囊霉（Rhizophagus intraradices）为试验材料，以栽植在未接种根内根生囊霉的灭菌土壤绢毛委陵菜为对照组，以栽植在接种根内根生囊霉的灭菌土壤绢毛委陵菜为处理组，用2.5 CdCl2·H2O处理并测定菌根侵染率，共生体植株的结构变化和生理指标变化。结果表明：根内根生囊霉对绢毛委陵菜菌根侵染率为89.33%；根内根生囊霉会减轻镉胁迫下绢毛委陵菜超微结构的损伤；与未接根内根生囊霉的绢毛委陵菜相比，绢毛委陵菜-根内根囊霉共生体在镉胁迫条件下，SOD和POD活性升高、可溶性蛋白含量升高、MDA含量降低。因此，丛枝菌真菌能够有效减轻重金属胁迫对绢毛委陵菜的伤害，能够利用接种AMF提高绢毛委陵菜在重金属胁迫下抗性。

关键词: 绢毛委陵菜, 镉胁迫, 丛枝菌真菌, 生理指标, 超微结构

Abstract: In order to explore the mechanism of response and resistance of Potentilla sericea Arbuscular mycorrhiza fungi symbiont to Cadmium stress, P. sericea and Rhizophagus intraradices were used as experiment materials in this study. P. sericea planted in the sterilized soil uninoculated with Rhizophagus was used as the control group and P. sericea planted in the sterilized soil inoculated with Rhizophagus was used as the treatment group. The experimental group and control group were treated with 2.5 CdCl2·H2O and determined the mycorrhizal infection rate, structural changes, and symbiont plants' physiological traits. The main research results are as follows:the root infection rate of Rhizophagus intraradices was as high as 89.33%;Rhizophagus alleviated the ultrastructure damage of P. sericata under Cadmium stress;compared to P. sericea without Arbuscular mycorrhizal fungi (AMF), the content of MDA of P. sericea AMF symbiont decreased, the activities of SOD and POD increased and the content of soluble protein increased under Cadmium stress. The AMF can effectively reduce the damage of P. sericea from heavy metal stress. Thus the AMF inoculation could be used to improve the resistance to heavy metal stress.

Key words: Potentilla sericea, Cadmium stress, Arbuscular mycorrhizal fungi, Physiological traits, Ultrastructure

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