“中国春”小麦-偃麦草易位系的创制与鉴定

doi:10.11733/j.issn.1007-0435.2009.04.008

›› 2009, Vol. 17 ›› Issue (4): 452-455.DOI: 10.11733/j.issn.1007-0435.2009.04.008

“中国春”小麦-偃麦草易位系的创制与鉴定

刘凤歧¹, 张月学¹, 唐凤兰¹, 李集临², 徐香玲²

1. 黑龙江省农业科学院草业研究所, 黑龙江, 哈尔滨, 150086;
2. 哈尔滨师范大学生命与环境科学学院生物系, 黑龙江, 哈尔滨, 150025

收稿日期:2009-02-18 修回日期:2009-06-12 出版日期:2009-08-15 发布日期:2009-08-15
作者简介:刘凤歧(1982- ),男,黑龙江克山人,硕士,实习研究员,从事牧草细胞遗传学与育种研究,E-mail:l_fq2003@163.com
基金资助:
国家自然科学基金资助项目(No.39870417)

Creation and Identification of ‘Chinese Spring’ wheat Elytrigia elongata Translocation Lines

Liu Feng-qi¹, ZHANG Yue-xue¹, TANG Feng-lan¹, LI Ji-lin², XU Xiangling²

1. Institute of Pratacultural Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China;
2. Biology Department, Life and Environment Science College, Harbin Normal University, Harbin, Heilongjiang Province 150080, China

Received:2009-02-18 Revised:2009-06-12 Online:2009-08-15 Published:2009-08-15

摘要/Abstract

摘要： 利用杀配子染色体创制易位系的方法,以"中国春"小麦(Triticum aestivum L.)-长穗偃麦草(Elytrigia repens)(2E)异二体附加系为母本与"中国春"小麦-柱穗山羊草(Ae.cylindrical)(2C)异二体附加系为父本杂交,创制"中国春"小麦-偃麦草易位系.F1的结实率为31.02%,F1套袋自交结实率为31.41%,亲本自交结实率92.59%;获得的F2利用染色体C-分带和原位杂交进行鉴定,从127株后代中筛选出9株易位系.

关键词: 长穗偃麦草, 杀配子染色体, C-分带, 原位杂交, 易位系

Abstract: The ‘Chinese Spring’ wheat (Triticum aestivum L.)-Elytrigia elongata translocation lines were created by hybridizing ‘Chinese Spring’ wheat-Elytrigia elongata 2E disomic addition line with,the method of gametocidal chromosome creating translocation line,the ‘Chinese Spring’ wheat-Ae. cylindrical 2C disomic addition line.The F₁ generation had setting percentage of 31.02%,and it's inbreeding rate was 31.41%, which was much lower than 92.59% of the parental lines.9 inter-genomic translocations,involving Chinese Spring and E.elongata chromosomes,were screened out from 127F₂ progenies by C-banding and genomic in situ hybridization (GISH).

Key words: E.elongata,Gametocidal chromosome, C-banding,In situ hybridization,Translocation lines

中图分类号:

S330.25

刘凤歧, 张月学, 唐凤兰, 李集临, 徐香玲. “中国春”小麦-偃麦草易位系的创制与鉴定[J]. , 2009, 17(4): 452-455.

Liu Feng-qi, ZHANG Yue-xue, TANG Feng-lan, LI Ji-lin, XU Xiangling. Creation and Identification of ‘Chinese Spring’ wheat Elytrigia elongata Translocation Lines[J]. , 2009, 17(4): 452-455.

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“中国春”小麦-偃麦草易位系的创制与鉴定

Creation and Identification of ‘Chinese Spring’ wheat Elytrigia elongata Translocation Lines

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