草地学报

›› 2009, Vol. 17 ›› Issue (3): 349-353.DOI: 10.11733/j.issn.1007-0435.2009.03.016

• 研究论文 • 上一篇    下一篇

中间偃麦草、长穗偃麦草及其杂种F1代同工酶研究

撒多文, 米福贵, 刘玉良   

  1. 内蒙古农业大学生态环境学院, 呼和浩特, 010019
  • 收稿日期:2008-12-30 修回日期:2009-03-27 出版日期:2009-06-15 发布日期:2009-06-15
  • 通讯作者: 米福贵,E-mail:mfgui@yahoo.com.cn
  • 作者简介:撒多文(1980- ),男,汉,陕西宝鸡人,硕士研究生,研究方向为牧草遗传育种与生物技术,E-mail:saduowen0225@163.com
  • 基金资助:
    国家“十一五”科技支撑计划(2008BADB3B00)

Isozyme Studies of Elytrigia Hybrids(E. elongate×E. intermedia) and Their Parents

SA Duo-wen, MI Fu-gui, LIU Yu-liang   

  1. College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010019, China
  • Received:2008-12-30 Revised:2009-03-27 Online:2009-06-15 Published:2009-06-15

PDF

摘要： 通过对过氧化物酶(POD)、酯酶(EST)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)同工酶谱的分析,探讨中间偃麦草(Elytrigia elongata(Host)Nevski)、长穗偃麦草(E.intermedia(Host)Nevski)及其杂种F1代的酶谱特征和亲缘关系。结果表明:亲本同工酶酶带数目、迁移率、着色程度等差异不大,二者亲缘关系较近。与亲本相比,杂种F1代存在POD、SOD、PPO酶带丢失现象,但正、反交植株中均产生了一些亲本没有的新生带。杂种F1代EST酶谱与亲本基本相同,仅着色程度略有差异。杂种F1代植株的POD、PPO酶谱特征有偏向中间偃麦草的倾向,而SOD酶谱特征倾向于长穗偃麦草。杂种F1代同工酶具有多态性,可作为遗传标记用于杂种鉴定和后代株系目标性状的选择。

关键词: 中间偃麦草, 长穗偃麦草, 同工酶

Abstract: Through the analysis of isozyme zymograms of peroxidase(POD),esterase(EST),superoxide dismutase(SOD),and polyphenol oxidase(PPO),the zymogram characteristics and relationship of Elytrigia hybrids,E. elongate×E. intermedia,and their parents were studied.The results are as follows: the band number,migration rate,and pigmentation degree of isoenzymes between parents had slight differences and their genetic relationship could be close.F1 hybrids lacked of some bands of POD,SOD,and PPO isoenzyme as compared to their parents,but had some new bands in descendant of reciprocal cross;EST bands of hybrid F1 were almost the same as the parents except the pigmentation degree.The characteristic of isozyme bands of POD and PPO of hybrid F1 had the tendency to E.elongata,and the SOD isozyme band to E.intermedia.Isozyme Polymorphisms existed in hybrid F1 which could be used as the genetic marker to identify hybrids and determine the plants with target characteristics in descendant strains.

Key words: Elytrigia elongata(Host) Nevski, E.intermedia(Host) Nevski, Isoenzyme

中图分类号: