草地学报 ›› 2026, Vol. 34 ›› Issue (5): 1677-1690.DOI: 10.11733/j.issn.1007-0435.2026.05.011

• 研究论文 • 上一篇    

无芒雀麦种质材料萌发期耐旱性综合评价

黄梦琪1, 李陈建1, 王玉祥1, 路璐1, 王清屿1, 蒋优1, 童爱香2   

  1. 1. 西部干旱荒漠区草地资源与生态教育部重点实验室, 新疆草地资源与生态重点实验室, 新疆农业大学草业学院, 新疆 乌鲁木齐 830052;
    2. 北京市科学技术研究院, 北京 100093
  • 收稿日期:2025-08-22 修回日期:2025-11-09 发布日期:2026-05-08
  • 通讯作者: 王玉祥,E-mail:wyx9868@163.com
  • 作者简介:黄梦琪(2000-),女,汉族,新疆阜康人,硕士研究生,主要从事草种质资源研究与育种相关的研究,E-mail:2822557516@qq.com;
  • 基金资助:
    财政部和农业农村部:国家现代农业产业技术体系牧草产业技术体系(CARS-34),新疆维吾尔自治区饲草产业技术体系(XJARS-13-04),第九师草原野生优良牧草选育研究项目(2023JS006)共同资助

Comprehensive Evaluation of Drought Tolerance in Bromus inermis Germplasm at the Germination Stage

HUANG Meng-qi1, LI Chen-jian1, WANG Yu-xiang1, LU Lu1, WANG Qing-yu1, JIANG Yiu1, TONG Ai-xiang2   

  1. 1. Key Laboratory of Grassland Resources and Ecology of Western Arid Region Ministry of Education, Key Laboratory of Grassland Resources and Ecology of Xinjiang, College of Grassland Science Xinjiang Agricultural University, Urumqi, Xinjiang Province 830052, China;
    2. Beijing Academy of Science and Technology, Beijing 10093, China
  • Received:2025-08-22 Revised:2025-11-09 Published:2026-05-08

摘要: 干旱是影响植物生产的主要胁迫因子之一,萌发期是植物生命周期中最脆弱的时期。在无芒雀麦(Bromus inermis)耐旱性育种中,萌发期耐旱评价尤为重要。本研究采取双层滤纸发芽法,通过测定25% PEG-6000胁迫下的100份无芒雀麦种质资源的发芽率、发芽势、发芽指数、根长、芽长等8个指标,利用主成分分析、隶属函数和系统聚类分析法,对其萌发期耐旱性进行综合评价。结果表明:与对照相比,25% PEG胁迫下各观测指标均有下降,变异系数范围为20.853%~97.876%;多数指标之间存在显著性相关(P<0.05);不同材料的耐旱性存在较大差异,综合耐旱系数在0.612~0.169之间,D值在0.861~0.068之间;聚类分析将100份无芒雀麦种质资源分为4类,其中高度耐旱性14份,中度耐旱性34份,低度耐旱性45份和干旱敏感性7份。本研究筛选出耐旱性强的种质包括W1Z622,W1Z627,W1Z595等,为无芒雀麦耐旱育种提供优质材料和理论依据。

关键词: 无芒雀麦, 种子萌发, 耐旱性, 综合评价, 聚类分析

Abstract: Drought is one of the primary stress factors affecting plant production, and the germination stage is the most vulnerable period in the plant life cycle. In the breeding of drought-resistant Bromus inermis, evaluating drought tolerance during the germination stage is particularly important. This study employed the double-layer filter paper germination method, measuring eight indicators-including germination rate, germination potential, germination index, root length, and shoot length-under 25% PEG-6000 stress for 100 B. inermis germplasm resources. Principal component analysis, membership functions, and hierarchical clustering analysis were used to comprehensively assess drought tolerance during the germination stage. The results showed that, compared to the control, all observed indicators decreased under 25% PEG stress, with coefficient of variation ranging from 20.853% to 97.876%. There was a significant correlation (P<0.05) among most experimental indicators. There were substantial differences in drought tolerance among materials, with the comprehensive drought tolerance coefficient ranging from 0.612 to 0.169 and the D-value ranging from 0.861 to 0.068. Cluster analysis classified the 100 t100 Bromus inermis germplasm resources into four groups: highly drought-resistant (14 materials), moderately drought-resistant (34 materials), weakly drought-resistant (45 materials), and drought-sensitive (7 materials). This study identified drought-resistant germplasm such as W1Z622, W1Z627, and W1Z595. The research provides high-quality materials and theoretical support for drought-resistant breeding of B. inermis.

Key words: Bromus inermis, Seed germination, Drought tolerance, Comprehensive evaluation, Cluster analysis

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