Acta Agrestia Sinica

›› 2013, Vol. 21 ›› Issue (3): 607-611.DOI: 10.11733/j.issn.1007-0435.2013.03.029

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Optimization of Alfalfa Rhizobium Medium

ZHOU Ji-qiong1, DENG Bo1, MA Xiao-tong2, ZHANG Ying-jun1, ZHOU Ke1   

  1. 1. Department of Grassland Science, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China;
    2. Soil and Fertilizer Institute, Chinese Academy of Agricultural Science, Beijing 100081, China
  • Received:2012-12-13 Revised:2013-03-11 Online:2013-06-15 Published:2013-06-05

紫花苜蓿根瘤菌培养基的优化研究

周冀琼1, 邓波1, 马晓彤2, 张英俊1, 周可1   

  1. 1. 中国农业大学动物科技学院草业科学系, 北京 100193;
    2. 中国农业科学院土肥所, 北京 100081
  • 通讯作者: 邓波
  • 作者简介:周冀琼(1987- ),女,甘肃武威人,硕士研究生,研究方向为草地资源、生态与管理,E-mail:qiongqionghh221@163.com
  • 基金资助:
    国家牧草产业技术体系(CARS-35);公益性行业(农业)科研专项(201003061);"十二五"农村领域国家科技支撑计划课题(2012BAD13B07)资助

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Abstract: The optimum media and formulas of representative strains Sinorhizobium meliloti ACCC17631 and ACCC17676 were confirmed by single factor carbon source and nitrogen source utilization experiment and orthogonal design experiment. Results showed that the optimum carbon source was sucrose,and the optimum nitrogen source was yeast extract and soybean meal. The optimum medium formulas of ACCC17631 (ACCC17676) was (g 稬-1):sucrose 20 + soybean meal (yeast extract) 2.4+K2HPO4 0.25+KH2PO4 0.25+MgSO4?7H2O 0.2+NaCl 0.1, and pH 6.8~7.0.

Key words: Alfalfa, Rhizobium, Medium, Optimization

摘要： 以苜蓿根瘤菌(Sinorhizobium meliloti)ACCC17631和ACCC17676为供试菌株,采用单因素碳氮源利用试验和正交设计试验,确定最佳碳源、氮源及培养基配方。结果表明:2株供试菌株最佳碳源为蔗糖,最佳氮源分别为大豆粉和酵母粉。根瘤菌ACCC17631(ACCC17676)的最佳培养基配方(g ·L-1)为:蔗糖 20,大豆粉(酵母粉)2.4, K2HPO4 0.25, KH2PO4 0.25, MgSO4·7H2O 0.2, NaCl 0.1, pH为6.8~7.0。

关键词: 紫花苜蓿, 根瘤菌, 培养基, 优化

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