›› 2013, Vol. 21 ›› Issue (6): 1113-1118.DOI: 10.11733/j.issn.1007-0435.2013.06.012

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Stress Resistence of Alfalfa Rhizobium from Different Soil Types

ZHU Rui-fen, TANG Feng-lan, LIU Jie-lin, LIU Feng-qi, CHEN Ji-shan   

  1. Institute of Pratacultural Science, Heilongjiang Academy of Agricultural Science, Harbin, Heilongjiang Province 150086, China
  • Received:2013-04-07 Revised:2013-06-25 Online:2013-12-15 Published:2013-11-27

不同土壤中紫花苜蓿根瘤菌抗逆性的比较

朱瑞芬, 唐凤兰, 刘杰淋, 刘凤歧, 陈积山   

  1. 黑龙江省农科院草业研究所, 黑龙江 哈尔滨 150086
  • 通讯作者: 陈积山
  • 作者简介:朱瑞芬(1982-),女,甘肃陇南人,硕士,研究方向为植物根际促生菌,E-mail:zhuruifeng1983@aliyun.com
  • 基金资助:
    黑龙江省农业科技创新工程2012年度院级科研项目(2012QN001)资助

Abstract: Six rhizobia strains were isolated from the alfalfa root of two soil types in Heilongjiang province. The salt-resistant, acid-alkali resistant and acid-alkali secreting ability of each strain were studied using GGE method. Result showed that alfalfa rhizobia strain numbered as Hmx-c from black soil type had the strongest salt-resistant ability, and regularly grew in 10% NaCl medium. Alfalfa rhizobia strain numbered as Lmx-a from saline-alkali soil type and Hmx-b from black soil type had the strongest acid-resistant ability, and regularly grew in medium with pH value of 4.0. The strain numbered as Hmx-c from saline-alkali soil type possessed the strongest alkali-resistant ability, and regularly grew in medium with pH value of 12.0. A further experiment indicated that strain numbered as Hmx-a resisted both acid stress with pH value of 4.0 and alkali stress with pH value of 11.0. Meanwhile, the ability of alkali resistance was interrelated to the ability of acid secreting.

Key words: Rhizobia, Saline tolerance, Acid resistance and alkali tolerance

摘要: 为准确评价紫花苜蓿(Medicago sativa L.)根瘤菌的抗盐性,采用 GGE 双标图对来自盐碱土和黑土的6个紫花苜蓿根瘤菌(Rhizobium)菌株分别进行耐盐性、耐酸碱及其泌酸碱能力的评价研究。结果表明:黑土土壤紫花苜蓿根瘤菌株Hmx-c耐盐性极强,在10% NaCl培养基上生长正常;盐碱土壤紫花苜蓿根瘤菌株Lmx-a和黑土土壤紫花苜蓿根瘤菌株Hmx-b耐酸性最强,能在pH 4.0的培养基上正常生长;耐碱性最强的是盐碱土壤菌株Hmx-c,可以在pH 12.0的培养基上正常生长。进一步试验表明,菌株Hmx-a既能抗pH 4.0的酸逆境,又能抗pH 11.0的碱逆境。根瘤菌菌株耐碱性的强弱与其泌酸能力相关。

关键词: 根瘤菌, 耐盐性, 耐酸碱性

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