Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2018, Vol. 26 ›› Issue (2): 453-458.DOI: 10.11733/j.issn.1007-0435.2018.02.026

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The Effects of Cellulase on Silage Quality of Rice Straw with Normal and Low Moisture Contents

CHEN Xin-zhu1,2,3, ZOU Chang-lian1,4, ZHANG Wen-chang1, ZHUANG Yi-fen1, LIU Jing2   

  1. 1. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China;
    2. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian Province 350013, China;
    3. Fujian Minke Bio-technology Co. Ltd., Fuzhou, Fujian Province 350008, China;
    4. Fujian Aonong Bio-technology Group Co. Ltd., Zhangzhou, Fujian Province 363002, China
  • Received:2018-01-29 Revised:2018-05-04 Online:2018-04-15 Published:2018-07-05

纤维素酶对常规水分和低水分稻草青贮品质的影响

陈鑫珠1,2,3, 邹长连1,4, 张文昌1, 庄益芬1, 刘景2   

  1. 1. 福建农林大学动物科学学院, 福建 福州 350002;
    2. 福建省农业科学院畜牧兽医研究所, 福建 福州 350013;
    3. 福建省新闽科生物科技开发有限公司, 福建 福州 350008;
    4. 福建傲农生物科技集团股份有限公司, 福建 漳州 363002
  • 通讯作者: 庄益芬, 刘景
  • 作者简介:陈鑫珠(1985-),女,福建龙岩人,博士,助理研究员,主要从事饲料加工与贮藏研究,E-mail:010622051@163.com;邹长连(1988-),男,江西赣州人,硕士,主要从事动物营养与饲料的研究,E-mail:changlianz@126.com
  • 基金资助:

    福建省农业科学院青年英才计划（YC2016-9）；福建省教育厅科技项目（JK2009010）资助

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Abstract:

This experiment was carried out to evaluate the effects of adding cellulase on the quality ofrice straw silage. Normal moisture content (70%) (MC1) and low moisture content (37.5%) (MC2) rice straw were ensiled in laboratory silos after addition of cellulase at rates of 0, 20, 40 and 80 mg·kg-1 fresh straw (short for CK, CEL1, CEL2 and CEL3). After 60 days of storage, the silos were opened for chemical analyses. The results showed that:Compared to MC1silages, the MC2 silages had higher pH value and dry matter recovery (DMR) (P<0.05) and lower NH3-N content (P<0.05). The water soluble-carbohydrate (WSC) content was significantly increased (P<0.05) and the contents of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were significantly decreased (P<0.05) in MC1 silages, and the pH value and NDF content were significantly decreased (P<0.05) and dry matter (DM) content was significantly increased (P<0.05) in MC2 silages when treated with cellulase. The pH value was significantly decreased by CEL1 and CEL2 treatments (P<0.05) in MC1 silages, the DMR silages was significantly increased by CEL2 and CEL3 treatments (P<0.05) and the WSC content was significantly increased by CEL2 treatment (P<0.05) in MC2 silages. The CEL2 treatment had highest DM content, DMR and WSC content, and lowest pH value, GLR, NDF content, ADF content and NH3-N content. This study confirmed that the fermented quality of silage were improved when cellulase was added to MC1 and MC2 silages and the 40 mg·kg-1 treatment had the best effects.

Key words: Rice straw, Cellulase, Silage, Fermentation quality, Chemical component

摘要：

为了研究不同水平纤维素酶对常规和低水分稻草青贮发酵品质的影响，为生产优质稻草青贮饲料提供理论依据。试验调制常规水分（70.00%）和低水分（37.50%）两种水分的稻草青贮，每种水分均设4组纤维素酶（cellulase，CEL）添加浓度，分别为0（CON）、20（CEL1）、40（CEL2）和80 mg·kg-1（CEL3），共计8个处理。每个处理重复3次。常温下青贮60 d开封，测定青贮饲料的发酵品质和化学成分。结果发现：两种水分青贮料中，低水分青贮料的pH和DMR显著（P<0.05）提高，NH3-N含量显著（P<0.05）低于常规水分青贮料；常规青贮中，4组纤维素酶浓度组的WSC含量显著（P<0.05）增加、NDF和ADF含量显著（P<0.05）减少，其中CEL1组和CEL2组的pH显著（P<0.05）降低；低水分青贮中，4组纤维素酶浓度组的pH和NDF含量显著（P<0.05）降低、DM含量显著（P<0.05）增加，另外，CEL2组和CEL3组的DMR含量显著（P<0.05）提高，CEL2组的WSC含量显著（P<0.05）增加；2种水分青贮中，均以CEL2组的DM、DMR、WSC最高，pH、GLR、NDF含量、ADF含量、NH3-N含量最低。研究结果表明，在常规水分和低水分稻草青贮中，3个纤维素酶水平都有显著的添加效果，且以40mg·kg-1的添加效果最佳。

关键词: 稻草, 纤维素酶, 低水分青贮, 发酵品质, 化学成分

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