Acta Agrestia Sinica ›› 2018, Vol. 26 ›› Issue (3): 611-617.DOI: 10.11733/j.issn.1007-0435.2018.03.012

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GC-MS Analysis of Root Exudates of Alfalfa In Different Cropping Years on The Loess Plateau

BI Bo-yuan1, HAN Feng-peng1,2   

  1. 1. College of Resources and Environment, Northwest A & F University, Yangling, Shaannxi Province 712100, China;
    2. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A & F University, Yangling, Shaannxi Province 712100, China
  • Received:2017-09-29 Revised:2018-05-14 Online:2018-06-15 Published:2018-08-24

黄土高原不同种植年限紫花苜蓿根系分泌物GC-MS分析

毕博远1, 韩凤朋1,2   

  1. 1. 西北农林科技大学 资源环境学院, 陕西 杨凌 712100;
    2. 西北农林科技大学黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西 杨凌 712100
  • 通讯作者: 韩凤朋
  • 作者简介:毕博远(1992-),男,陕西西安人,硕士研究生,主要从事土壤环境化学研究,E-mail:524300838@qq.com
  • 基金资助:

    国家自然科学基金项目(41471437);国家重点研发计划(2016YFA0600801;2017YFC0504504);科技基础性工作专项项目(2014FY210100);中国科学院科技服务网络计划(KFJ-STS-ZDTP-012);中国科学院“西部青年学者”项目(XAB2016A04)资助

Abstract:

In order to study the components of ‘ZhongMu No.1’ alfalfa root exudates in different planting years (1 year, 10 years, 20 years, 30 years), we leached its rhizosphere soil with two different polar solvents (ethyl acetate, dichloromethane). Finally, the chemical components of root exudates were analyzed by GC-MS. The results showed that:the root exudates of alfalfa mainly contained alkanes, esters, alcohols, acids, phenols, ketones and ethers, among which the proportion of alkanes was the largest, accounting for more than 75%. Most of the alkanes are linear, and a small number of them are branched alkanes and naphthenes. In the results of dichloromethane extraction, octadecane, hexadecane and tetradecane accounted for a larger proportion of planting 10 years, 20 years, 30 years of alfalfa root exudates. Diethyl phthalate, 1.54-dibromo pentadecane, eicosane, hexacosane and octadecane accounted for a larger proportion of the 1-year-planting alfalfa exudates. The results of ethyl acetate extraction showed that the relative content of octadecane was 8.82%, 11.5%, 17.85% and 25.69% respectively in the four kinds of cropping years. It extracted a relatively small amount of material compared with the results of methylene chloride.

Key words: Different planting years, Root exudates, GC-MS, Heneicosane

摘要:

为研究不同种植年限(1年、10年、20年、30年)紫花苜蓿‘中苜一号’根系分泌物的组分,分别以不同极性的浸提剂(乙酸乙酯、二氯甲烷)浸提其根际土壤,最终借助GC-MS气质联用技术分析其根系分泌物的化学组分。结果表明:苜蓿的根系分泌物主要含有烷烃类、酯类、醇类、酸类、酚类、酮类、醚类化合物,其中以烷烃类所占比重最大,占到75%以上,多数为直链烷烃,但也包括少数的支链烷烃和环烷烃。在二氯甲烷提取结果中种植10年、20年、30年的苜蓿根系分泌物以二十一烷、三十六烷、四十烷所占的比例较大,在种植1年的苜蓿分泌物中又以邻苯二甲酸二乙酯、1.54-二溴五十四烷、二十烷、三十六烷、二十一烷等所占比例较大。乙酸乙酯提取结果显示,在四种年限的提取结果中二十一烷的相对含量比例较大,分别占8.82%、11.5%、17.85%、25.69%,但相较于二氯甲烷结果,它提取到的物质种类相对较少。

关键词: 不同种植年限, 根系分泌物, GC-MS, 二十一烷

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