›› 2010, Vol. 18 ›› Issue (6): 823-828.DOI: 10.11733/j.issn.1007-0435.2010.06.013

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Effects of NaCl and Na2SO4 Stress on the Content of Na+,K+ and Plasma Membrane H+-ATPase Activity of Achnatherumsplendens Seedling

DONG Qiu-li, XIA Fang-shan, DONG Kuan-hu   

  1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China
  • Received:2010-04-15 Revised:2010-10-18 Online:2010-12-15 Published:2010-12-15

盐胁迫对芨芨草种子萌发苗Na+,K+含量与质膜H+-ATPase活性的影响

董秋丽, 夏方山, 董宽虎   

  1. 山西农业大学动物科技学院, 山西, 太谷, 030801
  • 通讯作者: 董宽虎,E-mail:dongkuanhu@126.com
  • 作者简介:董秋丽(1985- ),女,山西阳泉人,硕士研究生,研究方向为牧草抗逆性研究,E-mail:d394875786@126.com
  • 基金资助:
    “十一五”国家科技支撑计划课题(2007BAD56B01);农业部公益性行业(农业)科研专项(nyhyzx07-022);山西省科技攻关项目(20080312002-1)资助

Abstract: The content of Na+,K+ and Plasma membrane H+-ATPase activity in leaves and roots of Achnatherumsplendens were determined in order to investigate the distribution of Na+,K+ and the plasma membrane H+-ATPase activity on Achnatherumsplendens under varying levels of NaCl and Na2SO4.Results show that with the concentration of NaCl and Na2SO4 increasing,the content of Na+ increased and K+ decreased,then K+/Na+ ratio decreased in roots and leaves of seedlings in Achnatherumsplendens.The PM H+-ATPase activity increased only in roots of Achnatherumsplendens.The content of Na+ in leaves was lower than that in roots,and the content of K+ in leaves was higher than that in roots under NaCl and Na2SO4 stress.The K+/Na+ ratio in leaves was more than 1 and higher than that in roots.The PM H+-ATPase activity in roots was higher than that in leaves.The TSK,Na was higher under Na2SO4 stress than NaCl stress.The PM H+-ATPase activity in leaves and roots was higher under Na2SO4 stress than the same concentration of NaCl stress.Achnatherumsplendens was more tolerant to Na2SO4 stress than NaCl stress.

Key words: Salt tolerance, Achnatherumsplendens, Na+, K+, TSK,Na, PM H+-ATPase

摘要: 以芨芨草(Achnatherumsplendens)种子萌发苗为试验材料,分别以不同浓度NaCl和Na2SO4进行胁迫,通过对芨芨草叶片和根系中Na+,K+含量以及质膜H+-ATPase活性进行测定,以探讨盐胁迫对芨芨草中Na+,K+分布以及质膜H+-ATPase活性的影响。结果表明:随着NaCl和Na2SO4浓度的增加,芨芨草根系和叶片的Na+含量增加,K+含量下降,K+/Na+比值下降,根系中质膜H+-ATPase活性增加;在NaCl和Na2SO4胁迫下,芨芨草叶片中Na+含量显著低于根系,K+含量显著高于根系,叶片的K+/Na+比值均大于1并明显高于根系,根系的质膜H+-ATPase活性显著高于叶片;与NaCl相比,Na2SO4胁迫下,芨芨草根系向叶片的离子选择性运输系数(TSK,Na)较高,叶片和根系的质膜H+-ATPase活性明显高于相同浓度的NaCl胁迫组。与NaCl胁迫相比,芨芨草对Na2SO4胁迫的适应性更强。

关键词: 耐盐性, 芨芨草, Na+, K+, TSK,Na, 质膜H+-ATPase

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