Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (1): 112-122.DOI: 10.11733/j.issn.1007-0435.2019.01.015

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Germination Characteristics of Halogeton glomeratus under Different Heavy Metals Treatments with NaCl Stress

HOU Jing-jing1,2, LI Bao-chun1,3, WANG Jun-cheng1,2, YAO Li-rong1,2, BIAN Xiu-xiu1, HU Na1,2, SI Er-jing1,2, YANG Ke1,2, MENG Ya-xiong1,2, Ma Xiao-le1,2, WANG Hua-jun1,2   

  1. 1. Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu Province 730070, China;
    2. College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
    3. College of life Sciences and Technology, Gansu Agriculture University, Lanzhou, Gansu Province 730070, China
  • Received:2018-11-19 Revised:2019-03-04 Online:2019-02-15 Published:2019-04-13

NaCl胁迫下盐生草在不同重金属处理下的萌发特性分析

侯静静1,2, 李葆春1,3, 汪军成1,2, 姚立蓉1,2, 边秀秀1, 胡娜1,2, 司二静1,2, 杨轲1,2, 孟亚雄1,2, 马小乐1,2, 王化俊1,2   

  1. 1. 甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室, 甘肃 兰州 730070;
    2. 甘肃农业大学农学院, 甘肃 兰州 730070;
    3. 甘肃农业大学生命科学技术学院, 甘肃 兰州 730070
  • 通讯作者: 王化俊,E-mail:huajunwang@sina.com
  • 作者简介:侯静静(1994-),女,甘肃兰州人,硕士研究生,主要从事作物遗传育种研究,E-mail:528725294@qq.com;李葆春(1971-),女,甘肃陇南人,副教授,博士,主要从事小麦遗传育种研究,E-mail:libc@gsau.edu.cn
  • 基金资助:
    国家大麦青稞产业技术体系(CARS-05-03B-03);甘肃省重大专项(17ZD2NA016);甘肃农业大学学科建设专项基金(GAU-XKJS-2018-082、083);甘肃农业大学盛彤笙创新基金(GSAU-STS-2018-33)资助

Abstract: This study firstly researched germination characteristics of Halogeton glomeratus under different concentrations of heavy metals Cu2+,Zn2+,Ni2+,Cd2+ and Pb2+ supplemented with 100 mmol·L-1 NaCl. The results showed that germination rate and germination potential increased at first and then decreased with the increase of the heavy metals concentrations. It indicated that the low concentration of heavy metal ions promoted the germination of plants. Fresh weight,dry weight and seedlings height decreased gradually. The results of ion contents showed that Cd2+ and Pb2+ contents increased at first and then decreased,and Cu2+,Zn2+ and Ni2+ contents increased with increasing heavy metals concentrations. The root activity of Cu2+ and Zn2+ increased first and then decreased,and it decreased with the increase of Ni2+,Cd2+ and Pb2+ concentrations. The comprehensive clustering and principal component analysis were carried out to obtain the critical concentrations of Cu2+,Zn2+,Ni2+,Cd2+ and Pb2+ in Halogeton glomeratus,which were 1.00 mmol·L-1,10.00 mmol·L-1,0.30 mmol·L-1,0.20 mmol·L-1 and 0.50 mmol·L-1,respectively. The most important indexes were germination index,dry weight,germination energy,dry weight and germination energy when the stress concentration reached the critical value for Halogeton glomeratus.

Key words: Heavymetals, Stress, Halogeton glomeratus, Germination

摘要: 本研究首次以盐生草(Halogeton glomeratus)为材料,模拟盐胁迫(100 mmol·L-1 NaCl)环境,探讨不同浓度重金属Cu2+,Zn2+,Ni2+,Cd2+,Pb2+处理对其萌发特性的影响。结果表明:随处理浓度的升高,发芽率和发芽势均表现为先升高后下降趋势,表明低浓度重金属离子可促进植物的萌发,幼苗鲜重、干重、株高均呈现逐渐下降趋势。测定离子含量得出,Cd2+和Pb2+离子含量随着胁迫加重呈先升高后下降的趋势,Cu2+,Zn2+和Ni2+离子含量逐渐增加。测定根系活力发现,Cu2+,Zn2+根系活力随浓度的升高呈先上升后下降趋势,Ni2+,Cd2+,Pb2+根系活力逐渐降低。综合聚类及主成分分析得出:盐生草耐Cu2+,Zn2+,Ni2+,Cd2+,Pb2+的临界浓度分别为:1.00 mmol·L-1,10.00 mmol·L-1,0.30 mmol·L-1,0.20 mmol·L-1和0.50 mmol·L-1,在胁迫浓度达临界值时贡献率最大的指标分别为:萌发指标、干重、发芽势、干重和发芽势。

关键词: 重金属, 胁迫, 盐生草, 萌发

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