Effect of Barley Yellow Dwarf Virus Infection on Photosynthesis and Chlorophyll Fluorescence Parameters of Oat

doi:10.11733/j.issn.1007-0435.2020.04.008

Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (4): 923-931.DOI: 10.11733/j.issn.1007-0435.2020.04.008

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Effect of Barley Yellow Dwarf Virus Infection on Photosynthesis and Chlorophyll Fluorescence Parameters of Oat

WANG Jun, ZHAO Gui-qin, CHAI Ji-kuan, WANG Miao-miao, JIA Xue-jie, Guo Zhi-fei, SUN Lei-lei, Nie Xiu-mei

College of Pratacultural Science, Gansu Agricultural University;Key Laboratory of Grassland Ecology System, Ministry of Education, Sino-U. S. Centers for Grazing Land Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2020-02-12 Revised:2020-04-01 Online:2020-08-15 Published:2020-07-28

大麦黄矮病毒侵染对燕麦光合及叶绿素荧光参数的影响

王军, 赵桂琴, 柴继宽, 王苗苗, 贾雪洁, 郭志飞, 孙雷雷, 聂秀美

甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

通讯作者: 赵桂琴
作者简介:王军(1993-),男,甘肃庆阳人,硕士研究生,主要从事草种质资源及育种研究,E-mail:1396708336@qq.com
基金资助:
甘肃省科技计划（19ZD2NA002）和国家燕麦荞麦产业体系（CARS-7-C）资助

Abstract

Abstract: In order to investigate the photosynthetic physiological response of oat to aphid and barley yellow dwarf virus (BYDV),in this study,the changes in photosynthetic and chlorophyll fluorescence parameters in the oat leaves after inoculation with aphids and barley yellow dwarf virus at different times (0,7,14,21,28,and 35 d) were determined. The results showed that with the prolongation of the damage time,the contents of chlorophyll a,chlorophyll b and total chlorophyll decreased significantly (P<0.05). The content of Car increased significantly (P<0.05). Chl a/Chl b increased first and then decreased slightly,after 21 days of vaccination with BYDV,Chl a/Chl b increased to the maximum value,which was 57.37% higher than CK (P<0.05). Net photosynthetic rate(P_n),water use efficiency(WUE) in BYDV infected leaves decreased,intercellular CO₂ concentration(C_i),stomatal conductance(G_s),and transpiration rate (T_r) increased;meanwhile,Fluorescence (F_m),potential photochemical efficiency (F_V/F₀),maximum photochemical efficiency (F_V/F_m),photochemical quenching coefficient(qP),PS Ⅱ photochemical quantum yield(Yield),and apparent electron transport rate(ETR) showed varying degrees of reduction;non-photochemical quenching coefficient(qN) increased. This study shows that BYDV infection seriously damages the photosynthesis ability of oat leaves,and its photosynthetic parameters have changed significantly,which provides a certain reference for exploring the interaction mechanism between BYDV and oat.

Key words: Oat, BYDV, Aphid, Photosynthetic pigment, Photosynthetic gas exchange parameters, Chlorophyll fluorescence parameters

摘要： 为探究燕麦在蚜虫和大麦黄矮病毒（Barley Yellow Dwarf Virus，BYDV）为害后的光合生理响应，本试验测定了燕麦接种蚜虫和BYDV后不同时间（0，7，14，21，28和35 d）的叶片光合和叶绿素荧光参数。结果表明，随着为害时间的延长，叶片叶绿素a（Chl a）、叶绿素b（Chl b）以及总叶绿素（T-Ch1）含量显著降低（P<0.05），类胡萝卜素（Car）含量显著增加（P<0.05），Chl a/Chl b先上升后稍有下降，接种BYDV 21 d后，Chl a/Chl b均增加至最大值，比CK处理高57.37%（P<0.05）；叶片净光合速率和水分利用效率下降，胞间CO₂浓度、气孔导度和蒸腾速率上升；同时最大荧光、潜在光化学效率、最大光化学效率、光化学淬灭系数、PS Ⅱ光化学量子产量和表观光合电子传递速率均呈现下降的趋势，非光化学猝灭系数上升。本研究表明BYDV侵染严重损伤了燕麦叶片的光合作用能力，其光合参数发生了显著变化，为探讨BYDV与燕麦的互作机理提供了一定的参考依据。

关键词: 燕麦, BYDV, 蚜虫, 光合色素, 光合气体交换参数, 叶绿素荧光参数

CLC Number:

S512.6

WANG Jun, ZHAO Gui-qin, CHAI Ji-kuan, WANG Miao-miao, JIA Xue-jie, Guo Zhi-fei, SUN Lei-lei, Nie Xiu-mei. Effect of Barley Yellow Dwarf Virus Infection on Photosynthesis and Chlorophyll Fluorescence Parameters of Oat[J]. Acta Agrestia Sinica, 2020, 28(4): 923-931.

王军, 赵桂琴, 柴继宽, 王苗苗, 贾雪洁, 郭志飞, 孙雷雷, 聂秀美. 大麦黄矮病毒侵染对燕麦光合及叶绿素荧光参数的影响[J]. 草地学报, 2020, 28(4): 923-931.

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References

[1] 赖帅彬,潘新雅,简春霞,等. 转苜蓿MsOr基因烟草光合-光响应和光合-CO₂响应曲线特征研究[J]. 草地学报,2020,28(01):20-30
[2] 彭晏辉,雷娟利,黄黎锋,等. 马铃薯Y病毒侵染对叶绿体超微结构、光合和荧光参数的影响[J]. 植物病理学报,2004,34(01):32-36
[3] 张贵合,郭华春. PVY和PVS病毒复合侵染对马铃薯光合及营养品质的影响[J]. 西北植物学报,2017,37(08):1569-1576
[4] 徐品三,贾娟,孙冰轮,等. 百合无症病毒对百合光合生理、酶活性及叶绿体超微结构的影响[J]. 植物病理学报,2014,44(04):387-392
[5] 沈喜,李红玉. 病毒侵染后植物叶绿体光合作用变化的分子机制[J]. 植物病理学报,2003,33(04):289-291
[6] 付东亚,洪健,陈集双,等. 芜菁花叶病毒对寄主植物叶片PSⅡ功能的影响[J]. 植物病理学报,2004,34(02):184-186
[7] 任长忠,胡跃高. 中国燕麦学[M]. 北京:中国农业出版社,2013:1-6
[8] 雷占兰,周华坤,刘泽华,等. 密度氮肥交互处理下高寒地区燕麦的生长特性与生殖分配[J]. 草业科学,2014,31(06):1110-1119
[9] 刘振恒,武高林,仁青草,等. 发展以燕麦为支柱产业的可持续高寒草地畜牧业[J]. 草业科学,2007,24(09):67-69
[10] 王丽,霍治国,张蕾,等. 气候变化对中国农作物病害发生的影响[J]. 生态学杂志,2012,31(07):1673-1684
[11] Liu X F,Hu X S,Keller M A,et al. Tripartite Interactions of Barley Yellow Dwarf Virus,Sitobion avenae and Wheat Varieties[J]. PLoS ONE.2014,9(9):e106639
[12] 韩志勇,赵章杏,朱睦元. 大麦黄矮病毒分子生物学的研究进展[J]. 中国病毒学,1998(04):13-20
[13] 王军,赵桂琴,柴继宽,等. BYDV侵染对燕麦抗氧化防御酶、苯丙氨酸解氨酶及多酚类氧化酶的影响[J].草地学报,2020,28(01):56-63
[14] 李春杰,陈泰祥,赵桂琴,等. 燕麦病害研究进展[J]. 草业学报,2017,26(12):203-222
[15] 郭成,周天旺,郭满库,等. 291份燕麦种质资源对红叶病抗性的鉴定[J]. 草原与草坪,2019,39(02):60-65
[16] 陈福明,陈顺伟. 混合液法测定叶绿素含量的研究[J]. 林业科技通讯,1984(02):4-8
[17] Sperdouli I,Moustakas M. A better energy allocation of absorbed light in photosystem Ⅱ and less photooxidative damage contribute to acclimation of Arabidopsis thaliana young leaves to water deficit[J]. Journal of Plant Physiology,2014,171(8):587-593
[18] 李朝霞,赵世杰,孟庆伟,等. 不同粒叶比小麦品种非叶片光合器官光合特性的研究[J]. 作物学报,2004,30(05):419-426
[19] Ladjal M，Epron D，Ducrey M. Effects of drought preconditioning on thermotolerance of photosystem Ⅱ and susceptibility of photosynthesis to heat stress in cedar seedlings[J]. Tree Physiology,2000,20(18):1235-1241
[20] 陆璃,吕金印,巩擎柱,等. 空间环境对小麦叶绿素荧光及光合特性的影响[J]. 核农学报,2006,20(06):464-468
[21] 李晓,冯伟,曾晓春. 叶绿素荧光分析技术及应用进展[J]. 西北植物学报,2006,26(10):2186-2196.
[22] 于海业,张雨晴,刘爽,等. 植物叶绿素荧光光谱的研究进展[J]. 北方园艺,2017,41(24):194-198
[23] 李燕宏,洪健,谢礼,等. 蚕豆萎蔫病毒2号分离物侵染对蚕豆叶片光合活性和叶绿体超微结构的影响[J].植物生理与分子生物学学报,2006,32(04):490-496
[24] 耿东梅,单立山,李毅,等. 土壤水分胁迫对红砂幼苗叶绿素荧光和抗氧化酶活性的影响[J]. 植物学报,2014,49(03):282-291
[25] Willekens H,Vancamp W,Lnze D,et al. Ozone,sulfur dioxide,and ozone ultraviolet-B have similar effect on mRNA accumulation of antioxidant genes inNicotiana plumbaginifoliaL[J]. Plant Physiol,1994,10(6):1007-1014
[26] 冯岩,侯喜林,马景蕃,等. 芜菁花叶病毒侵染对不结球白菜光合及荧光特性的影响[J]. 江苏农业学报,2010,26(03):508-511
[27] 郭兴启,温孚江,朱汉城. 烟草感染马铃薯Y病毒(PVY)后光合作用的变化规律[J]. 浙江大学学报(农业与生命科学版),2000,26(01):77-80
[28] 王春梅,施定基,朱水芳,等. 黄瓜花叶病毒对烟草叶片和叶绿体光合活性的影响[J]. 植物学报,2000,42(04):388-392
[29] 韩清芳,贾志宽,王俊鹏,等. 黄土高原地区紫花苜蓿不同叶位光合日变化特征研究[J]. 草地学报,2009,17(05):558-563
[30] 于力,郭世荣,朱为民,等. 番茄黄化曲叶病毒对番茄叶片光合特性和叶绿体超微结构的影响[J]. 西北植物学报,2011,31(07):1355-1359
[31] 倪国仕,章新军,毕庆文,等. 受烟草花叶病毒侵染程度不同的烤烟叶片光合特性的变化[J]. 中国烟草科学,2010,31(05):58-61
[32] 寇江涛,师尚礼,胡桂馨,等. 紫花苜蓿对牛角花齿蓟马为害的光合生理响应[J]. 生态学报,2014,34(20):5782-5792
[33] Farquhar,G D,Sharkey,T D. Stomatal Conductance and Photosynthesis[J]. Annu.rev.plant Physiol,1982,33(1):317-345
[34] Wilhelmova N，Prochazkova D，Sindelarova M，et al. Photosynthesis in leaves ofNicotiana tabacumL. infected with tobacco mosaic virus[J]. Photosynthetica (Prague),2005,43(4):597-602
[35] 刘军,齐广平,康燕霞,等. 不同灌溉处理下紫花苜蓿光合特性、叶绿素荧光参数及生物量的变化[J]. 草地学报,2019,27(06):1569-1576
[36] 于力,阎君,郭世荣,等. 番茄黄化曲叶病毒侵染对番茄叶片光合及荧光特性的影响[J]. 上海农业学报,2012,28(03):22-25

Effect of Barley Yellow Dwarf Virus Infection on Photosynthesis and Chlorophyll Fluorescence Parameters of Oat

大麦黄矮病毒侵染对燕麦光合及叶绿素荧光参数的影响

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