Response of Soil Enzyme Stoichiometry to Different Enclosure Durations in Alpine Meadow,Tibetan Plateau

doi:10.11733/j.issn.1007-0435.2021.01.005

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (1): 33-41.DOI: 10.11733/j.issn.1007-0435.2021.01.005

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Response of Soil Enzyme Stoichiometry to Different Enclosure Durations in Alpine Meadow,Tibetan Plateau

MI Qi^1,2, WANG Yi^1,2, QIN Xiao-jing³, SUN Jian², YE Chong-chong^2,4

1. Chengdu University of Technology, Chengdu, Sichuan Province 610059, China;
2. Key Laboratory of Observation and Simulation of Ecological Networks, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;
3. School of Surveying and Land Information Engineering, Henan Polytechnic University, Henan Province 454000, China;
4. School of Civil Engineering and Architecture, Southwest Petroleum University, Chengdu, Sichuan Province 610500, China

Received:2020-06-15 Revised:2020-07-26 Published:2021-01-08

青藏高原高寒草甸不同围栏年限土壤酶化学计量特征

米琦^1,2, 王毅^1,2, 秦小静³, 孙建², 叶冲冲^2,4

1. 成都理工大学, 四川成都 610059;
2. 中国科学院地理科学与资源研究所生态网络观测与模拟重点实验室, 北京 100101;
3. 河南理工大学测绘与国土信息工程学院, 河南焦作, 454000;
4. 西南石油大学土木工程与建筑学院, 四川成都, 610500

通讯作者: 孙建
作者简介:米琦(1995-),男,四川广安人,硕士研究生,主要从事高原生态学研究,E-mail:mqyx1995@163.com
基金资助:
第二次青藏高原综合科学考察研究项目“生态安全屏障功能与优化体系”项（2019QZKK0405-05）资助

Abstract

Abstract: Soil enzymes play an important role in the process of community succession and nutrient conversion. With the implementation of fencing measures,it is vital to explore the driving mechanism of soil enzyme activity in the process of grassland restoration. We conducted a manipulative grazing exclusion experiment with fences lasting 2,4,6,8,and 10 years in alpine meadow,Tibetan Plateau. We investigated soil properties,soil enzyme activities and stoichiometry,and their drivers. The results showed that β-1,4-glucosidase closely related to C cycle,N-acetyl-β-D-glucosidase and leucine aminopeptidase related to N cycle and alkaline phosphatase related to P cycle,expressed an increasing trend with increasing the duration of fences. When the duration of fence reached 8 years,the ratio of soil C to N enzyme activities decreased,and was significantly negatively and positively correlated with soil water content and pH,respectively,while the ratio of soil C to P enzyme activities increased,which was significantly negative and positive correlated with soil organic carbon. In summary,soil enzyme activities and stoichiometry remarkablely varied with the different durations of fence,and the nutrients of alpine meadow was limited by the lack of nitrogen,and the sensitive responses of soil enzyme activity to the vegetation characteristics and soil properties were observed in the duration of 6 to 8 years fences.

Key words: Alpine meadow, Enclosure, Soil enzyme activity, Enzyme stoichiometry, Environmental factor

摘要： 为探究土壤酶活性和酶计量比的变化特征及影响机制，本试验以青藏高原若尔盖高寒草甸为对象，研究了围栏2，4，6，8和10年自然恢复条件下土壤理化性质和酶活性差异性规律。结果表明：随着恢复年限的增加，与碳（carbon，C）循环密切相关的β-葡萄糖苷酶、与氮（nitrogen，N）循环密切相关的N-乙酰-β-D-葡萄糖苷酶和亮氨酸氨基肽酶以及与磷（phosphorus，P）循环密切相关的碱性磷酸酶活性均呈现增加趋势；围栏8年时，土壤C：N酶活性比特征值下降，C：P酶活性比特征值上升，前者与土壤含水量和pH值呈显著负、正相关关系（P<0.05），后者与土壤有机碳显著负相关（P<0.05）。整体而言，严重沙化的高寒草甸，在围栏封育过程中受N养分限制，在围栏6~8年时，与养分循环相关的酶活性对植被特征和土壤属性的响应敏感。

关键词: 高寒草甸, 围栏封育, 土壤酶活性, 酶化学计量比, 环境因子

CLC Number:

S154.1

MI Qi, WANG Yi, QIN Xiao-jing, SUN Jian, YE Chong-chong. Response of Soil Enzyme Stoichiometry to Different Enclosure Durations in Alpine Meadow,Tibetan Plateau[J]. Acta Agrestia Sinica, 2021, 29(1): 33-41.

米琦, 王毅, 秦小静, 孙建, 叶冲冲. 青藏高原高寒草甸不同围栏年限土壤酶化学计量特征[J]. 草地学报, 2021, 29(1): 33-41.

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Response of Soil Enzyme Stoichiometry to Different Enclosure Durations in Alpine Meadow,Tibetan Plateau

青藏高原高寒草甸不同围栏年限土壤酶化学计量特征

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