Study on the Effect of the Plant Growth-Promoting Rhizobacteria Bio-fertilizer Instead of Chemical Fertilizer in Alpine Meadow

doi:10.11733/j.issn.1007-0435.2021.07.006

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (7): 1423-1429.DOI: 10.11733/j.issn.1007-0435.2021.07.006

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Study on the Effect of the Plant Growth-Promoting Rhizobacteria Bio-fertilizer Instead of Chemical Fertilizer in Alpine Meadow

ZHANG Wan-tong, LI Chao-qun, YU Lu, SHAO Xin-qing

College of Grassland Science and Technology China Agriculture University, Beijing 100193, China

Received:2021-03-08 Revised:2021-04-01 Online:2021-07-15 Published:2021-07-31

植物根际促生菌菌肥在高寒草甸替代化肥效应研究

张万通, 李超群, 于露, 邵新庆

中国农业大学草业科学与技术学院, 北京 100193

通讯作者: 邵新庆,E-mail:shaoxinqing@163.com
作者简介:张万通(1999-),男,汉族,山东菏泽人,硕士研究生,主要从事退化草地恢复研究,E-mail:wt20200909@163.com
基金资助:
国家自然科学基金项目（31971746）；退化高寒草原恢复及生态功能提升技术与示范（2016YFC0501902）资助

Abstract

Abstract: In order to explore the degradation of alpine meadow ecosystem in Qinghai-Tibet Plateau,in terms of fertilization improving the condition of grassland degradation,alpine meadow in Qinghai Province was selected as research object in this study,control without fertilization (CK),applied nitrogen fertilizer 250 kg·hm^-2(N),phosphorus fertilizer 400 kg·hm^-2 (P),nitrogen and phosphorus fertilizer 300 kg·hm^-2(NP),organic fertilizer 2 000 kg·hm^-2(O),PGPR bio-fertilizer 7.5 kg·hm^-2(J1),15 kg·hm^-2 (J2),22.5 kg·hm^-2(J3),J1+70% nitrogen fertilizer (J1+N),J1+70% phosphate fertilizer (J1+P) were conducted. The improvement effects of different fertilization treatments on alpine meadow the applicability of PGPR bio-fertilizer in alpine meadow and to what extent the bio-fertilizer can replace chemical fertilizers were analyzed. The results showed that, NP significantly increased the aboveground biomass (P<0.05). J1+N treatment could increase the aboveground biomass,alleviate the decline of species richness caused by N fertilizer application,as well as increase soil moisture and total nitrogen content (P<0.05) without causing salinization. In summary,J1+N treatment can partially replace chemical fertilizer and reduce the harm of inorganic fertilizer to ecological environment,so it is recommended to be applied in alpine meadow.

Key words: Fertilization, Alpine meadow, Plant growth-promoting rhizobacteria bio-fertilizer

摘要： 为探究青藏高原高寒草甸退化问题，从施肥可以改良草地退化的角度出发，本研究以青海省高寒草甸为研究对象，通过设置不同施肥处理，即对照不施肥（CK）、施加氮肥250 kg·hm^-2（N）、磷肥400 kg·hm^-2（P）、氮磷肥300 kg·hm^-2（NP）、有机肥2 000 kg·hm^-2（O）、植物根际促生菌（Plant growth-promoting rhizobacteria，PGPR）菌肥7.5 kg·hm^-2（J1），15 kg·hm^-2（J2），22.5 kg·hm^-2（J3）、PGPR菌肥+70%氮肥（J1+N）、PGPR菌肥+70%磷肥（J1+P），对不同施肥处理高寒草甸的改良效果、PGPR菌肥的适用性以及菌肥替代化肥的效果进行分析。结果表明：NP显著提高地上生物量（P<0.05），J1+N处理不仅增加了地上生物量，还可缓解施用N肥造成的物种丰富度下降，同时增加土壤水分和全氮含量（P<0.05），又不会引起盐渍化。综上所述，J1+N处理能够部分替代化肥，减少无机化肥对生态环境的危害，推荐在高寒草甸施用。

关键词: 施肥, 高寒草甸, 植物根际促生菌菌肥

CLC Number:

S144

ZHANG Wan-tong, LI Chao-qun, YU Lu, SHAO Xin-qing. Study on the Effect of the Plant Growth-Promoting Rhizobacteria Bio-fertilizer Instead of Chemical Fertilizer in Alpine Meadow[J]. Acta Agrestia Sinica, 2021, 29(7): 1423-1429.

张万通, 李超群, 于露, 邵新庆. 植物根际促生菌菌肥在高寒草甸替代化肥效应研究[J]. 草地学报, 2021, 29(7): 1423-1429.

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References

[1] 王玲. N、P添加对退化高寒草地植被与土壤的影响[D]. 青海:青海大学,2020:8-9
[2] Doran P T,Zimmerman M k. Examining the scientific consensus on climate change[J]. Eos.Transactions American Geophysical Union,2009(3):31-32
[3] 王玲,施建军,董全民,等. 氮、磷添加对高寒草原群落多样性和生物量的影响[J]. 草地学报,2019,27(6):1633-1642
[4] 张璐璐,李艳,王孝安,等. 刈割与施肥对高寒草甸土壤和植物N、P化学计量学特征的影响[J]. 西北植物学报,2017,37(11):2256-2264
[5] 高中超,迟凤琴,赵秋. 施肥对退化草原植物群落产量及土壤理化性质的影响[J]. 草原与草坪,2007(2):60-62
[6] 郭雨晴,赵世超,包玉英,等. 3种荒漠珍稀植物根际促生菌的筛选、鉴定及对高粱幼苗生长的影响[J]. 草地学报,2020,28(4):1121-112
[7] 白玉婷,卫智军,刘文亭,等. 草地施肥研究及存在问题分析[J]. 草原与草业,2016,28(2):7-12
[8] 荣良燕,柴强,姚拓,等. 复合微生物接种剂替代部分化肥对豌豆间作玉米的促生效应[J]. 草业学报,2015,24(2):22-30
[9] 何志刚,王秀娟,董环,等. PGPR菌肥对马铃薯产量与肥料利用率影响的初步研究[J]. 中国土壤与肥料,2013(2):100-103
[10] 孙韵雅,陈佳,王悦,等. 根际促生菌促生机理及其增强植物抗逆性研究进展[J]. 草地学报,2020,28(5):1203-1215
[11] Vessey J K. Plant growth promoting rhizobacteria as biofertilizers[J]. Plant and Soil,2003,255(2):571-586.
[12] Krey T,Caus M,Baum C,et al. Interactive effects of plant growth-promoting rhizobacteria and organic fertilization on P nutrition of Zea mays L. and Brassica napus L.[J]. Journal of Plant Nutrition and Soil Science,2011,174(4):602-613
[13] 赵晨阳,戴峰,刘述颖,等. 植物根际促生菌的研究进展[J]. 安徽农业科学,2019,47(16):12-13,24
[14] 孙运杰,马海林,刘方春,等. 生物肥对蓝莓根际土壤微生物学特性及土壤肥力的影响[J]. 水土保持学报,2015,29(3):167-171,177
[15] 徐畅,刘景辉,杨彦明,等. 菌肥与氮磷肥配施对覆膜马铃薯抗旱生理指标及产量的影响[J]. 作物杂志,2017(1):94-99
[16] 姚静,陈金强,辛晓平,等. 复合微生物肥料对羊草草原植物群落物种多样性和生物量的影响[J]. 草业学报,2017,26(10):108-117
[17] 刘博文. PGPR固体菌肥的研发及对紫花苜蓿生长和品质的影响[D]. 哈尔滨:哈尔滨师范大学,2017:11-12
[18] 张堃,姚拓,张德罡,等.高寒地区联合固氮菌肥对青稞的促生效应研究[J]. 植物营养与肥料学报,2010,16(03):708-713
[19] 马涛. 青藏高原高寒草甸植物群落结构和功能对施肥和刈割干扰的响应研究[D]. 兰州:兰州大学,2007:18-19
[20] 鲍士旦. 土壤农化分析. 第3版[M]. 北京:中国农业出版社,2000,10-17
[21] 杨中领,苏芳龙,苗原,等. 施肥和放牧对青藏高原高寒草甸物种丰富度的影响[J]. 植物生态学报,2014,38(10):1074-1081
[22] Kirchner T. The effects of resource enrichment on the diversity of plants and arthropods in a shortgrass prairie[J]. Ecology,1977,58(6):1334-1344
[23] 张春花. 放牧方式和施肥梯度对高寒草甸群落生产力和物种丰富度的影响[J]. 草业科学,2014,31(12):2293-2300
[24] Elly P H B,Jacobs F H H. Production,Nutrient Availability,and Elemental Balances of Two Meadows Affected by Different Fertilization and Water Table Regimes in the Netherlands[J]. Plant ecology,2001,155(1):61-73
[25] 贾志锋,马祥,雷生春,等. 施肥对贵南县轻度退化草甸植被特征的影响[J]. 草地学报,2019,27(4):987-996
[26] 李琦,冯影,姚拓,等. 不同剂型促生菌肥与化肥配施对紫花苜蓿生产性能及营养品质的影响[J]. 草原与草坪,2020,40(1):74-79
[27] 郑华平,陈子萱,王生荣,等. 施肥对玛曲高寒沙化草地植物多样性和生产力的影响[J]. 草业学报,2007(5):34-39
[28] Ren Z,Li Q,Chu C, et al. Effects of resource additions on species richness and ANPP in an alpine meadow community[J]. Journal of Plant Ecology,2010,3(1):25-31
[29] 张杰琦,李奇,任正炜,等. 氮素添加对青藏高原高寒草甸植物群落物种丰富度及其与地上生产力关系的影响[J].植物生态学报,2010,34(10):1125-1131
[30] 陈奇乐. 施肥对坝上草地植被与土壤理化性质的影响[D]. 保定:河北农业大学,2020:45
[31] 温超,单玉梅,高丽娟. 施肥对科尔沁羊草割草场植物群落多样性和生产力的影响[J]. 江苏农业科学,2017,45(16):250-254
[32] Paradelo R,Barral M T. Effect of moisture and disaggregation on the microbial activity of soil[J]. Soil and Tillage Research,2009,104(2):317-319
[33] 张丽芳. 土壤酸碱性对植物生长影响的研究进展[J]. 贵州农业科学,2020,48(8):40-43
[34] 段敏杰,干珠扎布,郭佳. 施肥对藏北高寒草地植物多样性及生产力的影响[J]. 西北农业学报,2016,25(11):1696-1703
[35] 魏鑫. 不同施肥处理对甘蔗生长和土壤养分及微生物的影响[D]. 福建:福建农林大学,2020:39-40
[36] 李恩宇,何贵永. 青藏高原高寒草甸不同季节土壤理化性质及酶活性对施肥处理的响应[J]. 广西植物,2014,34(4):467-472
[37] 王玲,施建军,史慧兰,等. 氮磷添加对环青海湖高寒草原牧草营养成分和土壤养分的影响[J]. 草业科学,2019,36(12):3065-3075
[38] Elwan M W M,Abd El-Azeem S A M. Effects of plant growth promoting rhizobacteria on summer squash growth,yield,nutrients uptake and availability under nitrogen and phosphorus fertilization levels[J]. Arab Universities Journal of Agricultural Sciences,2015,23(2):n497-n511,r512-r513
[39] 张福锁. 土壤与植物营养研究新动态[M]. 北京:北京农业大学出版社,1992,50-55
[40] 陈桂芬,刘忠,黄雁飞,等. 不同施肥处理对连作蔗田土壤微生物量、土壤酶活性及相关养分的影响[J]. 南方农业学报,2015,46(12):2123-2128
[41] 张秀,赵永存,谢恩泽. 土壤有机碳时空变化研究进展与展望[J]. 农业环境科学学报,2020,39(4):673-679
[42] 杨潇. 长期施肥对土壤无机碳变化的影响及机理研究[D]. 北京:中国农业科学院,2020:9-10
[43] Warembourg F R,Estelrich H D. Plant phenology and soil fertility effects on below-ground carbon allocation for an annual (Bromus madritensis) and a perennial (Bromus erectus) grass species[J]. Soil biology & biochemistry,2001,33(10):1291-1303

Study on the Effect of the Plant Growth-Promoting Rhizobacteria Bio-fertilizer Instead of Chemical Fertilizer in Alpine Meadow

植物根际促生菌菌肥在高寒草甸替代化肥效应研究

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