Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (7): 1462-1468.DOI: 10.11733/j.issn.1007-0435.2021.07.011

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Effects of Low Phosphorus Stress on the Growth and Acid Phosphatase Activity of Macrotyloma uniflorum (Lam.) Verdc. Yazhou

HUANG Jie1,2, ZHANG Lang-zhi2, XING Yu-fen1, ZOU Xiao-yan2, LIU Guo-dao1, YU Dao-geng1, CHEN Zhi-jian1,2   

  1. 1. Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan Province 571101, China;
    2. College of Tropical Crops, Hainan University, Haikou, Hainan Province 570228, China
  • Received:2021-02-23 Revised:2021-03-25 Online:2021-07-15 Published:2021-07-31

低磷胁迫对崖州硬皮豆生长及酸性磷酸酶活性的影响

黄杰1,2, 张郎织2, 邢玉芬1, 邹晓燕2, 刘国道1, 虞道耿1, 陈志坚1,2   

  1. 1. 中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室, 海南 海口 571101;
    2. 海南大学热带作物学院, 海南 海口 570228
  • 通讯作者: 陈志坚,E-mail:jchen@scau.edu.cn;虞道耿,E-mail:geng0209@126.com
  • 作者简介:黄杰(1993-),男,河南信阳人,硕士研究生,主要从事热带牧草植物营养胁迫研究,E-mail:260633452@qq.com
  • 基金资助:
    财政部和农业农村部:国家现代农业产业技术体系;滇桂黔石漠化地区特色作物产业发展关键技术集成示范项目(SMH2019-2021);海南省农业农村厅物种资源保护项目资助

Abstract: Low phosphorus (P) stress is one of the constraints on crop growth and yield. ‘Macrotyloma uniflorum (Lam.) Verdc. Yazhou’ is a high-quality forage for livestock,but its response mechanisms on low P availability are largely unknown. In this study,in order to investigate the effects of low P stress on the growth and physiological characteristics of M.uniflorum,plant growth,P concentration and acid phosphatase (ACP) activity of M.uniflorum under different P treatments (5 μM,100 μM and 250 μM KH2PO4) were analyzed in hydroponic conditions. Results showed that plant height,number of trifoliate leaf,leaf area,total root length,root surface area,root volume and, plant dry weight of M. uniflorum were significantly inhibited by 5 μM P treatment compared to 100 μM and 250 μM P treatments. In addition,5 μM P treatment significantly reduced P content,soluble P content and cell wall P content. However,increasing in the total ACP and cell wall ACP activities in leaves and roots was observed under 5 μM P condition compared to 100 μM and 250 μM P treatments. The total ACP activities in leaves and roots of M. uniflorum under 5 μM P treatment was 1.5~1.6 times higher than those under 250 μM P treatment,respectively. Furthermore,cell wall ACP activity in leaves and roots was 1.2~1.3 times higher than those under 250 μM P treatment,respectively. The results suggest that M. uniflorum could induce more acid phosphatase in response to P deficiency,thereby increasing P utilization.

Key words: Macrotyloma uniflorum, Low P stress, Acid phosphatase, P content

摘要: 本试验为探究硬皮豆(Macrotyloma uniflorum)对低磷胁迫的响应机制,以‘崖州硬皮豆’为材料,采用水培方法,分析了不同磷浓度(5 μM,100 μM和250 μM KH2PO4)处理对硬皮豆生长和生理特性的影响。结果表明:与100 μM和250 μM磷处理相比,5 μM低磷处理抑制了硬皮豆株高、复叶数、叶面积、总根长,根表面积和根体积以及植株干重。同时,5 μM磷处理显著降低了地上部和根部磷含量、降低了叶片和根系可溶性磷含量和细胞壁磷含量;与100 μM和250 μM磷处理相比,5 μM磷处理增加了叶片和根系总酸性磷酸酶(Acid phosphatase,ACP)活性和细胞壁ACP活性;5 μM磷处理下叶片和根系总ACP活性是250 μM磷条件下的1.5~1.6倍,而叶片和根系细胞壁ACP活性是250 μM磷条件下的1.2~1.3倍。因此,提高酸性磷酸酶活性可能是硬皮豆响应低磷胁迫的重要机制。

关键词: 硬皮豆, 低磷胁迫, 酸性磷酸酶, 磷含量

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