Effect of Different Dying Methods on the Mycorrhizal Infection Rate of Two Gramineous Forages

doi:10.11733/j.issn.1007-0435.2021.12.025

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (12): 2838-2844.DOI: 10.11733/j.issn.1007-0435.2021.12.025

Effect of Different Dying Methods on the Mycorrhizal Infection Rate of Two Gramineous Forages

ZHANG Hai-juan¹, LU Guang-xin¹, FAN Yue-jun^2,4, ZHOU Hua-kun², ZHOU Xue-li³, DOU Sheng-yun³, YAN Hui-lin¹, MA Kun¹, ZHAO Yang-an¹

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China;
2. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai Provincial Key Laboratory of Restoration Ecology of Cold Area, Xining, Qinghai Province 810008, China;
3. Qinghai Province Grassland Improvement Experimental Station, Gonghe, Qinghai Province 813000, China;
4. Qinghai Agriculture and Animal Husbandry Science and Techology Vocational College, Huangyuan, Qianghai Province 812100, China

Received:2021-06-01 Revised:2021-07-21 Online:2021-12-15 Published:2022-01-06
Contact: 芦光新,E-mail:lugx74@qq.com;范月君,E-mail:fanyuejun_79@163.com

不同染色方法对两种禾本科牧草菌根侵染率的影响

张海娟¹, 芦光新¹, 范月君^2,4, 周华坤², 周学丽³, 窦声云³, 颜珲璘¹, 马坤¹, 赵阳安¹

1. 青海大学农牧学院, 青海西宁 810016;
2. 中国科学院西北高原生物研究所, 青海省寒区恢复生态学重点实验室, 青海西宁 810008;
3. 青海省草原改良试验站, 青海共和 813000;
4. 青海农牧科技职业学院, 青海湟源 812100

作者简介:张海娟(1990-)，女，青海湟中人，博士研究生，主要从事草地生态与环境保护研究，E-mail：1205590222@qq.com
基金资助:
青海省科技厅基础研究面上计划项目（2021-ZJ-915）；青海省寒区恢复生态学重点实验室开放课题（2020-3）资助

Abstract

Abstract: To explore the symbiosis between arbuscular mycorrhizal (AM) fungi and gramineous forages,and promote the application of plant microorganism ecological restoration technology in the restoration of degraded alpine grassland,the infection of Glomus intraradices(GI) and Glomus mosseae (GM) on Elymus sibiricus ‘Chuancao No.2’ and Elymus nutans ‘Aba’ were investigated with pot experiment,and the influence of acid fuchsin,tryptan blue and ink vinegar staining on mycorrhizal infection rate was compared at the same time. The results showed that Elymus sibiricus ‘Chuancao No.2’ and Elymus nutans ‘Aba’ can be recognized and infested by Glomus intraradices (GI) and Glomus mosseae (GM). And the mycorrhizal infection rate and spore density of Glomus mosseae (GM) were higher than that of Glomus intraradices (GI) (P<0.05). The effect of three staining methods on mycorrhizal infection rate was different,behaved as trypan blue > acid fuchsin > ink vinegar,the difference between trypan blue and acid fuchsin was not significant,and was significant with ink vinegar (P<0.05). Thus,to ensure the observation effect of mycorrhizal,trypan blue was suggested to be preferred in detecting the mycorrhizal infection rate of gramineous forages.

Key words: Arbuscular mycorrhizal fungi, Gramineous forages, Dyeing, Mycorrhizal infection rate

摘要： 为探讨丛枝菌根（Arbuscular mycorrhizal，AM）真菌与禾本科牧草（Gramineous forages）共生情况，推进植物-微生物联合生态修复技术在退化高寒草地恢复中的应用，本研究采用盆栽试验的方法，研究了根内球囊霉（Glomus intraradices，GI）和摩西球囊霉（Glomus mosseae，GM）对‘川草2号’老芒麦（Elymus sibiricus ‘Chuancao No.2’）和‘阿坝’垂穗披碱草（Elymus nutans ‘Aba’）的侵染情况，比较了酸性品红、台盼蓝和墨水醋染色法对菌根侵染率的影响。结果表明：根内球囊霉和摩西球囊霉均能与‘川草2号’老芒麦和‘阿坝’垂穗披碱草识别共生形成菌根结构，且菌根侵染率和孢子密度均为摩西球囊霉高于根内球囊霉（P<0.05）；3种染色方法对菌根侵染率的影响存在差异，总体表现为台盼蓝>酸性品红>墨水醋，台盼蓝与酸性品红间差异不显著，与墨水醋之间差异显著（P<0.05）。因此，为保证菌根观测效果，建议在测定禾本科牧草菌根侵染率时优先选用台盼蓝染色法。

关键词: 丛枝菌根真菌, 禾本科牧草, 染色, 菌根侵染率

CLC Number:

Q336

ZHANG Hai-juan, LU Guang-xin, FAN Yue-jun, ZHOU Hua-kun, ZHOU Xue-li, DOU Sheng-yun, YAN Hui-lin, MA Kun, ZHAO Yang-an. Effect of Different Dying Methods on the Mycorrhizal Infection Rate of Two Gramineous Forages[J]. Acta Agrestia Sinica, 2021, 29(12): 2838-2844.

张海娟, 芦光新, 范月君, 周华坤, 周学丽, 窦声云, 颜珲璘, 马坤, 赵阳安. 不同染色方法对两种禾本科牧草菌根侵染率的影响[J]. 草地学报, 2021, 29(12): 2838-2844.

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Effect of Different Dying Methods on the Mycorrhizal Infection Rate of Two Gramineous Forages

不同染色方法对两种禾本科牧草菌根侵染率的影响

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