Identification of the Tubby Gene Family in Oat and It’s Response to Drought Stress

doi:10.11733/j.issn.1007-0435.2025.04.003

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (4): 1029-1038.DOI: 10.11733/j.issn.1007-0435.2025.04.003

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Identification of the Tubby Gene Family in Oat and It’s Response to Drought Stress

LI Jin^1,2, YANG Yan-jiao², JIA Zhi-feng¹, ZHOU Qing-ping², CHEN Shi-yong³

1. Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai Province 810016, China;
2. College of Grassland Resources, Southwest Minzy University, Chengdu, Sichuan Province 61004l, China;
3. College of Animal and Veterinary Sciences, Southwest Minzy University, Chengdu, Sichuan Province 61004l, China

Received:2024-06-14 Revised:2024-08-06 Online:2025-04-15 Published:2025-04-28

饲用燕麦TLP基因家族鉴定及干旱胁迫下表达模式分析

李进^1,2, 杨艳娇², 贾志锋¹, 周青平², 陈仕勇³

1. 青海大学畜牧兽医科学院, 青海西宁 810016;
2. 西南民族大学草地资源学院, 四川成都 610041;
3. 西南民族大学畜牧兽医学院, 四川成都 610041

通讯作者: 陈仕勇,E-mail:chengshi8827@163.com
作者简介:李进（1997-），男，汉族，四川南充人，博士研究生，主要从事牧草种质创新研究，E-mail： lijin921@126.com
基金资助:
四川省“十四五”饲草育种攻关项目（2021YFYZ0013）；四川省农业科技成果转化项目（2023NZZJ0002）资助

Abstract

Abstract: Tubby-like proteins （TLP） are a significant group of proteins discovered across animal and plant species that participate in various biological processes. These proteins play a crucial role in plant growth， development， and the responses to abiotic stresses. This study employed bioinformatics methods to identify 32 TLP family genes from the hexaploid oat （Avena sativa） genome. We conducted an analysis of their physicochemical properties， gene structures， phylogenetic evolution， and expression patterns across different tissues under drought stress. The results demonstrated that the amino acid count of the AsTLP genes ranged from 238 to 543 amino acids. The molecular weights of these proteins were found to be between 26.99 and 60.69 kDa， and their isoelectric points ranged from 9.00 to 9.58. These genes unevenly distributed across 17 chromosomes and possessed a large number of conserved motifs. The functional elements primarily included those related to internal hormone responses and external environmental reactions. Expression analysis indicated significant differences in the expression levels of nine AsTLP members between roots and leaves of oats. Specifically， four members showed the increased expression in all tissues under drought stress， suggesting a potential significant role in the oat’s response to drought stress. These findings provided a valuable reference for further studies on the oat TLP family and their responses to drought stress.

Key words: Avena sativa, Tubby-like proteins, Bioinformatics, Drought stress, Gene expression

摘要： 类Tubby蛋白（Tubby-like proteins， TLP）是在动植物中发现的一组参与多种生物学过程的重要蛋白，在植物生长发育和非生物胁迫响应中具有重要作用。本研究通过生物信息学方法，从六倍体饲用燕麦（Avena sativa）全基因组中鉴定出32个TLP家族基因，并对其进行理化性质、基因结构、系统进化及干旱胁迫下不同组织间表达模式分析。结果表明，AsTLPs基因的氨基酸数目为238~543 aa；蛋白的分子量介于26.99~60.69 kDa之间；等电点范围在9.00~9.58之间。所有成员具有大量保守基序，分布在17条染色体上，上游作用元件主要包括内部激素和外部环境响应。表达模式分析显示9个AsTLPs成员在燕麦根系和叶片中的表达量具有显著差异，有4个成员在干旱胁迫下各组织中表达量均上升，表明可能在燕麦干旱胁迫响应中发挥重要作用。研究结果为燕麦TLP家族及其干旱胁迫响应方面的进一步研究提供参考。

关键词: 燕麦, 类Tubby蛋白, 生物信息学, 干旱胁迫, 基因表达

CLC Number:

S544.9

LI Jin, YANG Yan-jiao, JIA Zhi-feng, ZHOU Qing-ping, CHEN Shi-yong. Identification of the Tubby Gene Family in Oat and It’s Response to Drought Stress[J]. Acta Agrestia Sinica, 2025, 33(4): 1029-1038.

李进, 杨艳娇, 贾志锋, 周青平, 陈仕勇. 饲用燕麦TLP基因家族鉴定及干旱胁迫下表达模式分析[J]. 草地学报, 2025, 33(4): 1029-1038.

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Identification of the Tubby Gene Family in Oat and It’s Response to Drought Stress

饲用燕麦TLP基因家族鉴定及干旱胁迫下表达模式分析

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