Study on the Variation of Bacterial Communities in Alpine Meadow Soils along an Altitudinal Gradient and Its Influencing Factors

doi:10.11733/j.issn.1007-0435.2025.04.008

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (4): 1085-1094.DOI: 10.11733/j.issn.1007-0435.2025.04.008

Previous Articles Next Articles

Study on the Variation of Bacterial Communities in Alpine Meadow Soils along an Altitudinal Gradient and Its Influencing Factors

JI Shuang^1,3,4, WU Hao-kun², ZHANG Shao-xiong², XIE Hui-chun^2,3,4

1. College of Geographical Sciences, Qinghai Normal University, Xining, Qinghai Province 810008, China;
2. College of Life Sciences, Qinghai Normal University, Xining, Qinghai Province 810008, China;
3. Qinghai Key Laboratory of Medicinal Plant and Animal Resources on Qinghai-Tibet Plateau, College of Life Sciences, Qinghai Normal University, Xining, Qinghai Province 810008, China;
4. Qinghai Forest Ecosystem Observation and Research Station in the Southern Qilian Mountains, Huzhu, Qinghai Province 810500, China

Received:2024-04-29 Revised:2024-06-28 Online:2025-04-15 Published:2025-04-28

不同海拔高寒草甸土壤细菌多样性特征及影响因素研究

戢爽^1,3,4, 吴昊坤², 张绍雄², 谢惠春^2,3,4

1. 青海师范大学地理科学学院, 青海西宁 810008;
2. 青海师范大学生命科学学院, 青海西宁 810008;
3. 青海省青藏高原药用动植物资源重点实验室, 青海西宁 810008;
4. 青海祁连山南坡森林生态系统国家定位观测研究站, 青海互助 810500

通讯作者: 谢惠春,E-mail:yezino.1@163.com
作者简介:戢爽（1996-），女，汉族，吉林长春人，博士研究生，主要从事土壤微生物相关研究，E-mail：jishuang126@126.com
基金资助:
青海省青藏高原药用动植物资源重点实验室项目（2020-YJ-Y40）；祁连山南坡森林生态系统国家定位研究站项目（国家林业局林规[2017]212号、青林规资[2018]712号、互政[2020]127号）资助

Abstract

Abstract: In order to explore the changes of soil bacterial community diversity along altitude and its driving factors， this paper took the alpine meadow at the altitudes from 3300 to 4500 m in Guoluo Tibetan Autonomous Prefecture of Qinghai Province as the research object， and used high-throughput sequencing technology and redundancy analysis. The results indicated that the dominant bacterial community in the 0-20 cm soil layer of alpine meadows at different altitudes in the study area was primarily composed of Actinobacteria， Proteobacteria， and Acidobacteria. The diversity of soil bacteria showed varying patterns along the elevation gradient， with the Ace and Chao1 indices exhibiting fluctuating trends as altitude increases. In terms of these indices， the soil bacterial diversity was highest at an altitude of 3900 meters and lowest at 4200 meters. Furthermore， at 4200 meters， the soil bacterial community showed significant internal variability. The bacterial community structure at 4500 m above sea level was different from that at other altitudes， and the number of unique species was abundant. The composition and diversity of soil bacterial community are affected by many soil physicochemical factors. Soil available potassium content and soil pH are the most important factors affecting the composition and diversity of soil bacteria in this region. In the subsequent scientific management of alpine meadow， it is necessary to pay attention to the changes of soil pH value and soil nutrients and to avoid soil nutrient loss as much as possible. The research results can provide references for further exploring the responses of soil bacterial community to environmental changes and maintaining the stability of alpine ecosystem.

Key words: Soil bacteria, Community diversity, Alpine meadow, Altitude

摘要： 为探究高寒草甸土壤细菌群落多样性沿海拔的变化规律及驱动因素，本文以青海省果洛藏族自治州海拔3300~4500 m的高寒草甸为研究对象，利用高通量测序技术，通过冗余分析探究不同海拔高寒草甸土壤细菌群落多样性的变化规律及其影响因素。结果表明，研究区不同海拔高寒草甸0~20 cm土层土壤细菌优势群落主要由放线菌门、变形菌门和酸杆菌门组成。土壤细菌多样性对沿海拔上升的具有不同的变化规律，Ace及Chao1指数沿海拔梯度上升均呈现出波动变化。就Ace及Chao1指数而言，海拔3900 m处土壤细菌多样性最高，而海拔4200 m处最低，且在海拔4200 m处土壤细菌群落组内差异性较大；海拔4500 m处与其余海拔处相比细菌群落结构差异较大，特有的物种数目丰富。土壤细菌群落结构组成及其多样性受诸多土壤理化因素的共同影响，土壤速效钾含量、土壤pH值是影响该区域土壤细菌组成及其多样性的极重要影响因子。

关键词: 土壤细菌, 群落多样性, 高寒草甸, 海拔

CLC Number:

S158

JI Shuang, WU Hao-kun, ZHANG Shao-xiong, XIE Hui-chun. Study on the Variation of Bacterial Communities in Alpine Meadow Soils along an Altitudinal Gradient and Its Influencing Factors[J]. Acta Agrestia Sinica, 2025, 33(4): 1085-1094.

戢爽, 吴昊坤, 张绍雄, 谢惠春. 不同海拔高寒草甸土壤细菌多样性特征及影响因素研究[J]. 草地学报, 2025, 33(4): 1085-1094.

References

[1] FALKOWSKI P G，FENCHEL T，DELONG E F. The microbial engines that drive Earth’s biogeochemical cycles[J]. Science，2008，320（5879）：1034-1039
[2] DENG Q，CHENG X L，HUI D F，et al. Soil microbial community and its interaction with soil carbon and nitrogen dynamics following afforestation in Central China[J]. Science of the Total Environment，2016，541：230-237
[3] 马学兰，周连玉，孙文娟，等. 青海不同区域农田作物土壤细菌多样性及群落结构分析[J]. 微生物学报，2024，64（4）：1142-1161
[4] 尉建埔. 氮添加对帽儿山地区六树种人工林土壤微生物生物量及群落结构的影响[D]. 哈尔滨：东北林业大学，2016：31-36
[5] CHEN J H，WU Q F，LI S H，et al. Diversity and function of soil bacterial communities in response to long-term intensive management in a subtropical bamboo forest[J]. Geoderma，2019，354：113894
[6] 李益，冯秀秀，赵发珠，等. 秦岭太白山不同海拔锐齿栎林土壤微生物群落的变化特征[J]. 林业科学，2021，57（12）：22-31
[7] BARNARD R L，OSBORNE C A，FIRESTONE M K. Changing precipitation pattern alters soil microbial community response to wet-up under a Mediterranean-type climate[J]. The ISME Journal，2015，9（4）：946-957
[8] LUO Z M，LIU J X，JIA T，et al. Soil bacterial community response and nitrogen cycling variations associated with subalpine meadow degradation on the Loess Plateau，China[J]. Applied and Environmental Microbiology，2020，86（9）：e00180-20
[9] CROWTHER T W，VAN DEN HOOGEN J，WAN J，et al. The global soil community and its influence on biogeochemistry[J]. Science，2019，365（6455）：eaav0550
[10] 赵盼盼，周嘉聪，林开淼，等. 海拔梯度变化对中亚热带黄山松土壤微生物生物量和群落结构的影响[J]. 生态学报，2019，39（6）：2215-2225
[11] YUAN Y L，SI G C，WANG J，et al. Bacterial community in alpine grasslands along an altitudinal gradient on the Tibetan Plateau[J]. FEMS Microbiology Ecology，2014，87（1）：121-132
[12] WANG N，CHENG J H，LIU Y H，et al. Relative importance of altitude shifts with plant and microbial diversity to soil multifunctionality in grasslands of north-western China[J]. Plant and Soil，2024，4（2）：1-16
[13] 孙步功，龙瑞军，孔郑，等. 青海果洛黄河源区高寒草甸CO₂释放速率研究[J]. 草地学报，2007，15（5）：449-453
[14] 刘怡萱，曹鹏熙，马红梅，等. 青藏高原土壤微生物多样性及其影响因素研究进展[J]. 环境生态学，2019， 1（6）：1-7
[15] 徐公芳. 果洛州高寒草甸牧草产量与主要气象因子的灰色关联度分析[J]. 青海草业，2020，29（2）：46-49
[16] 洪卓华，张海珍. 1967—2017年青海果洛地区气候变化及其对天然牧草的影响[J]. 中国农学通报，2018，34（33）：122-128
[17] 鲍士旦. 土壤农化分析[M]. 第三版. 北京：中国农业出版社，2000：25-114
[18] LIU C S，ZHAO D F，MA W J，et al. Denitrifying sulfide removal process on high-salinity wastewaters in the presence of Halomonas sp[J]. Applied Microbiology and Biotechnology，2016，100（3）：1421-1426
[19] CHEN S F，ZHOU Y Q，CHEN Y R，et al. Fastp： an ultra-fast all-in-one FASTQ preprocessor[J]. Bioinformatics，2018，34（17）：i884-i890
[20] MAGOČ T，SALZBERG S L. FLASH： fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics，2011，27（21）：2957-2963
[21] EDGAR R C. UPARSE： highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods，2013，10（10）：996-998
[22] STACKEBRANDT E，GOEBEL B M. Taxonomic note： A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology[J]. International Journal of Systematic and Evolutionary Microbiology，1994，44（4）：846-849
[23] WANG Q，GARRITY G M，TIEDJE J M，et al. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Applied and Environmental Microbiology，2007，73（16）：5261-5267
[24] HERNÁNDEZ-CÁCERES D，STOKES A，ANGELES-ALVAREZ G，et al. Vegetation creates microenvironments that influence soil microbial activity and functional diversity along an elevation gradient[J]. Soil Biology and Biochemistry，2022，165，2（1）：108485
[25] 林惠瑛，周嘉聪，曾泉鑫，等. 武夷山黄山松林土壤细菌群落特征沿海拔梯度的分布模式[J]. 生态学杂志，2022，41（8）：1482-1492
[26] 李聪，吕晶花，陆梅，等. 滇东南亚热带土壤细菌群落对植被垂直带变化的响应[J]. 生态环境学报，2022，31（10）：1971-1983
[27] 于健，周洪敏，满孝武，等. 长白山三种针叶树木质部微生物多样性及群落结构分析[J]. 菌物学报，2023，42（9）：1906-1917
[28] 曹丽花，杨红，刘合满，等. 西藏色季拉山不同海拔梯度土壤细菌分布特征[J]. 西部林业科学，2023，52（3）：25-32
[29] 李伊凡，王俊伟，陈永豪，等. 西藏岗巴拉山土壤细菌多样性海拔分布格局及其驱动因子[J]. 生态学报，2024，44（2）：712-722
[30] 王竹，刘扬，王芳. 大通河流域土壤细菌及氮循环功能菌群沿海拔的空间分布[J]. 环境科学，2024，45（6）：3614-3626
[31] 孙华方，李希来，金立群，等. 黄河源区斑块化退化高寒草甸土壤细菌群落多样性变化[J]. 环境科学，2022，43（9）：4662-4673
[32] 曹丽花，刘合满，杨红，等. 色季拉山不同海拔土壤微生物及真菌群落组成特征[J]. 水土保持学报，2022，36（6）：371-378
[33] 刘秉儒，张秀珍，胡天华，等. 贺兰山不同海拔典型植被带土壤微生物多样性[J]. 生态学报，2013，33（22）：7211-7220
[34] 冯汉华，吴斌，伍国清，等. 喀斯特不同植被类型土壤细菌群落与功能特征[J]. 森林与环境学报，2024，44（2）：148-156
[35] 张晓馥，白雪，张起迪，等. 禁牧对内蒙古克氏针茅草原表层土壤细菌群落结构的影响[J]. 草地学报，2023，31（11）：3376-3383
[36] 薛晓娟，李英年，杜明远，等. 祁连山东段南麓不同海拔土壤有机质及全氮的分布状况[J]. 冰川冻土，2009，31（4）：642-649
[37] 贾文雄. 祁连山气候的空间差异与地理位置和地形的关系[J]. 干旱区研究，2010，27（4）：607-615
[38] 邱巡巡，曹广超，张卓，等. 高寒农田土壤有机碳和全氮密度垂直分布特征及其与海拔的关系[J]. 土壤通报，2022，53（3）：623-630
[39] LI M S，DAI G H，MU L Q. Composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in Changbai Mountains， China[J]. Frontiers in Microbiology，2022，13（1）：1065412
[40] DEQUIEDT S，THIOULOUSE J，JOLIVET C，et al. Biogeographical patterns of soil bacterial communities[J]. Environmental Microbiology Reports，2009，1（4）：251-255
[41] LAUBER C L，HAMADY M，KNIGHT R，et al. Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale[J]. Applied and Environmental Microbiology，2009，75（15）：5111-5120
[42] 王竹，刘扬，王芳，等. 黑河上游不同植被类型土壤细菌群落多样性、功能及季节动态[J]. 环境科学，2023，44（11）：6339-6353
[43] 马富龙，韩路，冯秀花，等. 封育对昆仑山北坡温性草原植物、土壤及微生物的影响[J]. 草地学报，2023，31（11）：3364-3375
[44] HU H W，ZHANG L M，DAI Y，et al. pH-dependent distribution of soil ammonia oxidizers across a large geographical scale as revealed by high-throughput pyrosequencing[J]. Journal of Soils and Sediments，2013，13（8）：1439-1449
[45] YANG Y F，GAO Y，WANG S P，et al. The microbial gene diversity along an elevation gradient of the Tibetan grassland[J]. The ISME Journal，2014，8（2）：430-440
[46] 郭倩，王博，樊勇明，等. 半灌木扩张草原土壤细菌群落特征及其影响因素[J]. 草地学报，2024，32（5）：1410-1419
[47] LIU Z F，FU B J，ZHENG X X，et al. Plant biomass， soil water content and soil N∶P ratio regulating soil microbial functional diversity in a temperate steppe： a regional scale study[J]. Soil Biology and Biochemistry，2010，42（3）：445-450
[48] 张晓黎，孙向阳，安宝晟，等. 拉萨河流域不同生态系统类型土壤微生物群落结构特征[J]. 生态学杂志，2024，43（6）：1728-1737
[49] ZHANG X F，ZHAO L， XU S JJr，et al. Soil moisture effect on bacterial and fungal community in Beilu River （Tibetan Plateau） permafrost soils with different vegetation types[J]. Journal of Applied Microbiology，2013，114（4）：1054-1065
[50] YAO F，YANG S，WANG Z R，et al. Microbial taxa distribution is associated with ecological trophic cascades along an elevation gradient[J]. Frontiers in Microbiology，2017，8：2071
[51] SILES J A，MARGESIN R. Abundance and diversity of bacterial， archaeal， and fungal communities along an altitudinal gradient in alpine forest soils： what are the driving factors？[J]. Microbial Ecology，2016，72（1）：207-220
[52] 包文，段安民，游庆龙，等. 青藏高原气候变化及其对水资源影响的研究进展[J]. 气候变化研究进展，2024，20（2）：158-169
[53] CONRADIE T A，JACOBS K. Distribution patterns of Acidobacteriota in different fynbos soils[J]. PLoS One，2021，16（3）：e0248913

Study on the Variation of Bacterial Communities in Alpine Meadow Soils along an Altitudinal Gradient and Its Influencing Factors

不同海拔高寒草甸土壤细菌多样性特征及影响因素研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HAN Meng-jie, LIU Chao, LIANG Hong-fei, MA Hai-tao, MA Hong-bin, SHEN Yan, WANG Guo-hui. The impact of Different Altitude on Soil Seed Banks in Temperate Mountainous Grassland [J]. Acta Agrestia Sinica, 2025, 33(9): 2755-2764.
[2]	ZHAO Hai-dong, LIANG Hai-hong, HU Xiao-wen, FENG Yan-hao, CHANG Sheng-hua, LI Chun-jie, HOU Fu-jiang. The Preliminary Effects of Ecological Restoration Measures in Degraded Alpine Meadows were Evaluated based on Grassland Revegetation Index [J]. Acta Agrestia Sinica, 2025, 33(9): 3057-3067.
[3]	WANG Rui, SU Shi-feng, YIN Ya-li, ZHAO Wen, WANG Yan-long, LI Shi-xiong. Effects of Grazing on Soil Phosphorus Conversion Rate in Alpine Meadow in Spring Pasture [J]. Acta Agrestia Sinica, 2025, 33(8): 2502-2511.
[4]	DUAN Jun-guang, FANG Kai, PEI Lu, CHU Jian-min, ZHANG Jin-xin, LI Xiao-xia, WANG Ying-xin. Changes of Trade-offs Between Aboveground and Belowground Plant Biomass Across Different Grazing Intensities of Alpine Meadow [J]. Acta Agrestia Sinica, 2025, 33(8): 2575-2584.
[5]	JIANG Cheng-dong, LI Guo-rong, TONG Sheng-chun, LI Jin-fang, ZHU Hai-li, LIU Ya-bin, CHEN Weng-ting, HU Xia-song. Effects of Soil Characteristics on Vegetation Diversity in Alpine Meadow Rat Mound Patchs in the Yellow River Source [J]. Acta Agrestia Sinica, 2025, 33(8): 2652-2665.
[6]	DAI Rong, LIU Su-xing, WANG Zheng-wen, ZHOU Xia-yan, BAI Ya-jun, CAO Wen-xia. Effects of Different Fertilization Modes on Vegetation and Soil of Alpine Meadow in Gannan Prefecture [J]. Acta Agrestia Sinica, 2025, 33(7): 2184-2197.
[7]	HU Ze-long, LIANG Guo-ling, LIU Wen-hui, JU Ze-liang, LI Wen. Analysis of the Relationship between Grain Color and Functional Nutrient Components in Different Oat Varieties on the Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2025, 33(6): 1843-1851.
[8]	ZHANG Yi, HU Jian, ZHOU Qing-ping, WANG Hong-yu, CHEN Jiang-mei, WANG San-xin, WANG Jia-qi, MA Wen-ting, SUN Huan. Effects of Shrub Removal Management Measures on Soil Water Conservation Function in Alpine Shrub-encroached Grassland [J]. Acta Agrestia Sinica, 2025, 33(6): 1912-1923.
[9]	ZHENG Min-na, KANG Jia-hui, GONG Rui-jie, CHEN Yan-ni, HAN Zhi-shun, LIANG Xiu-zhi. Effects of Various Alfalfa-Crop（Forage）Rotation Patterns on Bacterial Community Composition and Ecological Function Prediction [J]. Acta Agrestia Sinica, 2025, 33(5): 1387-1397.
[10]	WANG Jia-jing, HE Si-teng, LIANG Xin-ran, YANG Meng-jiao, ZHANG Ya, YANG Zhi-xin, HE Yong-mei. The Impact of Euphorbia jolkinii Boiss. Invasion on the Soil-Plant Carbon， Nitrogen， and Phosphorus Ecological Stoichiometry in Subalpine Meadows of Northwest Yunnan [J]. Acta Agrestia Sinica, 2025, 33(5): 1523-1533.
[11]	WANG Wen-bi, LIU Meng-yao, OU Ke-jie, HAO Biao-biao, GONG Jun-qiang, SU Jun-hu. Dynamics of Soil Stoichiometric Ratios and Enzyme Activities in Plateau Zokor （Eospalax baileyi） Mounds [J]. Acta Agrestia Sinica, 2025, 33(4): 1127-1136.
[12]	WEI Wei-dong, LIU Yu-hong, MA Hui. Study on Photosynthetic Physiological Traits of Plant Leaves of Different Vegetation Types in Zequ Wetland [J]. Acta Agrestia Sinica, 2025, 33(4): 1145-1155.
[13]	JIN XIN, ZHOU Xue-li, ZHENG Kai-fu, WANG Ying-cheng, WANG Jun-bang, LU Guang-xin. Effects of Alpine Meadow Degradation on Vegetation Communities in the Qilian Mountains [J]. Acta Agrestia Sinica, 2025, 33(4): 1249-1257.
[14]	WU Yan-ru, YANG Wen-quan, LIU Jia-qing, KOU Wei-liang, YU Yang-hua, LIU Qian, ZHANG Yang-can, LI Xi-lai, KOU Jian-cun. Screening,Identification,and Growth Promoting Effects Analysis of A Highly Efficient Phosphorus Solubilizing Bacterium [J]. Acta Agrestia Sinica, 2025, 33(2): 410-418.
[15]	HUO Qian-qian, SONG Yang-yang, DUAN Zhen-yu, HE Hai-xiu, CHEN Yan-bin, XI Lin-qiao. Effects of Reseeding on Soil Physicochemical Properties and Microbial Community Structure in Yili Natural Mowing Land [J]. Acta Agrestia Sinica, 2025, 33(2): 429-439.