Characteristics of Stem Strength and Lignin Synthesis Genes Expression of Oat Cultivars with Different Lodging

doi:10.11733/j.issn.1007-0435.2025.05.003

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (5): 1363-1371.DOI: 10.11733/j.issn.1007-0435.2025.05.003

Characteristics of Stem Strength and Lignin Synthesis Genes Expression of Oat Cultivars with Different Lodging

NAN Ming¹, MA Yan-ming², CHAI Ji-kuan², LIU Ya-feng², LI Xuan², ZHAO Gui-qin², XIANG Da-bing³, MIN Geng-mei¹, ZHANG Li-juan¹

1. Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu Province 730070, China;
2. College of Pratacultural Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
3. Chengdu University/Key Laboratory of Grain Processing, Ministry of Agriculture and Rural Affairs, Chengdu, Sichuan Province 610106, China

Received:2024-11-28 Revised:2024-12-31 Online:2025-05-15 Published:2025-05-20

不同倒伏性燕麦茎秆强度及其木质素合成基因表达特点

南铭¹, 马艳明², 柴继宽², 刘亚锋², 李璇², 赵桂琴², 向达兵³, 闵庚梅¹, 张丽娟¹

1. 甘肃省农业科学院作物研究所, 甘肃兰州 730070;
2. 甘肃农业大学草业学院, 甘肃兰州 730070;
3. 成都大学/农业农村部杂粮加工重点实验室, 四川成都 610106

通讯作者: 赵桂琴,E-mail:zhaogq@gsau.edu.cn
作者简介:南铭（1983-），男，汉族，甘肃通渭人，博士，副研究员，主要从事燕麦优异基因资源挖掘及种质创新研究，E-mail：cedar_mingming@163.com
基金资助:
甘肃省农业科学院博士基金（2023GAAS44）；国家现代农业产业技术体系（CARS-07-C-1）；农业农村部杂粮加工重点实验室开放课题（2023CC015）资助

Abstract

Abstract: In this study， two oat varieties with different lodging resistance were taken as materials， the regulation of lignin biosynthesis on oat stem strength was investigated by analyzing the differences of stem strength traits and lignin synthesis-related gene expression levels， which provided references for genetic improvement of oat stem strength and lodging resistance breeding.The results showed as follows： there were significant differences （P<0.05） in stem strength related traits among different lodging resistant varieties， and there was a significant positive correlation （P<0.01） between stem strength and lignin content of the basal second internode， and the lignin content of the basal second internode of lodging resistant varieties was significantly higher （P<0.05） than lodging senstive varieties. The expression levels of the relate enzyme genes of 9 lignin synthesis pathways were observed in filling stage < dough stage < milking stage， and lodging sensitive varieties < lodging resistant varieties during different growth stages. There was significant correlation between the lignin content in the basal second internode and the expression of various enzyme genes from the milking stage to dough stage. It was speculated that PAL， 4CL， C4H， CCR， C3H and COMT were the main enzyme genes involved in the lignin anabolic metabolism of oat stem.

Key words: Oat, Stem strength, Lignin synthesis, Gene expression, Lodging resistance

摘要： 本研究以2个抗倒性不同的燕麦（Avena sativa L.）品种为材料，通过分析茎秆强度相关性状及其木质素合成相关基因表达量的差异，探究木质素生物合成对燕麦茎秆强度的调控关系，为燕麦茎秆强度的遗传改良与抗倒伏育种提供参考。结果表明：茎秆强度相关性状在不同抗倒伏性品种间存在显著差异（P<0.05），基部节间茎秆强度与木质素含量极显著正相关（P<0.01），抗倒伏品种茎秆基部第2节间木质素含量显著高于易倒伏品种（P<0.05）。9个木质素合成途径相关酶基因的表达量灌浆期<蜡熟期<乳熟期，不同生育时期间均表现为易倒伏品种<抗倒伏品种。乳熟期至蜡熟期，茎秆基部第2节间木质素含量与其合成基因表达量间存在显著相关性，推测PAL，4CL，C4H，CCR，C3H，COMT是参与燕麦茎秆木质素合成代谢的主要酶基因。

关键词: 燕麦, 茎秆强度, 木质素合成, 基因表达, 倒伏性

CLC Number:

S544.9

NAN Ming, MA Yan-ming, CHAI Ji-kuan, LIU Ya-feng, LI Xuan, ZHAO Gui-qin, XIANG Da-bing, MIN Geng-mei, ZHANG Li-juan. Characteristics of Stem Strength and Lignin Synthesis Genes Expression of Oat Cultivars with Different Lodging[J]. Acta Agrestia Sinica, 2025, 33(5): 1363-1371.

南铭, 马艳明, 柴继宽, 刘亚锋, 李璇, 赵桂琴, 向达兵, 闵庚梅, 张丽娟. 不同倒伏性燕麦茎秆强度及其木质素合成基因表达特点[J]. 草地学报, 2025, 33(5): 1363-1371.

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Characteristics of Stem Strength and Lignin Synthesis Genes Expression of Oat Cultivars with Different Lodging

不同倒伏性燕麦茎秆强度及其木质素合成基因表达特点

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