Analysis of Community Composition and Diversity of Rhizosphere Soil Fungal Community and Endophytic Fungal Community in Allium senescens L.

doi:10.11733/j.issn.1007-0435.2025.06.003

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (6): 1736-1748.DOI: 10.11733/j.issn.1007-0435.2025.06.003

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Analysis of Community Composition and Diversity of Rhizosphere Soil Fungal Community and Endophytic Fungal Community in Allium senescens L.

SONG Xue¹, QI Jing-jing¹, FU Chu-han¹, XIAO Zheng-qin¹, JIA Ming-han¹, KONG Yu-dan¹, XIANG Ji-hong², QIN Li-gang¹

1. College of Animal Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, China;
2. Grassland Station of Heilongjiang Province, Harbin, Heilongjiang Province 150000, China

Received:2024-10-08 Revised:2025-03-02 Online:2025-06-15 Published:2025-06-13

山韭根际和内生真菌群落组成与多样性分析

宋雪¹, 戚晶晶¹, 付楚涵¹, 肖正琴¹, 贾茗涵¹, 孔雨丹¹, 项继红², 秦立刚¹

1. 东北农业大学动物科学技术学院, 黑龙江哈尔滨 150030;
2. 黑龙江省草原站, 黑龙江哈尔滨 150000

通讯作者: 秦立刚,E-mail:qinligang@neau.edu.cn
作者简介:宋雪（2000-），女，满族，辽宁本溪人，硕士研究生，从事草地植物资源利用研究，E-mail：songxue2023@163.com
基金资助:
国家自然科学基金（32271770）；黑龙江省自然科学基金优秀青年项目（YQ2023C013）资助

Abstract

Abstract: Allium senescens L. （A. senescens） is a common grassland plant with multiple values such as medicinal， edible， ornamental， and ecological restoration. To explore the characteristics and functional diversity of rhizosphere fungal community and endophytic fungal community of A. senescens， including rhizosphere soil， seeds， roots， bulbs， and leaves， the study was carried out using ITS amplicon sequencing technology， combining with related network analysis and function prediction methods. The results showed that the dominant phyla of rhizosphere and endophytic fungi in A. senescens were Ascomycota （56.86%） and Basidiomycota （20.47%）. The composition and diversity of fungal communities in different niches of A. senescens were different. The abundance and diversity of endophytic fungal communities were the highest in the roots of A. senescens， and the lowest in seeds. Linear discriminant analysis Effect Size （LEfSe） analysis showed that there were 64 markers of rhizosphere and endophytic fungi in A. senescens， which were mainly distributed in rhizosphere soil， roots and bulbs. The rhizosphere and endophytic fungi of A. senescens were mainly pathotroph-saprotroph-symbiotroph. In summary， the diversity of endophytic bacteria in A. senescens was rich， and the composition of fungi in different ecological niches was different， which laid a foundation for the development and functional utilization of endophytic fungi in A. senescens.

Key words: Endophytic fungal community, ITS, Ecological niches, Function prediction

摘要： 山韭（Allium senescens L.）是野生优良葱属植物，具药用、食用、观赏、生态修复等多重价值。为探明山韭根际土、种子、根、鳞茎、和叶五个生态位中真菌群落特征及多样性，采用ITS扩增子测序技术，并结合相关网络分析和功能预测方法展开研究。研究结果显示：山韭根际和内生真菌优势菌门为子囊菌门（Ascomycota，56.86%）和担子菌门（Basidiomycota，20.47%）。山韭不同生态位真菌群落组成和多样性存在差异，山韭根内生真菌群落丰度和多样性最高，种子内生真菌群落丰度和多样性最低。通过线性判别分析（Linear discriminant analysis Effect Size，LEfSe）确定山韭根际和内生真菌标志物有64个，主要分布于根际土、根和鳞茎。山韭根际和内生真菌的营养型以病理-腐生-共生营养型为主。综上，山韭内生菌多样性丰富，且不同生态位真菌组成有差异，研究为山韭内生菌菌种开发和功能利用奠定了基础。

关键词: 内生真菌群落, ITS, 生态位, 功能预测

CLC Number:

S647
S154.3

SONG Xue, QI Jing-jing, FU Chu-han, XIAO Zheng-qin, JIA Ming-han, KONG Yu-dan, XIANG Ji-hong, QIN Li-gang. Analysis of Community Composition and Diversity of Rhizosphere Soil Fungal Community and Endophytic Fungal Community in Allium senescens L.[J]. Acta Agrestia Sinica, 2025, 33(6): 1736-1748.

宋雪, 戚晶晶, 付楚涵, 肖正琴, 贾茗涵, 孔雨丹, 项继红, 秦立刚. 山韭根际和内生真菌群落组成与多样性分析[J]. 草地学报, 2025, 33(6): 1736-1748.

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Analysis of Community Composition and Diversity of Rhizosphere Soil Fungal Community and Endophytic Fungal Community in Allium senescens L.

山韭根际和内生真菌群落组成与多样性分析

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