Responses of Rhizosphere Bacteria to Grazing in Alpine Meadow of Henan County, Qinghai Province

doi:10.11733/j.issn.1007-0435.2025.10.014

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (10): 3263-3271.DOI: 10.11733/j.issn.1007-0435.2025.10.014

Responses of Rhizosphere Bacteria to Grazing in Alpine Meadow of Henan County, Qinghai Province

WANG Tian¹, CHEN Lan¹, XIE Yong-li^1,2, LU Hai-nian¹, XU Meng-ru¹, YANG Xue¹

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China;
2. State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Xining, Qinghai Province 810016, China

Received:2024-08-22 Revised:2024-12-22 Published:2025-10-17

青海省河南县高寒草甸根际土壤细菌对放牧的响应

王添¹, 陈兰¹, 谢永丽^1,2, 陆海年¹, 许梦茹¹, 杨雪¹

1. 青海大学农牧学院, 青海西宁 810016;
2. 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 青海西宁 810016

通讯作者: 谢永丽,E-mail:qhdxxyl@163.com
作者简介:谢永丽,E-mail:qhdxxyl@163.com
基金资助:
青海省科技厅应用基础研究项目（2023-ZJ-709）；国家自然科学基金（32160030）资助

Abstract

Abstract: This study investigated the response mechanisms of rhizosphere soil bacterial communities to grazing intensities in alpine meadows of the Qinghai-Tibet Plateau. Four experimental plots with no grazing （NG）， light grazing （LG）， moderate grazing （MG）， and heavy grazing （HG） intensities were established in Henan County， Qinghai Province. Combining 16S rRNA gene amplicon sequencing and soil physicochemical analysis， we systematically analyzed grazing impacts on microbial community structure and function. Results showed that bacterial α-diversity in NG was significantly higher than other treatments （P<0.05）. Characteristic phyla including Candidatus Saccharibacteria emerged in MG and HG， but dominant phyla remained stable across treatments. Soil analysis revealed organic carbon was 28.6% higher in HG than LG （P<0.05）， pH decreased and ammonium-N increased by 41.2% while nitrate-N decreased by 63.8% along grazing gradients. Spearman analysis identified significant correlations between key genera and soil parameters： Pseudonocardia and Acidibacter showed positive correlations with ammonium-N， Gp17 was negatively correlated with organic carbon， while Gp16 exhibited negative correlation with nitrate-N. The findings suggested appropriate grazing （LG-MG） maintained higher microbial diversity， but intense grazing simplified community structure. We recommend maintaining below 60% grassland utilization rate of grazing intensity to ensure ecological sustainability of alpine meadows.

Key words: Grazing intensity, Alpine meadow, Ahizosphere bacterial microbiota, Soil physicochemical properties

摘要： 本文对青藏高原高寒草甸生态系统不同放牧强度下根际土壤细菌群落响应机制展开研究。通过在青海河南县设置禁牧（NG）、轻度放牧（LG）、中度放牧（MG）和重度放牧（HG）4个梯度试验样地，运用16S rRNA基因测序技术结合土壤理化分析，系统解析放牧强度对微生物群落结构及功能的影响。结果表明：禁牧区细菌α多样性指数显著高于其他处理（P<0.05）；MG和HG处理中检测到Candidatus Saccharibacteria等特征菌门，但主要门水平组成无显著差异。土壤理化指标显示，HG处理有机碳含量较LG显著增加28.6%（P<0.05）；随放牧强度增加，土壤pH值下降，铵态氮含量上升41.2%而硝态氮含量降低63.8%。Spearman相关性分析揭示关键菌属与土壤参数存在显著关联：Pseudonocardia和Acidibacter与铵态氮呈正相关，Gp17与有机碳负相关，而Gp16则与硝态氮负相关。因此，适度放牧（LG-MG）可维持较高微生物多样性，但高强度放牧导致菌群结构简化，建议将放牧强度控制在60%草地利用率以下以保障高寒草甸生态安全。

关键词: 放牧强度, 高寒草甸, 根际细菌, 土壤理化性质

CLC Number:

S812.2

WANG Tian, CHEN Lan, XIE Yong-li, LU Hai-nian, XU Meng-ru, YANG Xue. Responses of Rhizosphere Bacteria to Grazing in Alpine Meadow of Henan County, Qinghai Province[J]. Acta Agrestia Sinica, 2025, 33(10): 3263-3271.

王添, 陈兰, 谢永丽, 陆海年, 许梦茹, 杨雪. 青海省河南县高寒草甸根际土壤细菌对放牧的响应[J]. 草地学报, 2025, 33(10): 3263-3271.

References

[1] 宋明华，刘丽萍，陈锦，等. 草地生态系统生物和功能多样性及其优化管理[J]. 生态环境学报， 2018， 27（6）: 1179-1188
[2] WANG L， LUAN L L， HOU F J， et al. Nexus of grazing management with plant and soil properties in northern China grasslands[J]. Scientific Data， 2020， 7（1）:39
[3] KARAMI P，BANDAK I.， GORGIN K M. Comparing the effects of continuous grazing and long term exclosure on floristic composition and plant diversity in rangeland ecosystems of Saral Iran[J]. International Journal of Environmental Science and Technology， 2019， 16（12）:7769
[4] 周华坤，周立，赵新全，等. 青藏高原高寒草甸生态系统稳定性研究[J]. 科学通报， 2006（1）: 63-69
[5] 赵生龙，左小安，张铜会，等.乌拉特荒漠草原群落物种多样性和生物量关系对放牧强度的响应[J].干旱区研究， 2020，37（1）:168-177
[6] 甄子雲，赵洋，姜群鸥，等.野外模拟降雨条件下高寒草甸区植被退化和人工植被恢复坡面的产流产沙过程[J].水土保持学报，2024，38（4）:153-161
[7] JIANG M， LIU J， SUN H， et al. Soil microbial diversity and composition response to degradation of the alpine meadow in the southeastern Qinghai-Tibet Plateau[J]. Environmental Science and Pollution Research， 2024 ，31（17）:26076-26088
[8] LI C， LI X， YANG Y， SHI Y， et al. Degradation reduces the diversity of nitrogen-fixing bacteria in the alpine wetland on the Qinghai-Tibet Plateau[J].Frontiers in Plant Science， 2022 ，4（13）:939762
[9] 吴佳芯，李邵宇，韩国栋.不同放牧强度下荒漠草原土壤化学计量失衡与微生物碳利用效率的联系[J].应用生态学报，2024，35（8）:2108-2118
[10] CHEN Q， SHANG Y， ZHU R， et al. Long-term enclosure at heavy grazing grassland affects soil nitrification via ammonia-oxidizing bacteria in Inner Mongolia[J]. Scientific Reports， 2022，12（1）:21464
[11] MENDES R， GARBEVA P， RAAIJMAKERS J M. The rhizosphere microbiome: significance of plant beneficial， plant pathogenic， and human pathogenic microorganisms[J]. FEMS Microbiology Reviews， 2013 ，37（5）:634-63
[12] TRIVEDI P， LEACH JE， TRINGE SG， et al. Plant-microbiome interactions: from community assembly to plant health[J]. Nature Reviews Microbiology， 2020 ，18（11）:607-621
[13] 袁鹏飞，刘文瑜，杨发荣，等.农田土壤根际微生物群落多样性特征研究[J].西北农业学报，2024，33（9）:1714-1723
[14] PAN H， LIU H， LIU Y， et al. Understanding the relationships between grazing intensity and the distribution of nitrifying communities in grassland soils[J]. Science of the Total Environment， 2018，1（634）:1157-1164
[15] SU J， JI W， SUN X， et al. Effects of different management practices on soil microbial community structure and function in alpine grassland[J]. Journal of Environmental Management， 2023， 327: 116859
[16] 张蒙蒙，徐国策，张铁钢，等.放牧强度对荒漠草原土壤微生物群落特征的影响[J].水土保持学报，2024，38（3）:306-313
[17] 邬嘉华，王立新，张景慧，等.温带典型草原土壤理化性质及微生物量对放牧强度的响应[J].草地学报，2018，26（4）:832-840
[18] BUZHDYGAN OY， TIETJEN B， RUDENKO SS， et al. Direct and indirect effects of land-use intensity on plant communities across elevation in semi-natural grasslands[J]. Plos One， 2020 ，15（11）:e0231122
[19] 萨茹拉，侯向阳，陈海军，等.放牧强度对典型草原土壤微生物特征的影响[J].中国草地学报，2013，35（5）:86-91
[20] WANG F， LI Z， FU B， et al. Short-Term Grazing Exclusion Alters Soil Bacterial Co-occurrence Patterns Rather Than Community Diversity or Composition in Temperate Grasslands[J]. Front Microbiol， 2022，29（13）:824192
[21] ZHANG X， FENG Q， CAO J， et al. Grazing practices affect soil microbial networks but not diversity and composition in alpine meadows of northeastern Qinghai-Tibetan plateau[J]. Environmental Research， 2023，15（235）:116656
[22] 蒲宁宁，孙宗玖，范燕敏，等.放牧强度对昭苏草甸草原土壤有机碳及微生物碳的影响[J].新疆农业大学学报，2013，36（1）:66-70
[23] CHEN Y， CAO T， LV M， et al. Grazing Effects of Soil Fauna on White-Rot Fungi: Biomass， Enzyme Production and Litter Decomposition Ability[J].Journal of Fungi， 2022，8（4）:348
[24] 王鑫朝，韩一林，李美，等.放牧对冷蒿根际土壤微生物量碳、氮和土壤呼吸的影响[J].浙江农林大学学报，2017，34（5）:798-807
[25] FERNáNDEZ-GUISURAGA J M， CALVO L， ANSOLA G， et al. The effect of sheep grazing abandonment on soil bacterial communities in productive mountain grasslands[J]. Science of the Total Environment， 2022，851（Pt 2）:158398
[26] 贾涛涛. 黄土高原草地土壤微生物对放牧的响应[D].杨凌:西北农林科技大学，2023:26-29
[27] BREIDENBACH A， SCHLEUSS P M， LIU S， SCHNEIDER D， et al. Microbial functional changes mark irreversible course of Tibetan grassland degradation[J]. Nature Communication. 2022 ，13（1）:2681
[28] 孟令旭.牦牛觅食行为对高寒草甸土壤细菌群落特征及其功能的影响[D].呼和浩特:内蒙古大学，2023:7-16
[29] 陈峰，张静，韩二牛等.乌拉特天然梭梭（Haloxylon ammodendron）林土壤微生物多样性及其与土壤性质的关系[J].中国沙漠，2022，42（2）:207-214
[30] 张骏达. 北京市城区绿地土壤理化性质及细菌群落结构研究[D].北京:北京林业大学，2020:6-16
[31] ZHANG X， LI X， CHEN F， et al. Selenium Nanomaterials Enhance the Nutrients and Functional Components of Fuding Dabai Tea[J]. Nanomaterials， 2024，14（8）:681
[32] XIUJIE W， WEIQI W， JING Z， et al. Dominance of Candidatus saccharibacteria in SBRs achieving partial denitrification: effects of sludge acclimating methods on microbial communities and nitrite accumulation[J]. RSC Advances， 2019，9（20）:11263-11271
[33] 俞彤，唐洪旺，王常慧，等.放牧增加草地生态系统植物氮磷浓度和回收效率[J]. 草地学报， 2025， 33（2）: 566-574

Responses of Rhizosphere Bacteria to Grazing in Alpine Meadow of Henan County, Qinghai Province

青海省河南县高寒草甸根际土壤细菌对放牧的响应

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	GUO Fang, ZHANG Wei-qing, GUAN Hai-bo, WAN Zhi-qiang, YUAN Ya-nan, TSEDEVDORJ Ser-od, ZHANG Li. Characteristics of Ground-dwelling Arthropods Communities in Typical Grasslands of Inner Mongolia under Different Grazing Intensities [J]. Acta Agrestia Sinica, 2025, 33(9): 2950-2961.
[2]	ZHAO Hai-dong, LIANG Hai-hong, HU Xiao-wen, FENG Yan-hao, CHANG Sheng-hua, LI Chun-jie, HOU Fu-jiang. The Preliminary Effects of Ecological Restoration Measures in Degraded Alpine Meadows were Evaluated based on Grassland Revegetation Index [J]. Acta Agrestia Sinica, 2025, 33(9): 3057-3067.
[3]	WANG Rui, SU Shi-feng, YIN Ya-li, ZHAO Wen, WANG Yan-long, LI Shi-xiong. Effects of Grazing on Soil Phosphorus Conversion Rate in Alpine Meadow in Spring Pasture [J]. Acta Agrestia Sinica, 2025, 33(8): 2502-2511.
[4]	QI Hai-bo, FAN Jun-mei, ZHAO Wei, WANG Ming-jiu, WU A-rong, MA Yi-ming, LI Shu-ning, SUO Yuan, WU Qian. The Agronomic Characteristics of Different Forage Soybean Varieties and their Effects on Soil Nutrients and Enzyme Activities after Incorporation as Green Manure [J]. Acta Agrestia Sinica, 2025, 33(8): 2512-2520.
[5]	ZHANG Li, ZHANG Wei-qing, GUAN Hai-bo, YUAN Ya-nan, CHUN Feng, WAN Zhi-qiang, GUO Fang. Characteristics of Soil Aggregate Nematode Community in Typical Steppe of Inner Mongolia under Different Grazing Intensities [J]. Acta Agrestia Sinica, 2025, 33(8): 2531-2540.
[6]	ZHANG Lei, ZHANG Yu-juan, SUN Shi-xian, XU Xue-bao, XING Yue, LIU Xiao-li, GAO Cui-ping, WANG Cheng-jie. Effects of Different Grazing Intensities on Soil Organic Carbon Content in Desert Steppe [J]. Acta Agrestia Sinica, 2025, 33(8): 2541-2547.
[7]	DUAN Jun-guang, FANG Kai, PEI Lu, CHU Jian-min, ZHANG Jin-xin, LI Xiao-xia, WANG Ying-xin. Changes of Trade-offs Between Aboveground and Belowground Plant Biomass Across Different Grazing Intensities of Alpine Meadow [J]. Acta Agrestia Sinica, 2025, 33(8): 2575-2584.
[8]	JIANG Cheng-dong, LI Guo-rong, TONG Sheng-chun, LI Jin-fang, ZHU Hai-li, LIU Ya-bin, CHEN Weng-ting, HU Xia-song. Effects of Soil Characteristics on Vegetation Diversity in Alpine Meadow Rat Mound Patchs in the Yellow River Source [J]. Acta Agrestia Sinica, 2025, 33(8): 2652-2665.
[9]	SUN Ran, QI Xue-zhong, KAZHUO cairang, ZHOU Hua-kun, LI Hong-lin. Temporal Effects of Artificially Assisted Restoration on Soil Physicochemical Properties and Soil Microorganisms of Grasslands in the Sanjiangyuan Area [J]. Acta Agrestia Sinica, 2025, 33(7): 2172-2183.
[10]	DAI Rong, LIU Su-xing, WANG Zheng-wen, ZHOU Xia-yan, BAI Ya-jun, CAO Wen-xia. Effects of Different Fertilization Modes on Vegetation and Soil of Alpine Meadow in Gannan Prefecture [J]. Acta Agrestia Sinica, 2025, 33(7): 2184-2197.
[11]	LI Yan-chun, LI Qiang-feng, LI Wei, PENGMAO Dang-zhi, LYU Jia. Study on Soil Microbial Community Diversity of Typical Plant Communities in the Qaidam Haloxylon Ecosystem Reserve [J]. Acta Agrestia Sinica, 2025, 33(7): 2227-2237.
[12]	ZHOU Hui-min, GAO Zhi-wei, ZHANG Xiao-jia, WU Jia-xin, WANG Wei-feng, HAN Guo-dong. Effects of Different Grazing Intensity on Soil Bacterial Community in Inner Mongolian Desert Grasslands [J]. Acta Agrestia Sinica, 2025, 33(6): 1764-1770.
[13]	ZHANG Yi, HU Jian, ZHOU Qing-ping, WANG Hong-yu, CHEN Jiang-mei, WANG San-xin, WANG Jia-qi, MA Wen-ting, SUN Huan. Effects of Shrub Removal Management Measures on Soil Water Conservation Function in Alpine Shrub-encroached Grassland [J]. Acta Agrestia Sinica, 2025, 33(6): 1912-1923.
[14]	SHI Rong-xi, WANG Quan-cheng, LIU Ning, LIU Meng-jie, WANG Jin-song, HU Jian, WANG Hui, CHEN You-jun, ZHOU Qing-ping. Long-term Drainage Reduces Soil Enzyme Activities in Alpine Peatlands on the Zoige Plateau [J]. Acta Agrestia Sinica, 2025, 33(5): 1398-1407.
[15]	WANG Jia-jing, HE Si-teng, LIANG Xin-ran, YANG Meng-jiao, ZHANG Ya, YANG Zhi-xin, HE Yong-mei. The Impact of Euphorbia jolkinii Boiss. Invasion on the Soil-Plant Carbon， Nitrogen， and Phosphorus Ecological Stoichiometry in Subalpine Meadows of Northwest Yunnan [J]. Acta Agrestia Sinica, 2025, 33(5): 1523-1533.