Effects of Additives on Fermentation Quality and Microbial Quantity of Elymus dahuricus silage

doi:10.11733/j.issn.1007-0435.2026.02.030

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (2): 679-686.DOI: 10.11733/j.issn.1007-0435.2026.02.030

Effects of Additives on Fermentation Quality and Microbial Quantity of Elymus dahuricus silage

WANG Wei, ZHAO Muqier, WANG Zhi-jun, GE Gen-tu

1. College of Grassland Science, Inner Mongolia Agricultural University Key Laboratory of Forage Cultivation and the Processing and Highly Efficient Utilization of the Ministry of Agriculture/KeyLaboratory of Grassland Resources of the Ministry of Education, Hohhot, Inner Mongolia 010011, China

Received:2025-04-02 Revised:2025-07-06 Published:2026-01-22

添加剂对披碱草青贮发酵品质及微生物数量的影响

王伟, 赵牧其尔, 王志军, 格根图

1. 内蒙古农业大学草业学院/农业农村部饲草栽培、加工与高效利用重点实验室/草地资源教育部重点实验室, 内蒙古呼和浩特 010011

通讯作者: 格根图，E-mail:gegentu@163.com
作者简介:王伟（1999-），男，汉族，安徽滁州人，硕士研究生，主要从事饲草加工与利用方向研究，E-mail:2737217721@qq.com；
基金资助:
财政部和农业农村部国家现代农业产业技术体系（CARS-34）资助

Abstract

Abstract: To investigate the effects of different additives on the silage quality and microbial population of Elymus dahuricus，Elymus dahuricus was used as the raw material and herein and set up five treatment groups： P₁ （no additive， add the same amount of distilled water）， P₂ （adding Lactobacillus plantarum，the addition amount was 1×10⁶ CFU·g^-1 FW）， P₃（adding molasses， the addition amount was 20 g·kg^-1 FW）， P₄ （adding Lactobacillus buchneri，the addition amount was 1×10⁶ CFU·g^-1FW）， and P₅ （adding cellulase，the addition amount was 50 U·g^-1 FW）. After 60 days of ensiling， the nutritional quality， fermentation characteristics， and microbial counts were determined. The results showed that different silage additives significantly affected the contents of water soluble carbohydrates （WSC）， neutral detergent fiber （NDF）， acid detergent fiber （ADF）， and crude ash in Elymus dahuricus silage（P<0.05）. The additives significantly decreased the pH value of the silage and significantly increased the lactic acid content （P<0.05）， while significantly reducing the propionic acid content （P<0.05）. Comprehensive membership function analysis indicated that quality of Elymus dahuricus silage was the best when the addition amount was 20g·kg^-1 FW molasses.

Key words: Elymus dahuricus, Additives, Silage quality, Microbial quantity

摘要： 为探究不同添加剂对披碱草（Elymus dahuricus）青贮品质及微生物数量的影响，本研究以披碱草为原料，设置P₁组（无添加剂，添加等量蒸馏水）、P₂组（添加植物乳杆菌，添加量为1×10⁶ CFU·g^-1 FW）、P₃组（添加糖蜜，添加量为20 g·kg^-1 FW）、P₄组（添加布氏乳杆菌，添加量为1×10⁶ CFU·g^-1 FW）、P₅组（添加纤维素酶，添加量为50 U·g^-1 FW），青贮60天后，测定其营养价值、发酵品质及微生物数量。不同青贮添加剂对披碱草的可溶性碳水化合物、中性洗涤纤维、酸性洗涤纤维和粗灰分含量均有显著影响（P<0.05）；添加剂显著降低了披碱草青贮饲料的pH值，乳酸含量显著提高（P<0.05），丙酸含量显著降低（P<0.05）。综合隶属函数分析表明，添加量为20 g·kg^-1 FW糖蜜时披碱草青贮品质最佳。

关键词: 披碱草, 添加剂, 青贮品质, 微生物数量

WANG Wei, ZHAO Muqier, WANG Zhi-jun, GE Gen-tu. Effects of Additives on Fermentation Quality and Microbial Quantity of Elymus dahuricus silage[J]. Acta Agrestia Sinica, 2026, 34(2): 679-686.

王伟, 赵牧其尔, 王志军, 格根图. 添加剂对披碱草青贮发酵品质及微生物数量的影响[J]. 草地学报, 2026, 34(2): 679-686.

References

[1] YANG J D，QI J，JIA Y W，et al. Comprehensive evaluation of productivity performance of ten wild Elymus accessions[J]. Grassland and Turf，2022，42（5）：54-61 杨娟弟，祁娟，贾燕伟，等. 10个披碱草属野生植物材料生产性能综合评价[J]. 草原与草坪，2022，42（5）：54-61
[2] SU Z G，LONG J T，GAO G R，et al. Effects of low temperature and drought stress on seed germination offour Elymus L.species[J]. Heilongjiang Animal Science and Veterinary Medicine，2024（14）：88-94 苏志刚，龙建廷，高国荣，等. 低温和干旱胁迫对4种披碱草属种子萌发的影响[J]. 黑龙江畜牧兽医，2024（14）：88-94
[3] HE YT，HU Y，DUAN H R，et al. Comprehensive evaluation of cold resistance of four Elymus forage varieties at the seedling stage[J]. Pratacultural Science，2024，41（10）：2377-2388 何永涛，胡宇，段慧荣，等. 披碱草属4个牧草品种苗期抗寒性综合评价[J]. 草业科学，2024，41（10）：2377-2388
[4] XIE LL，WANG XL，ZHOU XB，et al. Study on the Adaptability of Five Forage Grass Speciesin the Degraded Grassland in“Black Soil Hill”in the Three Rivers Headwaters region[J].Acta Agrestia Sinica，2023，31（10）：3094-3102 谢乐乐，王晓丽，周选博，等. 三江源区“黑土山”退化草地5种披碱草属牧草适应性研究[J]. 草地学报，2023，31（10）：3094-3102
[5] HE Y T，HU Y，DUAN H R，et al. Comprehensive Evaluation of drought resistance of four Elymus varietie sat seedling stage[J].Chinese Journal of Grassland，2023，45（1）：77-87 何永涛，胡宇，段慧荣，等. 披碱草属4个牧草品种苗期抗旱性综合评价[J]. 中国草地学报，2023，45（1）：77-87
[6] DING Z T，BAI J，XU D M，et al. Microbial community dynamics and natural fermentation profiles of ensiled alpine grass elymus nutans prepared from different regions of the Qinghai-Tibetan Plateau[J]. Frontiers in Microbiology，2020（11）：855
[7] LIU Y C，LI Y Y ，LU Q，et al. Effects of lactic acid bacteria additives on the quality，volatile chemicals and microbial community of leymus chinensis silage during aerobic exposure[J]. Frontiers in Microbiology，2022，13：938153
[8] WANG Y， YU Z. Effect of different additives on quality of Elymus dahuricus turcz silage[J].China Dairy Cattle，2010（7）：21-25 王莹，玉柱. 不同添加剂对披碱草青贮发酵品质的影响[J]. 中国奶牛，2010（7）：21-25
[9] YU H R，HU R C，JIA Y S，et al. Novel mechanistic understanding that Lactiplantibacillus plantarum is morecapable of improving the ensiling performance of wheat straw silage than xylanase by driving certain key metabolites[J]. Chemical and Biological Technologies in Agriculture，2024（11）：155
[10] ZHANG J Y，MA H X，GUO Q，et al. Modulation of the microbial community and the fermentation characteristics of wrapped natural grass silage inoculated with composite bacteria[J].Chemical and Biological Technologies in Agriculture，2025（12）：50
[11] Du S，XU LJ，JIANG C，et al. Novel strategy to understand the bacteria-enzyme synergy action regulates the ensiling performance of wheat straw silage by multi-omics analysis[J].International Journal of Biological Macromolecules，2025（289）：138864
[12] BAO J，ZHAO M Q E，WANG Z J，et al.Effect of Silage Additives and Ensiling Duration on Forage Millet Silage Quality[J].Chinese Journal of Grassland，2023，45（5）：88-95 包健，赵牧其尔，王志军，等. 青贮添加剂和青贮时间对饲用谷子青贮品质的影响[J]. 中国草地学报，2023，45（5）：88-95
[13] NI K K，WANG F F，ZHU BG，et al. Effects of lactic acid bacteria and molasses additives on the microbial community and fermentation quality of soybean silage[J]. Bioresource Technology，2017，238，706-715
[14] JIA Y S，YU Z， LI C F. Forage Product Quality Testing[M]. Beijing： China Agricultural University Press，2011：23-40 贾玉山，玉柱，李存福. 草产品质量检测学[M].北京：中国农业大学出版社，2011：23-40
[15] AIB V P B. Methods for dietary fiber，neutral detergent fiberand non-Starch polysaccharides in relation to animal[J].Nutrition，1991（74）：3583-3597
[16] GAO W J，LUO X N，ZHANG J J，et al. Effects of different bacterial additives on the fermentation quality of mixed silage of oilseed rape and rice straw[J]. Chinese Journal of Grassland，2025，47（1）：141-148 高文婧，罗雪宁，张佳俊，等. 不同添加菌剂对油菜与水稻秸秆混合青贮发酵品质的影响[J]. 中国草地学报，2025，47（1）：141-148
[17] KUANG Z Y，MU L，WEI L，et al. Effects of different mixture ratios and lactic acid bacteria on the quality andaerobic stability of mixed silage made from whole maize （Zea mays） and soybean（Glycine max）plants[J].Acta Prataculturae Sinica，2025，34（6）：227-238 匡宗洋，穆麟，魏岚，等.不同混合比例和乳酸菌添加对全株玉米和大豆混合青贮品质及有氧稳定性的影响[J].草业学报，2025，34（6）：227-238
[18] LIANG X Y，JJ Y，HU Y B，et al. Effects of mixing ratio and additives on the mixed silage quality of chicory and hybrid elephant grass[J]. Pratacultural Science，2025，42（1）：225-234 梁小玉，季杨，胡远彬，等. 菊苣与杂交象草混合比例和添加剂对混贮品质的影响[J]. 草业科学，2025，42（1）：225-234
[19] LI S M，ZHANG L，YAN Y F，et al.Effects of co-inoculation of cellulose decomposition bacteria and homogenous lactic acid bacteria on fermentation quality，aerobic stability，enzyme activity and ruminal degrad ability of silage maize[J]. Chinese Journal of Animal Nutrition，2024，36（11）：7185-7195 李双明，张力，闫云峰，等. 纤维素分解菌与同质型乳酸菌联合接种对青贮玉米发酵品质、有氧稳定性、酶活性和瘤胃降解率的影响[J]. 动物营养学报，2024，36（11）：7185-7195
[20] YOU S H，DU S，ZHOU Z Y，et al. Mixed Silage of sorghum hybrid sudan grass and perennial ryegrass with different proportions[J]. Acta Agrestia Sinica，2021，29（4）：821-827 尤思涵，都帅，周忠义，等. 高丹草与黑麦草适宜混贮比例研究[J]. 草地学报，2021，29（4）：821-827
[21] MA X J，ZUO Y Z，LIU R G，et al. Evaluation of productive performance and nutritional value of 20 silage maize varieties in Southeastern Gansu[J]. Acta Agrestia Sinica，2024，32（8）：2620-2629 马学军，左愈臻，刘瑞刚，等. 20个青贮玉米品种在陇东南地区的生产性能和营养价值评价[J]. 草地学报，2024，32（8）：2620-2629
[22] YANG F Y. Silage Microorganisms： An eternal topic in the feed fermentati on process[J]. Biotechnology Bulletin，2021，37（9）：1-2 杨富裕. 青贮微生物-饲料发酵过程中永恒的话题[J]. 生物技术通报，2021，37（9）：1-2
[23] XIAO S H，YUAN X J，DONG Z H，et al. Effects of inoculant and molasses additives on fermentation quality of mixed silages of tall fescue Festuca arundinacea Schreb．）and common vetch（ Vicia sativa L．）[J]. Journal of Nanjing Agricultural University，2016，39（6）：1017-1022 肖慎华，原现军，董志浩，等. 添加乳酸菌制剂和糖蜜对箭筈豌豆和苇状羊茅混合青贮发酵品质的影响[J]. 南京农业大学学报，2016，39（6）：1017-1022
[24] HU Y B，LIANG X Y，YI J，et al. Effects of mixing ratio and additives on the quality of Napier grass and whole-plant corn mixed silage[J]. Pratacultural Science，2022，39（4）：778-786 胡远彬，梁小玉，易军，等. 混合比例和添加剂对象草和全株玉米混合青贮品质的影响[J]. 草业科学，2022，39（4）：778-786
[25] ZHUANG Y F，ZHANG W C，CHEN X Z，et al. Effect of fermented green juice， cellulase and mixture on quality of water hyacinth silage[J]. Chinese Agricultural Science Bulletin，2008（5）： 35-38 庄益芬，张文昌，陈鑫珠，等. 绿汁发酵液、纤维素酶及其混合物对水葫芦青贮品质的影响[J]. 中国农学通报，2008（5）：35-38
[26] LU G C，XU H，YU Y X，et al. Effects of different additives and densities on silage quality of hybrid Pennisetum[J]. Pratacultural Science，2021，38（11）：2191-2199. 路桂聪，许辉，玉永雄，等. 不同添加物和密度对杂交狼尾草青贮效果的影响[J]. 草业科学，2021，38（11）：2191-2199
[27] WANG Q D，WANG R X，WANG C Y，et al. Effects of cellulase and Lactobacillus plantarum on fermentation quality，chemical composition，and microbial community of mixed silage of whole-plantcornand peanut vines[J]. Applied Biochemistry and Biotechnology，2022，194（6）：2465-2480
[28] REN H W，LIU F F，WANG L，et al. Effects of cellulase on the mixed silage quality of dried maize straw andcabbage waste and an analysis of its bacterial community[J]. Chinese Journal of Applied and Environmental Biology，2018，24（5）：1097-1106 任海伟，刘菲菲，王莉，等. 纤维素酶对干玉米秸秆/白菜废弃物混贮品质的影响及细菌群落解析[J]. 应用与环境生物学报，2018，24（5）：1097-1106
[29] WU C R，DAI S，LIANG L F，et al. Effects of different additives on fermentation quality and protein degradation of Broussonetia papyrifera silage[J]. Acta Prataculturae Sinica，2021，30（10）：169-179 吴长荣，代胜，梁龙飞，等. 不同添加剂对构树青贮饲料发酵品质和蛋白质降解的影响[J]. 草业学报，2021，30（10）：169-179
[30] ZHU L，WANG Z J，BAO J，et al. Effects of different low-temperature tolerant lactic acid bacteria additives on natural pasture silage fermentation[J]. Acta Agrestia Sinica，2024，32（11）：3610-3618 珠拉，王志军，包健，等. 不同耐低温乳酸菌添加剂对天然牧草青贮品质的影响[J]. 草地学报，2024，32（11）：3610-3618
[31] YANG Q，SU J Q，ZHANG S Q，et al. Effects of two lactic acid bacteria additives on quality and microflora of whole plant corn silage[J]. Chinese Journal of Animal Nutrition，2025，37（4）：2772-2787 杨琪，苏嘉琪，张思琦，等. 2种乳酸菌添加剂对全株玉米青贮品质及其微生物区系的影响[J].动物营养学报，2025，37（04）：2772-2787
[32] JU J，WEI M L，YU P，et al. Effects of Lactobacillus plantarum on fermentation quality and microbial diversity of caragana silag[J]. Acta Agrestia Sinica，2023，45（2）：113-120 巨纪，魏曼琳，于萍，等. 植物乳杆菌对柠条青贮发酵品质和微生物多样性的影响[J].中国草地学报，2023，45（2）：113-120
[33] HUANG F，ZHANG L，ZHOU B，et al. Research process in silage microorganism and its effect on silage aerobic stability[J]. Chinese Journal of Animal Nutrition，2019，31（1）：82-89 黄峰，张露，周波，等. 青贮微生物及其对青贮饲料有氧稳定性影响的研究进展[J]. 动物营养学报，2019，31（1）：82-89
[34] TANG Z H，YANG C J，LI M W，et al. Effect of applying Lactobacillus plantarum and Lactobacillus buchneri on quality and aerobic stability of sugarcane tops silage[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine，2018，45（7）：1824-1832 唐振华，杨承剑，李孟伟，等. 植物乳杆菌、布氏乳杆菌对甘蔗尾青贮品质及有氧稳定性的影响[J]. 中国畜牧兽医，2018，45（7）：1824-1832
[35] WANG H R，GAO F Q，XUE Y L，et al. Effects of different lactic acid bacteria additives on the quality and microbial diversity of oat silage[J]. Acta Agrestia Sinica，2024，46（11）：82-90 王昊然，高凤芹，薛艳林，等. 不同乳酸菌添加剂对燕麦青贮品质和微生物多样性的影响[J]. 中国草地学报，2024，46（11）：82-90
[36] JIA D W，CAO Y. Research progress of additives for high quality and safe silage[J]. Feed Industry，2024，45（12）：53-58 贾冬文，曹阳. 优质安全青贮饲料用添加剂研究进展[J]. 饲料工业，2024，45（12）：53-58
[37] LI Y，YANG Y， WANG Z N，et al. Application of lactic acid bacteria in livestock and poultry nutrition and feed[J]. Feed Industry，2024，45（5）：16-22 李杨，杨燕，王振南，等. 乳酸菌在畜禽营养与饲料中的应用[J]. 饲料工业，2024，45（5）：16-22
[38] WANG X L， FENG G Z， ZHANG C R， et al. Effects of lactic acid bacteria and cellulase on fermentation quality， aerobic stability andin vitro fermentation parameters of potato residue and corn straw mixed silage with different proportion[J]. Chinese Journal of Animal Science，2022，58（4）： 201-207 王啸林，冯冠智，张城瑞，等. 乳酸菌和纤维素酶对马铃薯渣与玉米秸秆混合青贮发酵品质、有氧稳定性和体外发酵参数的影响[J]. 中国畜牧杂志，2022，58（4）：201-207

Effects of Additives on Fermentation Quality and Microbial Quantity of Elymus dahuricus silage

添加剂对披碱草青贮发酵品质及微生物数量的影响

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[15]	DONG Lin, LIU Xiao-jing, LIN Fang, WANG Xue, WANG Jing. Study on Quality of Mixed Silage under Bean/Gramineae Forage Intercropping [J]. Acta Agrestia Sinica, 2024, 32(2): 620-629.