Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (5): 1622-1631.DOI: 10.11733/j.issn.1007-0435.2026.05.006

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Responses of Plant Multi-element Stoichiometric Characteristics of Leguminous and Gramineous Forages to Mixed Sowing

LIU Lei1, LIU Gui-he2, TAN Qi-qi1   

  1. 1. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
    2. Hebei North University, Zhangjiakou, Hebei Province 075000, China
  • Received:2025-06-16 Revised:2025-10-28 Published:2026-05-08

豆禾饲草多元素化学计量特征对混播的响应

刘蕾1, 刘贵河2, 谭琦亓1   

  1. 1. 中国农业大学草业科学与技术学院, 北京 100193;
    2. 河北北方学院, 河北 张家口 075000
  • 通讯作者: 谭琦亓，E-mail:cautanqiqi133@cau.edu.cn
  • 作者简介:刘蕾（2000-），女，汉族，安徽铜陵人，硕士研究生，主要从事草地生态与管理方向研究，E-mail:2661498893@qq.com；
  • 基金资助:
    国家自然科学基金项目（32301498）；财政部和农业农村部：国家现代农业产业技术体系（CARS-34）资助

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Abstract: Multi-element stoichiometric coupling is critical for plant growth. To investigate the effects of mixed cultivation of leguminous and gramineous forages on their multi-element stoichiometric characteristics in the Bashang agro-pastoral ecotone， this study used the monoculture of Medicago sativa and Bromus inermis as the control treatment and further analyzed the influences of two mixed sowing combinations， i.e.， M. sativa+B. inermis （MW） and M. sativa+B. inermis+Elymus nutans （MWP）， on plant nitrogen and phosphorus stoichiometric characteristics， macro-element （potassium， calcium， magnesium， sulfur） contents， and microelement （iron， manganese， copper， zinc， nickel） contents. The results showed that the nitrogen and phosphorus contents of M. sativa and B. inermis in the monoculture treatment were higher than those in the MW and MWP mixed sowing treatments. The ratio of nitrogen to phosphorus （N∶P ratio） of M. sativa in the monoculture treatment showed no difference compared to those in the MW and MWP mixed sowing treatments （all exceed 16）， indicating the phosphorus limitation of M. sativa. However， the N∶P ratio of B. inermis in the monoculture treatment was lower than those in the MW and MWP mixed sowing treatments， indicating a shift from nitrogen limitation to phosphorus limitation or dual limitation of nitrogen and phosphorus of B. inermis. Except for the zinc content of B. inermis， no differences in the macroelement and microelement contents of M. sativa and B. inermis were observed between monoculture treatments and mixed sowing treatments. In conclusion， nitrogen and phosphorus limitations might still exist in the leguminous and gramineous mixed sowing grassland in the Bashang agro-pastoral ecotone.

Key words: Artificial Grassland, Mixed sowing grassland, Ecological Stoichiometry, Macroelement, Microelement

摘要： 多元素化学计量耦合对植物生长至关重要。为探究坝上农牧交错区豆禾饲草混播对其多元素化学计量特征的影响，本研究以紫花苜蓿和无芒雀麦单播为对照，分析紫花苜蓿+无芒雀麦（MW）和紫花苜蓿+无芒雀麦+垂穗披碱草（MWP）两种混播组合对植物氮磷化学计量特征、大量元素（钾、钙、镁、硫）含量以及微量元素（铁、锰、铜、锌、镍）含量的影响。结果表明：紫花苜蓿和无芒雀麦单播处理的氮磷含量高于MW及MWP混播处理。紫花苜蓿的氮磷比在单播与MW及MWP混播处理之间无差异，但均大于16，表现为磷限制；无芒雀麦单播处理的氮磷比低于MW及MWP混播处理，表现为从氮限制转变为磷限制或氮磷共同限制。除无芒雀麦的锌含量之外，紫花苜蓿和无芒雀麦的大量及微量元素含量在单播与MW及MWP混播处理之间均无显著性差异。综上，坝上农牧交错区豆禾混播草地可能仍存在氮磷限制。

关键词: 人工草地, 混播草地, 生态化学计量学, 大量元素, 微量元素

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