Abstract:NAC gene family encode a kind of plant specific important transcriptional factors,which are involved extensively in plant growth,development and organogenesis and stress responses. However,up to date,little is known about NAC genes in Bothriochloa ischaemum. Hence,by utilizing the bioinformatics method,we analyzed the physical and chemical characteristics of encoding proteins,conserved motif,subcellular localization,potential phosphorylation sites and evolutionary relationship of 21 NAC family protein sequence from B. ischaemum. The result showed that these 21 amino acid sequences could be clustered into ten groups. The secondary structure of NAC genes was mainly random coil,containing 5 conserved motifs. Most of the NAC proteins were located in the nucleus,and potential phosphorylation sites analysis indicated that all NAC proteins contain Ser,Thr and Tyr phosphorylation sites.These results of this investigation could definitely provide a significant foundation for further research on the functional analysis of Bothriochloa ischaemum NAC gene family.
Gong X Q,Liu J H. Genetic transformation and genes for resistance to abiotic and biotic stresses in citrus and its related genera[J]. Plant Cell Tissue Organ Culture,2013,113(2):137-147
[2]
Gong X Q,Hu J B,Liu J H. Cloning and characterization of FcWRKY40,A WRKY transcription factor from Fortunella crassifolialinked to oxidative stress tolerance[J]. Plant Cell Tissue Organ Culture,2014,119(1):197-210
[3]
Liu J H,Peng T,Dai W. Critical cis-acting elements and interacting transcription factors:key players associated with abiotic stress responses in plants[J]. Plant Molecular Biology Reporter,2014,32(2):303-317
[4]
Seo D H,Ryu M Y,Jammes F,et al. Roles of four Arabidopsis U-Box E3 ubiquitin ligases in negative regulation of abscisic acid-mediated drought stress responses[J]. Plant Physiology,2012,160(1):556-568
Swati P,Pranav P S,Prem S S,et al. NAC proteins:regulation and role in stress tolerance[J]. Trends in Plant Science,2012,17(6):369-381
[7]
Ooka H,Satoh K,Doi K,et al.Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana[J]. DNA Research,2003,10(6):239-247
[8]
Tran L S,Rie N,Yamaguchi-Shinozaki K,et al. Potential utilization of NAC transcription factors to enhance abiotic stress tolerance in plants by biotechnological approach[J]. GM Crops,2010,1(1):32-39
[9]
Tran L S P,Quach S K,Guttikonda D L,et al. Molecular characterization of stress-inducible GmNAC genes in soybean[J]. molecular genetics and genomics,2009,281(6):647-664
[10]
Le D T,Nishiyama R,Watanabe Y,et al. Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in Soybean during development and dehydration stress[J]. DNA Research,2011,18(4):263-276
Su H,Zhang S,Yuan X,et al. Genome-wide analysis and identification of stress-responsive genes of the NAM-ATAF1,2-CUC2 transcription factor family in apple[J]. Plant Physiology & Biochemistry,2013,71(2):11-21
[14]
Ye G S,Ma Y H,Feng Z P,et al. Transcriptomic analysis of drought stress responses of sea buckthorn(Hippophae rhamnoides subsp.sinensis)by RNA-Seq[J]. PLoS One,2018,13(8):e202213
[15]
Rushton P J,Bokowiec M T,Han S,et al,Tobacco transcription factors:novel insights into transcriptional regulation in the Solanaceae[J]. Plant Physiology,2008,147(1):280-295
[16]
Hu R,Qi G,Kong Y,et al. Comprehensive analysis of NAC domain transcription factor gene family in Populus trichocarpa[J]. BMC Plant Biology,2010,10(1):145
[17]
Wu Z Y,Xu X Q,Xiong W D,et al. Genome-wide analysis of the NAC gene family in physic nut (Jatropha curcas L.)[J]. PLoS One,2015,10(6):124-132
[18]
Zhong R,Lee C,McCarthy R L,et al. Transcriptional activation of secondary wall biosynthesis by rice and maize NAC and MYB transcription factors[J]. Plant and Cell Physiology,2011,52(10):1856-1871
[19]
Aida M,Ishida T,Fukaki H,et al. Genes involved in organ separation in Arabidopsis:an analysis of the cup-shaped cotyledon mutant[J]. Plant Cell,1997,9(1):841-857
[20]
Raman S,Greb T,Peaucelle A,et al. Theres,Interplay of miR164, cup-shaped cotyledon genes and lateral suppressor controls axillary meristem formation in Arabidopsis thaliana[J]. Plant Journal,2008,55(1):65-76
[21]
Guo Y,Gan S. AtNAP,a NAC family transcription factor,has an important role in leaf senescence[J]. Plant Journal,2006,46(4):601-612
[22]
Liu Y,Zhang H,Xin D W,et al. Domain analysis and function prediction of TCP transcription factors family in soybean[J]. Soybean Science,2012,31(5):707-717
[23]
Kumar S,Stecher G,Tamura K. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution,2016,33(7):1870-1874
[24]
Li L,Xu H L,Yang X L,et al. Genome-wide identification,classification and expression analysis of LEA gene family in soybean[J]. Scientia Agricultura Sinica,2011,44(19):3945-3954
[25]
Xiang G S,Wang K J,Yan H J,et al. Bioinformatics analysis of MLO protein family in rosaceae plants[J]. Genomics and Applied Biology,2018,37(5):2043-2059
[26]
Chen Y,Sun X,Hu S L,et al. Analysis of NAC transcription transcription factor and its domain with secondary growth in Arabidopsis thaliana[J]. Journal of Northwest University,2009,37(5):185-194
Horton P,Park K J,Obayashi T,et al. Wolf psort:protein localization predictor[J]. Nucleic Acids Research,2007,35:585-587
[29]
Yu C S,Lin C J,Hwang J K. Predicting subcellular localization of proteins for gram-negative bacteria by support vector machines based on N-peptide compositeons[J]. Protein Science,2004,13(6):1402-1406
[30]
Emanuelsson O,Nielsen H,Brunak S,et al. Predicting Subcellular Localization of Proteins Based on their N-terminal Amino Acid Sequence[J]. Journal of Molecular Biology,2000,300(4):1005-1016
[31]
刘佳鑫. 葡萄NAC转录因子的表达及生物信息学分析[D]. 大连:辽宁师范大学,2014:9
[32]
Bailey T L,Elkan C. The value of prior knowledge in discovering motifs with MEME[J]. Proceedings of the International Conference on Intelligent Systems for Molecular Biology,1995,3:21-29
Dossa K,Xin W,Li D,et al. Insight into the AP2/ERF transcription factor superfamily in sesame and expression profiling of DREB subfamily under drought stress[J]. MBC Plant Biology,2016,16(1):171-186
[41]
Wang B,Guo X,Wang C,et al. Identification and characterization of plant-specific NAC gene family in canola (Brassica napus L.) reveal novel members involved in cell death[J]. Plant Molecular Biology,2015,87(4):395-411
[42]
Niu F,Wang B,Wu F,et al. Canola (Brassica napus L.) NAC103transcription factor gene is a novel player inducing reactive oxygen species accumulation and cell death in plants[J]. Biochemical and Biophysical Research Communications,2014,454(1):30-35
[43]
Chen Y,Qiu K,Kuai B,et al. Identification of an NAP-like transcription factor BeNAC1 regulating leaf senescence in bamboo (Bambusa emeiensis ‘Viridiflavus’)[J]. Physiologia Plantarum,2011,142(4):361-371
Duan A,Yang X,Feng K,et al. Genome-wide analysis of NAC transcription factors and their response to abiotic stress in celery (Apium graveolens L.)[J]. Computational Biology and Chemistry,2020,84:107186
[46]
Zhang J,Li L,Huang L,et al. Maize NAC-domain retained splice variants act as dominant negatives to interfere with the full-length NAC counterparts[J]. Plant Science,2019,289:110256
[47]
Shang X G,Yu Y J,Zhu L J,et al. A cotton NAC transcription factor GhirNAC2plays positive roles in drought tolerance via regulating ABA biosynthsis[J]. Plant Science,2020,296:110498