白羊草叶片和根系干旱胁迫下microRNAs差异表达分析

doi:10.11733/j.issn.1007-0435.2019.03.003

草地学报 ›› 2019, Vol. 27 ›› Issue (3): 539-546.DOI: 10.11733/j.issn.1007-0435.2019.03.003

白羊草叶片和根系干旱胁迫下microRNAs差异表达分析

李春艳^1,2, 董洁³, 钟华¹, 董宽虎¹

1. 山西农业大学动物科技学院, 山西太谷 030801;
2. 山西省农业科学院畜牧兽医研究所, 山西太原 030032;
3. 北京市科学技术情报研究所, 北京 100044

收稿日期:2018-12-02 修回日期:2019-04-15 出版日期:2019-06-15 发布日期:2019-07-19
通讯作者: 董宽虎
作者简介:李春艳(1978-),女,山西岚县人,硕士,副研究员,主要从事牧草遗传育种研究,E-mail:licyjzgjkx@163.com
基金资助:
教育部高等学校博士学科点专项科研基金项目（20101403110002）；山西省科技攻关项目（20120311011-1）；山西省科技基础条件平台建设项目（2012091004-0101）资助

MicroRNAs Expressions Profiling of Bothriochloa ischaemum Leaves andRoots under Drought Stress

LI Chun-yan^1,2, DONG Jie³, ZHONG Hua¹, DONG Kuan-hu¹

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. Institute of Animal Husbandry and Veterinary, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi Province 030032, China;
3. Beijing Science and Technology Information Institute, Beijing 100044, China

Received:2018-12-02 Revised:2019-04-15 Online:2019-06-15 Published:2019-07-19

摘要/Abstract

摘要： 为了探索干旱胁迫下白羊草miRNAs的组织特异性，本研究在干旱胁迫下白羊草（Bothriochloa ischaemum）转录组数据的基础上，采用Illumina测序技术和生物信息学方法，对干旱胁迫和正常生长条件下白羊草苗期叶片和根系miRNAs（microRNAs）进行鉴定和表达分析研究。结果从4个库中鉴定出白羊草中有属于20个家族的79个已知miRNAs和92个新miRNAs。干旱胁迫后叶片和根系中差异表达的miRNAs分别有65个（上调28和下调37）和27个（上调15和下调12）。响应干旱胁迫的主要miRNAs为miR156，miR164，miR166，miR169，miR172，miR396，miR398，miR408，miR528。对这些miRNAs预测的靶基因进行GO生物功能和KEGG代谢通路富集分析表明，植物激素信号转导是叶片和根系响应干旱胁迫共同富集的主要代谢通路，而黄酮类化合物生物合成则是干旱胁迫下叶片富集的最主要代谢通路。

关键词: 白羊草, 干旱胁迫, MiRNA, 生物信息学

Abstract: To investigate the tissue-specific miRNAs response to drught stress in Bothriochloa ischaemum. Based on the transcriptome data of Bothriochloa ischaemum under drought stress,we used Illumina sequencing and bioinformatics methods to identify and analyze the expression of miRNAs in leaves and roots of Bothriochloa ischaemum seedlings under drought stress and normal growth conditions. Seventy-nine Known miRNAs belonging to 20 families and 92 Novel miRNAs were identified from four libraries. There were 65 miRNAs (28 upregulated and 37 downregulated) and 27 miRNAs (15 upregulated and 12 downregulated) were differentially expressed in leaves and roots after drought stress,respectively. The major miRNAs responding to drought stress were miR156,miR164,miR166,miR169,miR172,miR396,miR398,miR408 and miR528. The enrichment analysis of GO biological function and KEGG metabolic pathway showed that plant hormone signal transduction was the main metabolic pathway in response to drought stress in leaves and roots,and flavonoid biosynthesis was the major rich factor for Bothriochloa ischaemum leaves responding to drought stress.

Key words: Bothriochloa ischaemum, Drought stress, MiRNA, Bioinformatics

中图分类号:

Q945
S543.9

李春艳, 董洁, 钟华, 董宽虎. 白羊草叶片和根系干旱胁迫下microRNAs差异表达分析[J]. 草地学报, 2019, 27(3): 539-546.

LI Chun-yan, DONG Jie, ZHONG Hua, DONG Kuan-hu. MicroRNAs Expressions Profiling of Bothriochloa ischaemum Leaves andRoots under Drought Stress[J]. Acta Agrestia Sinica, 2019, 27(3): 539-546.

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白羊草叶片和根系干旱胁迫下microRNAs差异表达分析

MicroRNAs Expressions Profiling of Bothriochloa ischaemum Leaves andRoots under Drought Stress

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