海雀稗HsFA基因家族鉴定及热胁迫应答分析

doi:10.11733/j.issn.1007-0435.2025.03.010

草地学报 ›› 2025, Vol. 33 ›› Issue (3): 766-777.DOI: 10.11733/j.issn.1007-0435.2025.03.010

• 研究论文 • 上一篇

海雀稗HsFA基因家族鉴定及热胁迫应答分析

彭迪, 陈海洋, 蔡丽蓉, 陆洋, 刘大林, 王小山, 严学兵, 潘玲

扬州大学动物科学与技术学院, 江苏扬州 225000

收稿日期:2024-04-15 修回日期:2024-05-30 发布日期:2025-04-07
通讯作者: 潘玲,E-mail:panling1199@126.com
作者简介:彭迪（2004-）,女,汉族,湖北黄冈人,本科生,主要从事海雀稗机理研究,E-mail：18252784414@163.com;陈海洋（2003-）,男,汉族,安徽宿州人,本科生,主要从事海雀稗机理研究,E-mail：c19955722274@163.com
基金资助:
海南省国家自然科学基金青年基金一项（322QN248）;扬州大学海内外优秀人才科研启动经费（137013098/5019）;国家自然科学基金（32401488）资助

Identification of HsFA Family Genes and Expression Profiling in Paspalum vaginatum under Heat Stress Conditions

PENG Di, CHEN Hai-yang, CAI Li-rong, LU Yang, LIU Da-lin, WANG Xiao-shan, YAN Xue-bing, PAN Ling

Yangzhou University, College of Animal Science and Technology, Yangzhou, Jiangsu Province 225000, China

Received:2024-04-15 Revised:2024-05-30 Published:2025-04-07

摘要/Abstract

摘要： 本研究采用生物信息学方法鉴定海雀稗（Paspalum vaginatum Sw.）中的热激转录因子PvHsFA家族基因成员,并探究其在海雀稗热胁迫应答中的调控机制,旨在提供相关理论依据。通过生物信息学手段鉴定了PvHsFA基因家族的成员,并分析这些成员的理化性质、启动子区域的顺式作用元件、构建进化树以及进行染色体定位。此外,利用海雀稗品种‘海岛2000’的RNA-seq数据对基因表达水平进行了验证。共鉴定出25个PvHsFA家族成员,其编码氨基酸数目261~521,这些蛋白大多为酸性蛋白和亲水性蛋白。启动子区域含有大量与非生物胁迫相关、植物激素相关及生长发育相关的元件。这些基因具有较高的保守性,其中21 个基因在热胁迫条件下表现出显著的差异表达。PvHsFA基因家族可能在海雀稗的热胁迫响应调控中发挥重要作用,本研究为深入揭示海雀稗的耐热机制奠定了基础。

关键词: 生物信息学, HsFA基因家族, 海雀稗, 转录因子, 热胁迫, 基因表达

Abstract: This study aims to perform a bioinformatics analysis to identify and characterize the PvHsFA gene family of heat stress transcription factors in seashore paspalum （Paspalum vaginatum Sw.）. The goal is to offer theoretical insights into the regulatory mechanisms of this gene family during the heat stress response in seashore paspalum. We employed bioinformatics tools to discover and to classify members of the PvHsFA gene family. We then analyzed their physicochemical properties, the presence of cis-acting elements within their promoter sequences, constructed evolutionary trees, and mapped their chromosomal locations. To validate our findings, RNA-sequencing data from ‘Sealsle 2000’ cultivar of seashore paspalum were utilized. Our analysis identified a total of 25 PvHsFA gene family members. The proteins varied in size, with the number of coding amino acids ranging from 261 to 521, and the majority exhibited characteristics of being acidic and hydrophilic. The promoters of these genes were rich in hormone-responsive, stress-responsive, and growth-related elements. Expression profiling indicated that 21 of the genes were actively expressed. The findings suggest that the PvHsFA gene family may play a pivotal role in mediating the heat stress response in seashore paspalum. This research establishes a foundation for future investigations into the heat tolerance mechanisms of this ecologically significant grass species.

Key words: Bioinformatics, HsFA gene family, Paspalum vaginatum, Transcription factor, Heat stress, Gene expression

中图分类号:

S688.4

彭迪, 陈海洋, 蔡丽蓉, 陆洋, 刘大林, 王小山, 严学兵, 潘玲. 海雀稗HsFA基因家族鉴定及热胁迫应答分析[J]. 草地学报, 2025, 33(3): 766-777.

PENG Di, CHEN Hai-yang, CAI Li-rong, LU Yang, LIU Da-lin, WANG Xiao-shan, YAN Xue-bing, PAN Ling. Identification of HsFA Family Genes and Expression Profiling in Paspalum vaginatum under Heat Stress Conditions[J]. Acta Agrestia Sinica, 2025, 33(3): 766-777.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2025.03.010

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2025/V33/I3/766

参考文献

[1] 陈培琴,郁松林,詹妍妮,等.植物在高温胁迫下的生理研究进展[J].中国农学通报,2006,22(5):223-227
[2] PRITI K. Plant response to heat stress[J]. Topics in Current Genetics,2004(4):73-101
[3] HUANG B R,XU C P. Identification and characterization of proteins associated with plant tolerance to heat stress[J]. Journal of Integrative Plant Biology,2008,50(10):1230-1237
[4] NOVER L,BHARTI K,DÖRING P,et al. Arabidopsis and the heat stress transcription factor world:how many heat stress transcription factors do we need?[J]. Cell Stress& Chaperones,2001,6(3):177-189
[5] VON KOSKULL-DÖRING P,SCHARF K D,NOVER L. The diversity of plant heat stress transcription factors[J]. Trends in Plant Science,2007,12(10):452-457
[6] CHAUHAN H,KHURANA N,AGARWAL P,et al. Heat shock factors in rice (Oryza sativa L.):genome-wide expression analysis during reproductive development and abiotic stress[J]. Molecular Genetics and Genomics,2011,286(2):171-187
[7] YANG X D,ZHU W M,ZHANG H,et al. Heat shock factors in tomatoes:genome-wide identification,phylogenetic analysis and expression profiling under development and heat stress[J]. PeerJ,2016,4:e1961
[8] GUO J K,WU J,JI Q,et al. Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis[J]. Journal of Genetics and Genomics,2008,35(2):105-118
[9] MITTAL D,CHAKRABARTI S,SARKAR A,et al. Heat shock factor gene family in rice:genomic organization and transcript expression profiling in response to high temperature,low temperature and oxidative stresses[J]. Plant Physiology and Biochemistry,2009,47(9):785-795
[10] XUE G P,SADAT S,DRENTH J,et al. The heat shock factor family from Triticum aestivum in response to heat and other major abiotic stresses and their role in regulation of heat shock protein genes[J]. Journal of Experimental Botany,2014,65(2):539-557
[11] PANZADE K P,KALE S S,KAPALE V,et al. Genome-wide analysis of heat shock transcription factors in Ziziphus jujuba identifies potential candidates for crop improvement under abiotic stress[J]. Applied Biochemistry and Biotechnology,2021,193(4):1023-1041
[12] NISHIZAWA A,YABUTA Y,YOSHIDA E,et al. Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress[J]. The Plant Journal,2006,48(4):535-547
[13] CHARNG Y Y,LIU H C,LIU N Y,et al. A heat-inducible transcription factor,HsfA2,is required for extension of acquired thermotolerance in Arabidopsis[J]. Plant Physiology,2007,143(1):251-262
[14] LARKINDALE J,VIERLING E. Core genome responses involved in acclimation to high temperature[J]. Plant Physiology,2008,146(2):748-761
[15] SCHRAMM F,LARKINDALE J,KIEHLMANN E,et al. A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis[J]. The Plant Journal,2008,53(2):264-274
[16] YOSHIDA T,SAKUMA Y,TODAKA D,et al. Functional analysis of an Arabidopsis heat-shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stress-regulatory system[J]. Biochemical and Biophysical Research Communications,2008,368(3):515-521
[17] MEIRI D,BREIMAN A. Arabidopsis ROF1(FKBP62) modulates thermotolerance by interacting with HSP90.1 and affecting the accumulation of HsfA2-regulated sHSPs[J]. The Plant Journal,2009,59(3):387-399
[18] HUANG Y C,NIU C Y,YANG C R,et al. The heat stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses[J]. Plant Physiology,2016,172(2):1182-1199
[19] FRIEDRICH T,OBERKOFLER V,TRINDADE I,et al. Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis[J]. Nature Communications,2021,12(1):3426
[20] LI B J,JIANG S M,GAO L,et al. Heat shock factor A1s are required for phytochrome-interacting factor 4-mediated thermomorphogenesis in Arabidopsis[J]. Journal of Integrative Plant Biology,2024,66(1):20-35
[21] 解新明,卢小良.海雀稗种质资源的优良特性及其利用价值[J].华南农业大学学报,2004,25(Sup):64-67
[22] PAN L,HU X,LIAO L,et al. Lipid metabolism and antioxidant system contribute to salinity tolerance in halophytic grass seashore Paspalum in a tissue-specific manner[J]. BMC Plant Biology,2023,23(1):337
[23] SUN G C,WASE N,SHU S Q,et al. Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass[J]. Nature Communications,2022,13(1):7731
[24] 王曦,汪小我,王立坤,等.新一代高通量RNA测序数据的处理与分析[J].生物化学与生物物理进展,2010,37(8):834-846
[25] MCCOUCH S R. Genomics and synteny[J]. Plant Physiology,2001,125(1):152-155
[26] SONG C,WANG Y. Microsynteny analysis of tomato and peach genome[J]. Semantic Scholar,2010,32(9):966-973
[27] 邹苇鹏,翟佳兴,李迪娜,等.紫花苜蓿NAC基因家族鉴定及在非生物胁迫下的表达模式分析[J].草地学报,2024,32(8):2440-2458
[28] 陈生蓉,史国民,王乐,等.青稞BBX基因家族鉴定及其对UV-B的响应[J].草地学报,2024,32(6):1760-1769
[29] 刘宇.海滨雀稗热激转录因子PvHSFA4a调控耐镉的分子机制[D].南京:南京农业大学,2022:83-87
[30] NISHIZAWA-YOKOI A,NOSAKA R,HAYASHI H,et al. HsfA1d and HsfA1e involved in the transcriptional regulation of HsfA2 function as key regulators for the hsf signaling network in response to environmental stress[J]. Plant& Cell Physiology,2021,52(5):933-945
[31] 张楠,王映红,王志敏,等.植物热激转录因子家族的研究进展[J].生物工程学报,2021,37(4):1155-1167

海雀稗HsFA基因家族鉴定及热胁迫应答分析

Identification of HsFA Family Genes and Expression Profiling in Paspalum vaginatum under Heat Stress Conditions

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘莉婷, 高佳淼, 周鸿明, 李季肤, 王志勇, 陈志坚. 地毯草扩张蛋白基因AcEXPA1的克隆与表达分析[J]. 草地学报, 2025, 33(2): 351-359.
[2]	王越, 郭璟, 张银翠, 李静, 祁存英, 周同永. 燕麦AsDof6.5基因的克隆及其在低氮胁迫下的表达分析[J]. 草地学报, 2025, 33(2): 360-369.
[3]	曲荣举, 刘玉萍, 苏旭, 富贵, 靳佳瑞, 杨倩, 张朋辉, 余明君, 孙成林, 毛轩睿. 基于PacBio平台的扇穗茅全长转录组测序及热激转录因子家族（HSFs）分析[J]. 草地学报, 2025, 33(2): 370-381.
[4]	罗李旋, 周涛, 徐倩, 卢蕊, 刘宁芳, 胡龙兴. 美洲狼尾草GATA转录因子家族生物信息学分析[J]. 草地学报, 2025, 33(2): 391-400.
[5]	杜红旗, 闫祥洲, 冯长松, 韩康康, 吕先召, 娄治国, 刘磊, 梁志妍. RBP47C调控苜蓿生长的分子机理研究[J]. 草地学报, 2025, 33(2): 401-409.
[6]	何军刚, 李昕蓉, 魏小成, 谢耀慧, 谢鑫明, 李成义. 红芪HpC4H基因的克隆及其铁盐干预下的表达分析[J]. 草地学报, 2024, 32(9): 2749-2758.
[7]	马晓璇, 梁程博, 刘道鑫, 孙国, 闫京艳. 西氏熊蜂线粒体基因组结构特征及系统发育分析[J]. 草地学报, 2024, 32(8): 2408-2418.
[8]	邹苇鹏, 翟佳兴, 李迪娜, 黄洁琼, 郭康杰, 岑慧芳, 朱慧森, 许涛. 紫花苜蓿NAC基因家族鉴定及在非生物胁迫下的表达模式分析[J]. 草地学报, 2024, 32(8): 2440-2458.
[9]	钱晚青, 安聪, 庄黎丽. 高羊茅赤霉素受体家族成员鉴定及其在不同逆境下的表达模式分析[J]. 草地学报, 2024, 32(6): 1719-1728.
[10]	刘筠, 李效雄, 贾西贝, 马瑞, 柳迪, 杜雨, 胡晓桐, 马彦军. 黑果枸杞YABBY转录因子家族鉴定与生物信息学分析[J]. 草地学报, 2024, 32(6): 1729-1741.
[11]	陈生蓉, 史国民, 王乐, 何涛. 青稞BBX基因家族鉴定及其对UV-B的响应[J]. 草地学报, 2024, 32(6): 1760-1769.
[12]	于元平, 蒋宇佳, 孙向一, 吴春妍, 周敏, 刘明稀. 假俭草WRKY家族基因鉴定及其响应干旱胁迫表达分析[J]. 草地学报, 2024, 32(5): 1378-1391.
[13]	刘占玲, 寇桂香, 南彦斌, 王克鑫, 周渊涛. 青海草原毛虫IR基因的鉴定及组织表达分析[J]. 草地学报, 2024, 32(5): 1392-1400.
[14]	朱晨晨, 史昆, 何沁坤, 郑雨琴, 苑峰, 刘亚玲, 王赞. 混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响[J]. 草地学报, 2024, 32(4): 1044-1054.
[15]	荣梦茹, 余如刚, 韦英铭, 王国良, 杜雪玲, 杨改梅, 高佳佳. 紫花苜蓿耐盐性相关NAC转录因子的挖掘及表达分析[J]. 草地学报, 2024, 32(4): 1055-1067.