混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响

doi:10.11733/j.issn.1007-0435.2024.04.007

草地学报 ›› 2024, Vol. 32 ›› Issue (4): 1044-1054.DOI: 10.11733/j.issn.1007-0435.2024.04.007

• 研究论文 • 上一篇

混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响

朱晨晨¹, 史昆¹, 何沁坤¹, 郑雨琴¹, 苑峰², 刘亚玲², 王赞¹

1. 中国农业大学草业科学与技术学院, 北京 100193;
2. 内蒙古草业技术创新中心有限公司, 内蒙古呼和浩特 010070

收稿日期:2024-01-12 修回日期:2024-03-20 发布日期:2024-05-07
通讯作者: 王赞,E-mail:zanwang@cau.edu.cn
作者简介:朱晨晨(1996-),女，汉族，山东聊城人，博士研究生，主要从事牧草基因资源挖掘与利用的相关研究，E-mail:zhucc@cau.edu.cn
基金资助:
国家草业技术创新中心(筹)重大创新平台建设专项(CCPTZX2023B04)；国家林业和草原种质资源库(2005DKA21003)资助

Effects of Mixed Saline-Alkali Stress on Physiology and Gene Expression of Alfalfa Seedlings

ZHU Chen-chen¹, SHI Kun¹, HE Qin-kun¹, ZHENG Yu-qin¹, YUAN Feng², LIU Ya-ling², WANG Zan¹

1. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
2. Inner Mongolia Pratacultural Technology Innovation Center Co., Ltd, Hohhot, Inner Mongolia 010070, China

Received:2024-01-12 Revised:2024-03-20 Published:2024-05-07

摘要/Abstract

摘要： 本研究以'ZN-1’和'保定’苜蓿(Medicago sativa L.'Baoding’)为试验材料，以浓度梯度为0 mmol·L^-1，40 mmol·L^-1和80 mmol·L^-1，pH值为9.3的混合盐碱溶液(NaCl∶Na₂SO₄∶NaHCO₃∶Na₂CO₃摩尔比为1∶1∶1∶1)进行胁迫处理，比较2个紫花苜蓿幼苗的生长指标、生理指标和基因表达(ROS清除、离子转运和光合调控3类基因)差异,以期为解析紫花苜蓿耐混合盐碱胁迫响应机制提供理论依据。结果表明，随着混合盐碱胁迫浓度的增加，2个紫花苜蓿的株高、干重、叶绿素含量、光合速率以及Fv/Fm等均出现明显的损伤(P＜0.05)。其中，'ZN-1’的耐混合盐碱性显著高于'保定’苜蓿，主要表现为'ZN-1’的株高和生物量更高、叶绿素含量和抗氧化酶的活性更高，净光合速率更强，脯氨酸和可溶性糖含量更高，叶片ROS积累量和地下部Na⁺积累更少，ROS清除、脯氨酸生物合成、Na⁺液泡区隔化以及叶绿素维持相关编码基因的表达水平更高。因此，脯氨酸生物合成、Na⁺液泡区隔化、叶绿素维持以及抗氧化酶可能在'ZN-1’耐混合盐碱胁迫中发挥重要作用。

关键词: 紫花苜蓿, 混合盐碱胁迫, 生理响应, 基因表达

Abstract: In this study,two alfalfa (Medicago sativa L.) materials 'Baoding’ and 'ZN-1’ were treated with the mixed saline-alkali solution (NaCl∶Na₂SO₄∶NaHCO₃∶Na₂CO₃ in 1∶1∶1∶1 ratio at pH 9.3) at the concentrations of 0 mmol·L^-1,40 mmol·L^-1 and 80 mmol·L^-1,and compared for the tolerance. The morphology,physiology,and expression of the selected genes that are involved in ROS-scavenging,ion transport,and photosynthetic regulation were investigated at the alfalfa seedling stage,to look into the response to mixed saline-alkali stress. The results showed that with the increase of mixed saline-alkali concentration,significant (P＜0.05) damage was observed in the plant height,dry weight,chlorophyll content,photosynthetic rate,and Fv/Fm,in both the alfalfa materials. Compared with 'Baoding’,'ZN-1’ demonstrated significantly higher saline-alkali tolerance,as evidenced by higher plant height,more biomass,more contents of chlorophyll content,proline,and soluble sugar,stronger antioxidant activity,promoted robust photosynthesis,and less accumulation of ROS in leaves and Na⁺in root. In 'ZN-1’,the significantly up-regulated genes were associated with the pathways of ROS scavenging,proline biosynthesis,Na⁺ vacuolar compartmentalization,and chlorophyll maintenance. In summary,proline biosynthesis,Na⁺ vacuolar compartmentalization,chlorophyll maintenance,and antioxidants may play an important role in the 'ZN-1’ tolerance of mixed saline-alkali stress.

Key words: Alfalfa, Mixed saline-alkali stress, Physiological responses, Genes expression

中图分类号:

S541.9

朱晨晨, 史昆, 何沁坤, 郑雨琴, 苑峰, 刘亚玲, 王赞. 混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响[J]. 草地学报, 2024, 32(4): 1044-1054.

ZHU Chen-chen, SHI Kun, HE Qin-kun, ZHENG Yu-qin, YUAN Feng, LIU Ya-ling, WANG Zan. Effects of Mixed Saline-Alkali Stress on Physiology and Gene Expression of Alfalfa Seedlings[J]. Acta Agrestia Sinica, 2024, 32(4): 1044-1054.

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混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响

Effects of Mixed Saline-Alkali Stress on Physiology and Gene Expression of Alfalfa Seedlings

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