菌物学报 ›› 2021, Vol. 40 ›› Issue (10): 2735-2751.doi: 10.13346/j.mycosystema.210279

• 研究论文 • 上一篇    下一篇

黄山典型植被类型土壤真菌群落特征及其影响因素

满百膺1,2,向兴1,罗洋1,毛小涛1,张超1,孙丙华2,*(),王希2   

  1. 1.上饶师范学院生命科学学院 江西 上饶 334001
    2.安徽省黄山生物多样性与短尾猴行为生态学国际联合研究中心 安徽 合肥 230039
  • 收稿日期:2021-07-15 接受日期:2021-08-30 出版日期:2021-10-22 发布日期:2021-11-05
  • 通讯作者: 孙丙华 E-mail:binghuasun00@126.com
  • 作者简介:ORCID: MAN Bai-Ying (0000-0002-1001-0638)
  • 基金资助:
    安徽省黄山生物多样性与短尾猴行为生态学国际联合研究中心开放课题(KF200013)

Characteristics and influencing factors of soil fungal community of typical vegetation types in Mount Huangshan, East China

MAN Bai-Ying1,2,XIANG Xing1,LUO Yang1,MAO Xiao-Tao1,ZHANG Chao1,SUN Bing-Hua2,*(),WANG Xi2   

  1. 1. College of Life Sciences, Shangrao Normal University, Shangrao, Jiangxi 334001, China
    2. International Research Center for Huangshan Biodiversity and Tibetan Macaque Behavioral Ecology, Hefei, Anhui 230039, China
  • Received:2021-07-15 Accepted:2021-08-30 Online:2021-10-22 Published:2021-11-05
  • Contact: Bing-Hua SUN E-mail:binghuasun00@126.com
  • Supported by:
    International Research Center for Huangshan Biodiversity and Tibetan Macaque Behavioral Ecology(KF200013)

摘要:

黄山地势高差明显,植被类型多样,生态系统保存完整,是研究森林生态系统土壤真菌群落的天然实验室。本研究采集黄山典型植被下土壤样本,利用Illumina NovaSeq高通量测序技术分析土壤真菌群落结构,结合土壤理化性质探讨不同植被类型影响真菌群落组成的潜在因素。结果共检测到13个真菌门,优势真菌门依次为:担子菌门Basidiomycota,获得38目,202属,相对丰度介于7.30%-90.71%,在常绿落叶阔叶混交林、山地矮林及落叶阔叶林中出现高值,局部呈现先增后减的单峰变化格局;子囊菌门Ascomycota有56目,393属,相对丰度介于4.69%-53.07%,随着典型植被类型变化无明显变化规律;被孢霉门Mortierellomycota获得1目和2属,相对丰度介于2.88%-29.92%,随着典型植被类型变化呈现U型变化模式;5种植被类型土壤中共检测到34个不同分类单元的真菌指示类群,落叶阔叶林土壤真菌指示类群最为丰富,占67%;pH显著影响土壤真菌α多样性(Pearson,P<0.001),是黄山土壤真菌群落变异的主控因子(Monte Corlo 检验,P<0.01)。

关键词: 黄山, 真菌群落结构, Illumina NovaSeq测序, 土壤pH

Abstract:

Mount Huangshan has a variety of vegetation types, well-preserved ecosystems and obvious differences in vertical geography, which provide a natural laboratory for studying the soil fungal communities in forest ecosystems. In this study, soil samples were collected from typical vegetation in Huangshan Mountain. Illumina NovaSeq high-throughput sequencing technology was used to analyze soil fungal community structure. Combined with soil physical and chemical properties, potential factors affecting fungal community composition of different vegetation types were discussed. Thirteen fungal phyla were detected, and the dominance was Basidiomycota (38 orders, 202 genera) with relative abundance ranged from 7.30% to 90.71%, showing a high value in evergreen and deciduous broad-leaved mixed forest, montane coppice and deciduous broad-leaved forest, but a single peak pattern of increasing first and then decreasing occurred in some areas. There are 393 genera in 56 orders of Ascomycota with relative abundance ranged from 4.69% to 53.07%, and there is no obvious change with the vegetation types. Two genera in one order of Mortierellomycota were obtained, with relative abundance ranged from 2.88%-29.92%, showing a U-shaped pattern with the change of vegetation types. A total of 34 fungal indicator groups of different taxa was detected in soil of five vegetation types, and 67% taxa of fungal indicator groups were detected in deciduous broad-leaved forest. pH significantly affected the α diversity of soil fungal communities (Pearson, P<0.001), which was the dominant factor affecting the variation of soil fungal community in Mount Huangshan, China (Monte Corlo Test, P<0.01).

Key words: Mount Huangshan, fungal community structure, Illumina NovaSeq sequencing, soil pH