漏斗多孔菌液体菌种培养基及其栽培条件

钟丽娟; 赵新海

doi:10.13346/j.mycosystema.210345

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菌物学报 ›› 2021, Vol. 40 ›› Issue (12) : 3118-3128. DOI: 10.13346/j.mycosystema.210345 CSTR: 32115.14.j.mycosystema.210345

研究论文

漏斗多孔菌液体菌种培养基及其栽培条件

作者信息 +

Liquid culture medium and cultivation conditions of Polyporus arcularius

Author information +

文章历史 +

摘要

为实现漏斗多孔菌资源化利用,设计单因素试验,以菌丝生物量、菌球密度和菌球直径为指标,获得漏斗多孔菌Polyporus arcularius液体菌种培养基配方为马铃薯（去皮）200g、玉米粉20.0g、蛋白胨5.0g、KH₂PO₄3.0g、K₂HPO₄ 1.0g,MgSO₄·7H₂O 1.5g,初始pH 5.0,并优化培养条件为装液量250mL/500mL,接种量2%,培养温度32℃,转速170r/min,种龄6d;依上述条件制备液体菌种,以固体菌种为对照,进行出菇试验,调查农艺性状,试验结果表明液体菌种接种后,菌袋满袋时间提前7d,子实体原基产生时间提前8d,头潮菇鲜重提高22.5%;配方试验结果表明,以细木屑为主料的配方产量最高,头潮菇鲜重为270.6g/袋。

Abstract

A single factor test was designed to determine the mycelial biomass, mycelial spheroid density and diameter of liquid culture of Polyporus arcularius. The formula of liquid medium was 200g potato, 20g corn meal, 5g peptone, 3.0g KH₂PO₄, 1.0g K₂HPO₄, and MgSO₄·7H₂O 1.5g, and the initial pH was adjusted to 5.0. Optimized culture conditions were: liquid volume of 250mL/500mL, inoculation volume of 2%, culture temperature of 32°C, speed of rotation 170r/min, inoculum aged 6d. According to the above conditions, the liquid culture was prepared. The solid culture was used as the control, and the cultivation experiment was carried out to investigate the agronomic characters. The results showed that using the liquid culture as inoculum, the fruiting stage could be shifted to earlier date, and the mycelial sackful colonization time was 7d earlier than usual. The formation of primordia was 8d earlier than usual, and the fresh weight of first harvest fruiting bodies increased by 22.5%. Using fine sawdust as main substrate could harvest the highest yield, and the harvest fresh weight of fruiting bodies could reach 270.6g/bag.

导出引用

钟丽娟, 赵新海. 漏斗多孔菌液体菌种培养基及其栽培条件[J]. 菌物学报, 2021, 40(12): 3118-3128 https://doi.org/10.13346/j.mycosystema.210345

ZHONG Li-Juan, ZHAO Xin-Hai. Liquid culture medium and cultivation conditions of Polyporus arcularius[J]. Mycosystema, 2021, 40(12): 3118-3128 https://doi.org/10.13346/j.mycosystema.210345

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]

Azuma

, Hori

, Ohashi

, Yoshida

, Horinouchi

, Beppu

, 2014. Basidifferquinone, a new inducer for fruiting-body formation of a basidiomycetes Favolus arcularius from a streptomyces strain II. Structure of Basidifferquinone, Bioscience, Biotechnology, and Biochemistry Volume, 54(6): 1447-1452

本文引用 [1]

[2]	Bao DP, Wang Y, Gao YN, Wang Y, Li Y, Tang LH, Mao WJ, Zhou CL, Gong M, Wan JN, Yang RH, Wu YY, 2019. The immunoregulatory protein Fip-par1from Favolus arcularius and its preparation method and application technology. China: CN106496311B. 2019-07-05 (in Chinese)

[3]	Cui BK, Li HJ, Ji X, Zhou JL, Song J, Si J, Yang ZL, Dai YC, 2019. Species diversity, taxonomy and phylogeny of Polyporaceae (Basidiomycota) in China. Fungal Diversity, 97: 137-392 (in Netherlands) https://doi.org/10.1007/s13225-019-00427-4 https://doi.org/10.1007/s13225-019-00427-4 本文引用 [1]

[4]	Dai YC, 2010. Species diversity of wood-decaying fungi in Northeast China. Mycosystema, 29: 801-818 (in Chinese)

[5]	Dong HR, Zhou F, Li Y, Li ZP, Jiang N, 2021. Effects of nutrient solution pH on Lentinus edodes mycelium. Shanghai Vegetables, 2021(4): 65-68 (in Chinese)

[6]	Duan YC, Hu ZY, Yang F, Li JT, Wu XL, Zhang RY, 2020. Effects of pH and buffering on the growth of Lentinula edodes mycelium. Scientia Agriculturae Sinica, 53(22): 4683-4690 (in Chinese)

[7]	Guo DZ, Ma A, Hu JJ, Li D, Zhang B, Li Y, 2019. Solid incubation, domestic cultivation and cellulase activities of Elmerina hispida. Mycosystema, 38(7): 1111-1119 (in Chinese)

[8]	Huang NL, 1998. Colored illustrstions of macrofungi (Mushroons) of China. China Agricultural Press, Beijing. 59 (in Chinese)

[9]

Kitamoto

, Akita

, Horikoshi

, 1999. Effects of high-temperature treatment on two essential light processes and an intervening dark process in photoinduced pileus primordium formation of a basidiomycete, Favolus arcularius. Mycoscience, 40(2): 103-108 (in Germany)

https://doi.org/10.1007/BF02464288

https://linkinghub.elsevier.com/retrieve/pii/S1340354099708727

本文引用 [1]

[10]	Kitamoto Y, Horikoshi T, Kasai Z, 1974. Growth of fruit-bodies in Favolus arcularius. Journal of Plant Research Volume, 87(1): 41-49 (in Germany) 本文引用 [1]

[11]	Kitamato Y, Kahashi M, Kasai Z, 1968. Light-induced formation of fruit-bodies in a basidiomycete, Favolus arcularius (Fr.) Ames. Plant and Cell Physiology, 9(4): 797-805 (in Japan) 本文引用 [1]

[12]	Li Y, 2018. The status, opportunities and challenges of edible fungi industry in China: develop with Chinese characteristics, realize the dream of powerful mushroom industrial country. Journal of Fungal Research, 16(3): 125-131 (in Chinese)

[13]	Liu N, Zhang M, Zhang SY, Li HL, Song Y, 2020. Study on optimization of cultivation conditions of liquid strains and industrial production of Lyophyllum fumosum. Edible Fungi of China, 39(10): 50-55 (in Chinese)

[14]	Liu W, Cai YL, He PX, Zhang Y, Bian YB, 2016. Morphological and structural analysis of mitospore of Morchella importuna. Journal of Fungal Research, 14(3): 157-161 (in Chinese)

[15]	Ma L, Yang C, Xiao DL, Jiang XL, Ying ZH, Lin YQ, 2020. Liquid fermentation of Sparassis latifolia on different carbon and nitrogen source combinations. Edible Fungi of China, 39(10): 37-41+45 (in Chinese)

[16]	Qi LL, An Y, Li Y, 2011. Selected biological characteristics and artificial cultivation of Oudemansiella brunneomarginata. Acta Edulis Fungi, 18(3): 35-38 (in Chinese)

[17]	Wang JF, Wang Z, Li J, Rao J, 2009. Production of laccase from Polyporus arcularius A 08 by submerged fermentation. Mycosystema, 28(3): 440-444 (in Chinese)

[18]	Wu F, Zhou LW, Yang ZL, Bau T, Li TH, Dai YC, 2019. Resource diversity of Chinese macrofungi: edible, medicinal and poisonous species. Fungal Diversity, 98: 1-76 https://doi.org/10.1007/s13225-019-00432-7 https://doi.org/10.1007/s13225-019-00432-7 本文引用 [1]

[19]	Yin W, Liu JS, Wu PY, Liang YM, Wang G, 2012. Chemical constituents of Favolus arcularius. Natural Product Research and Development, 24(12): 1747-1749, 1756 (in Chinese)

[20]	Zhang WT, Yao FJ, Fang M, Zhang YM, Ma XX, Huang CY, Zhang Y, 2021. Study on liquid culture of Hericium Coralloides and its cultivation effects. Journal of Northeast Agricultural Sciences, (2): 125-131 (in Chinese)

[21]	Zhang Y, Wang Y, Ban LT, Huang L, Zheng ZG, Xiao YM, 2016. Optimization of shaking flask fermentation medium of Flammulina velutipes. China Brewing, 2016(11): 136-139 (in Chinese)

[22]	Zhong LJ, 2013. The Study on the biological characteristics of mycelia of wild Favolus arcularius. Edible Fungi, 35(5): 9-11 (in Chinese)

[23]	鲍大鹏, 汪滢, 高英女, 王莹, 李燕, 唐利华, 茅文俊, 周陈力, 龚明, 万佳宁, 杨瑞恒, 吴莹莹, 2019. 一种漏斗大孔菌免疫调节蛋白Fip-par1及其制备方法和应用技术. 中国:CN106496311B. 2019-07-05 本文引用 [1]

[24]	戴玉成, 2010. 中国东北地区木材腐朽菌的多样性. 菌物学报, 29: 801-818 本文引用 [1]

[25]	董浩然, 周峰, 李玉, 李正鹏, 姜宁, 2021. 营养液pH值对香菇菌丝生物量的影响. 上海蔬菜, 2021(4): 65-68 本文引用 [1]

[26]	段应策, 胡姿仪, 杨帆, 李金涛, 邬向丽, 张瑞颖, 2020. pH和缓冲作用对香菇菌丝生长的影响. 中国农业科学, 53(22): 4683-4690 本文引用 [1]

[27]	郭迪哲, 马敖, 胡佳君, 李丹, 张波, 李玉, 2019. 毛榆孔菌的固体培养、驯化栽培及其纤维素酶活性研究. 菌物学报, 38(7): 1111-1119 本文引用 [1]

[28]	黄年来, 1998. 中国大型真菌原色图鉴. 北京: 中国农业出版社. 59 本文引用 [1]

[29]	李玉, 2018. 中国食用菌产业发展现状:机遇和挑战--走中国特色菇业发展之路,实现食用菌产业强国之梦. 菌物研究, 16(3): 125-131 本文引用 [1]

[30]	刘娜, 张敏, 张士义, 李宏亮, 宋莹, 2020. 烟色离褶伞液体菌种培养条件优化及工厂化栽培研究. 中国食用菌, 39(10): 50-55 本文引用 [1]

[31]	刘伟, 蔡英丽, 何培新, 张亚, 边银丙, 2016. 梯棱羊肚菌无性孢子形态及显微结构观察. 菌物研究, 14(3): 157-161 本文引用 [1]

[32]	马璐, 杨驰, 肖冬来, 江晓凌, 应正河, 林衍铨, 2020. 碳氮源对广叶绣球菌液体发酵的影响. 中国食用菌, 39(10): 37-41+45 本文引用 [1]

[33]	祁亮亮, 安颖, 李玉, 2011. 褐褶边小奥德蘑生物学特性及驯化栽培. 食用菌学报, 18(3): 35-38 本文引用 [1]

[34]	王剑锋, 王璋, 李江, 饶军, 2009. 漏斗多孔菌液体发酵产漆酶条件研究. 菌物学报, 28(3): 440-444 本文引用 [1]

[35]	尹伟, 刘劲松, 吴培云, 梁益敏, 王刚, 2012. 漏斗大孔菌的化学成分研究. 天然产物研究与开发, 24(12): 1747-1749, 1756 本文引用 [1]

[36]	张伟彤, 姚方杰, 方明, 张友民, 马晓旭, 黄程远, 张越, 2021. 珊瑚猴头液体菌种及对栽培效果的研究. 东北农业科学, 2021(2): 125-131 本文引用 [1]

[37]	张怡, 王玉, 班立桐, 黄亮, 郑志广, 肖月梅, 2016. 白色金针菇摇瓶发酵培养基的优化研究. 中国酿造, 2016(11): 136-139 本文引用 [1]