宁夏白芨滩国家级自然保护区苦豆子内生真菌多样性及生态分布

doi:10.11733/j.issn.1007-0435.2016.06.023

草地学报 ›› 2016, Vol. 24 ›› Issue (6): 1309-1317.DOI: 10.11733/j.issn.1007-0435.2016.06.023

宁夏白芨滩国家级自然保护区苦豆子内生真菌多样性及生态分布

高媛^1,2, 周星辰¹, 孙牧笛¹, 吕苗苗¹, 顾沛雯¹

1. 宁夏大学农学院, 宁夏银川 750021;
2. 淮安出入境检验检疫局, 江苏淮安 223300

收稿日期:2015-07-07 修回日期:2016-07-15 出版日期:2016-12-15 发布日期:2017-03-18
通讯作者: 顾沛雯
作者简介:高媛(1988-),女,硕士研究生,主要从事生物防治与菌物资源利用,E-mail:elfish521@163.com
基金资助:
国家自然基金项目（31260452）资助

Diversity and Ecological Distribution of Endophytic Fungi of Sophora alopecuroides L. from Baijitan Nature Reserve of Ningxia

GAO Yuan^1,2, ZHOU Xing-chen¹, SUN Mu-di¹, LV Miao-miao¹, GU Pei-wen¹

1. Agricultural College, Ningxia University, Yinchuan, Ningxia Province 750021, China;
2. Huai'an entry-exit inspection and quarantine bureau, Huai'an, Jiangsu Province 223300, China

Received:2015-07-07 Revised:2016-07-15 Online:2016-12-15 Published:2017-03-18

摘要/Abstract

摘要：

为探索宁夏干旱荒漠区苦豆子（Sophora alopecuroides L.）内生真菌的多样性及区系组成，为苦豆子内生真菌的合理开发和利用提供理论依据。从宁夏白芨滩国家级自然保护区不同植被和土壤类型的6个样区采集苦豆子样品30份，采用组织匀浆法从植株的根、茎、叶和种子中分离内生真菌，根据培养性状、菌落、孢子等形态特征，结合rDNA-ITS序列分析对菌株进行鉴定；根据内生真菌的相对频率、物种多样性指数、丰富度指数和相似性系数分析其区系组成特点。结果表明，从30份样品中共分离得到内生真菌213株，分别属于19个属，其中镰刀菌属（Fusarium）、链格孢属（Alternaria）和茎点霉属（Phoma）为优势属。苦豆子内生真菌的分布具有一定的组织特异性，根部的内生真菌数量高于其他部位，茎部内生真菌多样性指数最高。植被类型中，沙生植被草原（样区Ⅴ）分离的内生真菌物种多样性指数最高，而荒漠草原（样区Ⅲ）最低。荒漠草原（样区Ⅰ）和沙生植被草原（样区Ⅴ）内生真菌群落有密切的相似性。可见，苦豆子蕴含丰富的内生真菌资源，其内生真菌具有很高的宿主特异性，且分布受生境影响。

关键词: 苦豆子, 内生真菌, 多样性, rDNA-ITS序列分析

Abstract:

The diversity and composition of endophytic fundi from Sophora alopecuroides L. in arid and desolate areas of Ningxia were analyzed, which could also provide research methods and theory on rational development and use of endophytic fungi from Sophora alopecuroides L.. The endophytic fungi were separated and cultivated from 30 samples of the healthy roots, stems, leaves and seeds of Sophora alopecuroides L. by tissue-homogenate in six sample plots, where were sampled from different vegetations and soil types in the Baijitan National Nature Reserve of Ningxia. Endophytic fungi were classified according to their morphological characteristics of culture, bacterial colonies and spores and rDNA-ITS sequences analysis. Relative frequency (Rf), Shannon-wiener index (H), Margalef index (D) and Sorenson's similarity coefficient (CS) were calculated to analyze their characteristics of composition. The results showed that, 213 endophytic fungi were isolated from 30 samples, and they belong to 19 different genera, in which Fusarium, Alternaria and Phoma were dominant genera. The distribution of endophytic fungi from Sophora alopecuroides L. has some degree of tissue preference. Quantities of endophytic fungi from roots of Sophora alopecuroides L. were more than other tissues for the same plant. Index of diversity of endophytic fungi from stem of Sophora alopecuroides L. was the highest. However some similarity does exist for fungi found in Sophora alopecuroides L. under different living habitat. Shannon-wiener index (H) of endophytic fungi from Sophora alopecuroides L. were the most significantly in the sandy weeds(sample Ⅴ) and the least in desert steppes(sample Ⅲ). The community of endophytic fungi from Sophora alopecuroides L. of desert steppe(sampleⅠ) and sandy weeds(sample Ⅴ) have some similarity. Our results showed that the Sophora alopecuroides L. has abundant resources of endophytic fungi and the species diversity of endophytic fungi communities from Sophora alopecuroides L. is affected both by its host preference and by its different habitats.

Key words: Sophora alopecuroides L., Endophytic fungi, Diversity, rDNA-ITS sequence analysis

中图分类号:

高媛, 周星辰, 孙牧笛, 吕苗苗, 顾沛雯. 宁夏白芨滩国家级自然保护区苦豆子内生真菌多样性及生态分布[J]. 草地学报, 2016, 24(6): 1309-1317.

GAO Yuan, ZHOU Xing-chen, SUN Mu-di, LV Miao-miao, GU Pei-wen. Diversity and Ecological Distribution of Endophytic Fungi of Sophora alopecuroides L. from Baijitan Nature Reserve of Ningxia[J]. Acta Agrestia Sinica, 2016, 24(6): 1309-1317.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2016.06.023

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2016/V24/I6/1309

参考文献

[1] 郭良栋. 内生真菌研究进展[J]. 菌物系统,2001,20(1):148-152
[2] 厉秀秀,方玉鹏,赖毕,等. 黄连产小檗碱内生真菌的分离鉴定[J]. 草地学报,2013,21(5):1005-1011
[3] 李莉,张文学,张顺利. 苦豆子生物碱的免疫调节作用及其作用机制研究进展[J]. 中草药,2007,38(4):7-8
[4] 孙剑秋,郭良栋,臧威,等. 药用植物内生真菌多样性及生态分布[J]. 中国科学C辑:生命科学,2008,38(5):475-484
[5] Kumar D S, Hyde K D. Biodiversity and tissue-specificity of endophytic fungi in Tripterygium wilfordii[J]. Fungal Diversity,2004,17:69-90
[6] Porras-Alfaro A, Bayman P. Hidden fungi,emergent properties:Endophytes and microbiomes[J]. Annual Review of Phytopathology,2001,49:291-315
[7] Ghimire S R, Charlton N D, et al. Biodiversity of fungal endophyte communities inhabiting switchgrass (Panicum virgatum L.)growing in the native tallgrass prairie of northern Oklahoma[J].Fungal Diversity,2010,47(1):19-27
[8] 臧威,孙翔,孙剑秋. 南方红豆杉内生真菌的多样性与群落结构[J]. 应用生态学报,2014,7(25):2071-2078
[9] 张少鹏,胥婷,杨丽强,等. 不同草原类型针茅根部内生真菌群落结构[J]. 应用生态学报,2014,25(12):3475-3482
[10] Lu H, Zou W X, Meng J C. New bioactive metabolites produced by Colletotrichum sp.,an endophytic fungus in Artemisia annua[J]. Plant Science,2000,151:67-73
[11] Clay K, Cheplick G P, Wray S M. Impact of the fungus Balansin henningsiana on the grass Panicum agrostoides:frequency of infection,plant growth and reproduction and resistance to pests[J]. Oecologia,1989,80:374-380
[12] Funk C R, White R H, Breen J P. Importance of Acremonium endophytes in turf-grass breeding and management[J]. Agriculture Ecosystems and Environment,1993,44:215-232
[13] 梁宇,高玉葆. 内生真菌对植物生长发育及抗逆性的影响[J]. 植物学通报,2000,17(1):52-59
[14] Rice J S, Pinkerton B W, Stinger W C, et al. Seed production in tall fescue as affected by fungal endophyets[J]. Crop Science,1990,30:1303-1305
[15] 周福生,穆青. 野生植物苦豆子的化学成分和主要药理作用[J]. 中国野生植物资源,2006,25(4):1-3.
[16] Kant S, Kant P, Raveh E, et al. Evidence that differential gene expression between the halophyte, Thellungiella halophila, and Arabidopsis thaliana is responsible for higher levels of the compatible osmolyte proline and tight control of Na⁺uptake in T. halophila[J]. Plant,Cell and Environment,2006,29(7):1220-1234
[17] 李艳艳,冯俊涛,张兴,等. 苦豆子化学成分及其生物活性研究进展[J]. 西北农业学报,2005,14(2):133-136
[18] 顾沛雯,郝丽,徐润,等. 宁夏白芨滩自然保护区苦豆子内生真菌的区系组成及其抑菌活性[J]. 西北农林科技大学学报(自然科学版),2012,40(5):209-216
[19] 刘建利,林勤,闫兴富. 宁夏地区苦豆子内生真菌的分离与初步鉴定[J]. 干旱地区农业研究,2012,30(5):193-199
[20] 周星辰,郝丽,张伟,等. 产喹诺里西啶碱苦豆子内生真菌的筛选与鉴定[J]. 农业科研究,2013,27(1):28-32
[21] 张延威. 石斛内生真菌及其分子系统学研究[D]. 贵阳:贵州大学,2005
[22] Stackebrandt E, Goebel B M. Taxonomicnote:aplace for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology[J]. International Journal of Systematic Bacteriology,1994,44:846-849
[23] 高晓原,贝盏临,雷茜,等. 宁夏苦豆子资源基本情况及综合开发现状[J]. 中国野生植物资源,2009,28(2):17-20
[24] 马瑜,王莉衡,李英梅,等. 库拉索芦荟内生真菌菌群的多样性研究[J]. 微生物学通报,2014,41(11):2235-2243
[25] Aly A H,Debbab A,Proksch P. Fungal endophytes:Unique plant inhabitants with great promises[J]. Applied Microbiology and Biotechnology,2011,90:1829-1845
[26] 曹丹丹,路浩,李小蒙,等. 茎直黄芪内生真菌分离鉴定及多样性分析[J]. 草地学报,2015,23(6):1252-1258
[27] 毕江涛,潘润霞,李乐,等. 牛心朴子内生真菌分离及其抑菌活性分析[J]. 草地学报,2014,22(1):188-193
[28] Marilize L R,Paul R M. Nonomuraea candida sp. nov., a new species from South African soil[J]. Antonie van Leeuwenhoek,2008(93):133-139
[29] 黄晓辉,李珊,谭周进,等. 植物内生放线菌研究进展[J]. 生物技术通报,2008,1:42-46
[30] 姚领爱,胡之璧,王莉莉,等. 植物内生菌与宿主关系研究进展[J]. 生态环境学报,2010,19(7):1750-1754

宁夏白芨滩国家级自然保护区苦豆子内生真菌多样性及生态分布

Diversity and Ecological Distribution of Endophytic Fungi of Sophora alopecuroides L. from Baijitan Nature Reserve of Ningxia

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	姚世庭, 芦光新, 邓晔, 范月君, 周华坤, 党宁, 王英成, 张海娟, 颜珲璘. 模拟增温对高寒草地土壤原核生物群落组成及多样性影响[J]. 草地学报, 2021, 29(S1): 27-34.
[2]	佘延娣, 杨晓渊, 马丽, 张中华, 王党军, 黄小涛, 马真, 姚步青, 周华坤. 退化高寒草甸植物群落和土壤特征及其相互关系研究[J]. 草地学报, 2021, 29(S1): 62-71.
[3]	吕婷, 刘玉萍, 亢俊铧, 刘涛, 梁瑞芳, 苏旭. 德令哈-哈拉湖沿线不同生境下群落的物种组成及其多样性[J]. 草地学报, 2021, 29(S1): 146-155.
[4]	晏和飘, 李文龙, 梁天刚, 周华坤, 曹亚鹏, 廖元成. 青藏高原退化高寒草地恢复对不同措施响应的Meta分析[J]. 草地学报, 2021, 29(S1): 190-198.
[5]	张中华, 马丽, 周秉荣, 宋明华, 徐文华, 邓艳芳, 王芳, 佘延娣, 张骞, 姚步青, 马真, 周华坤. 高寒草甸优势种功能多样性对增温和模拟放牧的响应[J]. 草地学报, 2021, 29(S1): 225-232.
[6]	马骢毓, 韩重阳, 马赛男, 李旭旭, 蔡家邦, 汪阳, 张新全, 聂刚. 基于SSR分子标记的13个白三叶(Trifolium repens L.)品种指纹图谱构建[J]. 草地学报, 2021, 29(9): 1892-1899.
[7]	王占青, 张杰雪, 杨雪莲, 黄霞, 陈斯亮, 乔有明. 高寒草甸不同斑块草地土壤微生物多样性特征研究[J]. 草地学报, 2021, 29(9): 1916-1926.
[8]	罗铭欣, 刘凤民, 陈纪言, 崔均涛, 张伟丽. ⁶⁰Coγ辐射柱花草M₃代的农艺性状及遗传多样性分析[J]. 草地学报, 2021, 29(9): 1992-2000.
[9]	梁小玉, 季杨, 胡远彬, 易军, 白史且, 张靓, 张新全. 菊苣EST-SSR分子标记开发及通用性分析[J]. 草地学报, 2021, 29(9): 2081-2090.
[10]	连鹤娜, 陈泰祥, 陈振江, 魏学凯, 李春杰. 内生真菌醉马草作为机场驱鸟草的潜力分析[J]. 草地学报, 2021, 29(8): 1649-1657.
[11]	唐丽辉, 刘奕伶, 赵文星, 张云昊, 吴可欣, 郭梓豫, 莫重辉, 赵宝玉, 路浩. 箭叶橐吾内生真菌分离鉴定及组织分布特性研究[J]. 草地学报, 2021, 29(8): 1658-1665.
[12]	孙宇, 张峰, 郑佳华, 赵天启, 赵萌莉, 张彬. 刈割留茬高度对大针茅草原物种多样性及地上生物量的影响[J]. 草地学报, 2021, 29(8): 1859-1864.
[13]	刘博文, 黎桂阳, 常媛飞, 金美燕, 刘芳, 高秋, 刘万良, 哈斯塔米尔, 王显国. 野豌豆属种子形态多样性与种子分类鉴定方法的研究[J]. 草地学报, 2021, 29(7): 1375-1385.
[14]	刘凌云, 范希峰, 滕珂, 岳跃森, 常智慧, 武菊英. 苔草属植物遗传多样性研究进展[J]. 草地学报, 2021, 29(6): 1148-1157.
[15]	刘玉祯, 刘文亭, 冯斌, 时光, 孙彩彩, 李彩弟, 张小芳, 董全民. 坡向和海拔对高寒山地草甸植被分布格局特征的影响[J]. 草地学报, 2021, 29(6): 1166-1173.