外源硫化氢对不同弱光下高羊茅形态和生理指标的影响

doi:10.11733/j.issn.1007-0435.2021.11.008

草地学报 ›› 2021, Vol. 29 ›› Issue (11): 2435-2441.DOI: 10.11733/j.issn.1007-0435.2021.11.008

外源硫化氢对不同弱光下高羊茅形态和生理指标的影响

李萱, 刘博文, 高双红, 游翔凯, 龙思, 李尤月, 刘茜, 张士军, 许岳飞

西北农林科技大学草业与草原学院, 陕西杨凌 712100

收稿日期:2021-05-09 修回日期:2021-07-05 出版日期:2021-11-15 发布日期:2021-12-06
通讯作者: 许岳飞,E-mail:xuyuefei@nwsuaf.edu.cn
作者简介:李萱(2000-),女,汉族,甘肃兰州人,在读本科生,主要从事牧草、草坪草逆境生物学研究,E-mail:2514392577@qq.com
基金资助:
国家自然科学基金项目（32071888）；陕西省重点研发计划-一般项目（2021NY-003）；中央高校基本科研业务费专项资金资助（2452019080）；陕西省重点研发计划-重点产业链（No.2021ZDLNY02-04；No.2020ZDLNY02-05）资助

Effects of Exogenous Hydrogen Sulfide on Morphology and Physiological Indexes of Tall Fescue under Low Light Stress

LI Xuan, LIU Bo-wen, GAO Shuang-hong, YOU Xiang-kai, LONG Si, LI You-yue, LIU Qian, ZHANG Shi-jun, XU Yue-fei

College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China

Received:2021-05-09 Revised:2021-07-05 Online:2021-11-15 Published:2021-12-06

摘要/Abstract

摘要： 本研究以冷季型草坪草高羊茅‘Arid3’（Festuca arundinacea Schreb.‘Arid3’）为研究材料，采用室内模拟弱光胁迫，研究喷施外源硫化氢（H₂S）供体硫氢化钠对不同光照强度（100，75，50和25 μmol·m^-2·s^-1）处理下高羊茅形态指标、光合色素、细胞膜透性和内源激素的影响，旨在为耐荫草坪草分子育种提供理论基础。研究结果显示：随着光照强度的降低，高羊茅形态指标和生理指标都不同程度的降低，而喷施外源H₂S供体能够显著提高光合色素含量，降低相对电导率和丙二醛含量（P<0.05），且在光照强度为50 μmol·m^-2·s^-1和75 μmol·m^-2·s^-1时效果较为显著。同时，喷施外源H₂S供体能够促进适度弱光下高羊茅内源脱落酸（Abscisic acid，ABA）、赤霉素（Gibberellin，GA）和硫化氢含量的增加（P<0.05）。综上，适度的弱光下，外源H₂S通过提高高羊茅内源激素含量，保护细胞膜完整性，增加光合色素含量，提高高羊茅的耐荫性。

关键词: 硫化氢, 高羊茅, 弱光胁迫, 细胞膜稳定性, 内源激素

Abstract: This study was conducted to determine the effects of sodium hydrosulfide which is the donor of exogenous hydrogen sulfide (H₂S) on the morphological indicators,photosynthetic pigment,membrane permeability and endogenous hormones of tall fescue (‘Arid3’) under different kinds of light intensity (100,75,50 and 25 μmol·m^-2·s^-1),in order to provide theoretical basis for molecular breeding of shade-tolerant turfgrass. The results showed that the morphological and physiological indexes of tall fescue decreased with different degrees with the decrease of light intensity. Spraying exogenous H₂S donor could significantly increase the content of photosynthetic pigment,reduce the relative conductivity and MDA content (P<0.05),especially when light intensity was 50 and 75 μmol·m^-2·s^-1. Meanwhile,the exogenous H₂S donor could promote the content of abscisic acid (ABA),gibberellin (GA) and hydrogen sulfide under suitable low light. In conclusion,the application of exogenous H₂S can improve the shade tolerance of tall fescue by protecting the integrity of cell membrane,increasing photosynthetic pigment and endogenous hormone content under suitable low light stress.

Key words: Hydrogen sulfide, Tall fescue, Low light stress, Cell membrane stability, Endogenous hormone

中图分类号:

Q945.78

李萱, 刘博文, 高双红, 游翔凯, 龙思, 李尤月, 刘茜, 张士军, 许岳飞. 外源硫化氢对不同弱光下高羊茅形态和生理指标的影响[J]. 草地学报, 2021, 29(11): 2435-2441.

LI Xuan, LIU Bo-wen, GAO Shuang-hong, YOU Xiang-kai, LONG Si, LI You-yue, LIU Qian, ZHANG Shi-jun, XU Yue-fei. Effects of Exogenous Hydrogen Sulfide on Morphology and Physiological Indexes of Tall Fescue under Low Light Stress[J]. Acta Agrestia Sinica, 2021, 29(11): 2435-2441.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2021.11.008

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2021/V29/I11/2435

参考文献

[1] 罗艺岚,赵东豪,孙鹏越,等. 遮阴胁迫对麦冬和多年生黑麦草根际土壤细菌群落结构和多样性的影响[J]. 草地学报,2019,27(5):1204-1212
[2] 许岳飞,金晶炜,陈浩,等. 草坪植物耐弱光机理研究进展[J]. 草地学报,2011,19(6):1064-1069
[3] 尹慧,安莹,陈雅君,等. 不同遮阴强度下白三叶形态特征和生长动态[J]. 中国草地学报,2015,37(5):86-91
[4] KABIL O,VITVITSKY V,BANERJEE R. Sulfur as a signaling nutrient through hydrogen sulfide[J]. Annual Review of Nutrition,2014,34:171-205
[5] GOTOR C,GARCÍA I,AROCA Á,et al. Signaling by hydrogen sulfide and cyanide through post-translational modification[J]. Journal of Experimental Botany,2019,70(6):4251-4265
[6] ZHANG H,HU L Y,HU K D,et al. Hydrogen sulfide promotes wheat seed germination and alleviates oxidative damage against copper stress[J]. Journal of Integrative Plant Biology,2008,50(12):1518-1529
[7] ZHOU Z H,WANG Y,YE X Y,et al. Signaling molecule hydrogen sulfide improves seed germination and seedling growth of maize (Zea mays L.) under high temperature by inducing antioxidant system and osmolyte biosynthesis[J]. Frontiers in Plant Science,2018,9:1288
[8] FANG L C,JU W L,YANG C L,et al. Application of signaling molecules in reducing metal accumulation in alfalfa and alleviating metal-induced phytotoxicity in Pb/Cd-contaminated soil[J]. Ecotoxicology and Environmental Safety,2019,182:109459
[9] WANG Y Q,LI L,CUI W T,et al. Hydrogen sulfide enhances alfalfa (Medicago sativa) tolerance against salinity during seed germination by nitric oxide pathway[J]. Plant and Soil,2012,351(1-2):107-119
[10] CHRYSTALLA A,RAFAELLA X,GIANNIS C,et al. Exploring the potential of nitric oxide and hydrogen sulfide (NOSH)-releasing synthetic compounds as novel priming agents against drought stress in Medicago sativa plants[J]. Biomolecules,2020,10(1):120
[11] 刘茜,刘博文,王洋,等. UV-B辐射对不同生态型垂穗披碱草幼苗生长和抗氧化酶系统的影响[J]. 草地学报,2019,27(4):898-905
[12] 赵丽丽,吴佳海,陈莹,等. 干旱对高羊茅生理特性的影响及抗旱性评价[J]. 中国草地学报,2015,37(4):15-20
[13] 杨志民,陈煜,韩烈保,等. 不同光照强度对高羊茅形态和生理指标的影响[J]. 草业学报,2007,16(6):23-29
[14] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000:134-261
[15] 刘霄霏,李惠英,陈良,等. 外源硒对镉胁迫下黑麦草生长和生理的影响[J]. 草地学报,2020,28(1):72-79
[16] 吴颂如,陈婉芬,周燮. 酶联免疫法(ELISA)测定内源植物激素[J]. 植物生理学通讯,1988(5):53-57
[17] JIANG J L,TIAN Y,LI L,et al. H₂S alleviates salinity stress in cucumber by maintaining the Na⁺/K⁺ balance and regulating H₂S metabolism and oxidative stress response[J]. Frontiers in Plant Science,2019,10:678
[18] 付娟娟,张瀚文,陈浩,等. 不同遮荫方式对高羊茅形态和生理变化的影响[J]. 北方园艺,2012(7):46-48
[19] KOH K J,BELL G E,MARTIN D L,et al. Shade and airflow restriction effects on creeping bentgrass golf greens[J]. Crop Science,2003,43(6):2182-2188
[20] 秦衍雷,武菊英,徐迎春,等. 遮荫对观赏草丽色画眉生长、光合以及生物量的影响[J]. 草地学报,2013,21(5):921-927
[21] 韩忠明,赵淑杰,刘翠晶,等. 遮荫对3年生东北铁线莲生长特性及品质的影响[J]. 生态学报,2011,31(20):6005-6012
[22] 李云飞. 不同遮荫度对鼠尾草生长的影响[J]. 北方园艺,2009(9):168-169
[23] 孙小玲,许岳飞,马鲁沂,等. 植株叶片的光合色素构成对遮阴的响应[J]. 植物生态学报,2010,34(8):989-999
[24] 战丽杰,张宏宝,李宗泰,等. 遮荫处理对芍药幼苗生长和矿质营养积累的影响[J]. 应用生态学报,2020,31(10):3473-3479
[25] 熊雪,周禾,高伟平. 遮荫胁迫下外源一氧化氮对高羊茅的生理调控作用[J]. 草地学报,2012,20(2):331-335
[26] YANG A J,ANJUM S A,WANG L,et al. Effect of foliar application of brassinolide on photosynthesis and chlorophyll fluorescence traits of leymus chinensis under varying levels of shade[J]. Photosynthetica,2018,56(3):873-883
[27] 陈卫东,张玉霞,丛百明,等. 钾肥对紫花苜蓿根颈丙二醛、可溶性蛋白含量与抗氧化系统的影响[J]. 草地学报,2021,29(4):717-723
[28] HOFFMANN-BENNING S,KENDE H. On the role of abscisic acid and gibberellin in the regulation of growth in rice[J]. Plant Physiology,1992,99(3):1156-1161
[29] HE H Y,CARLOS G M,HE L F. Interaction between hydrogen sulfide and hormones in plant physiological responses[J]. Plant Growth Regulation,2019,87(1):175-186
[30] 丘立杭,李强,黄杏,等. 弱光胁迫影响甘蔗叶片内源激素的平衡和分蘖进程[J]. 植物生理学报,2017,53(2):280-290
[31] 韩晨静,王磊,王琦,等. 遮阴对油用牡丹叶片衰老生理指标的影响[J]. 核农学报,2019,33(12):2492-2498
[32] 王韬远,陈长征,陶冶,等. 硫化氢与植物激素脱落酸对拟南芥气孔运动的调节作用[J]. 分子植物育种,2018,16(23):7834-7838
[33] KAYA C,SARIOǦLU A,ASHRAF M,et al. Gibberellic acid-induced generation of hydrogen sulfide alleviates boron toxicity in tomato (Solanum lycopersicum L.) plants[J]. Plant Physiology and Biochemistry,2020,153:53-63
[34] LI Z G,YANG S Z,LONG W B,et al. Hydrogen sulphide may be a novel downstream signal molecule in nitric oxide-induced heat tolerance of maize (Zea mays L.) seedlings[J]. Plant Cell Environment,2013,36(8):1564-1572
[35] 陈明媛,张淑英,王梦柯,等. 外源硫化氢对NaCl胁迫下棉花幼苗抗逆生理特性的影响[J]. 干旱地区农业研究,2021(2):55-61
[36] 朱婷,章陆杨,许志敏,等. 遮光及复光处理对紫叶狼尾草叶色及生理变化的影响[J]. 草地学报,2019,27(1):263-268
[37] 王红燕,张淑英. 外源硫化氢对盐胁迫下棉花幼苗生长及渗透调节系统的影响[J]. 西南农业学报,2020,33(11):2483-2489

外源硫化氢对不同弱光下高羊茅形态和生理指标的影响

Effects of Exogenous Hydrogen Sulfide on Morphology and Physiological Indexes of Tall Fescue under Low Light Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	高双红, 李媛英, 刘博文, 龙思, 刘铁芫, 许岳飞. H₂S对弱光胁迫下高羊茅幼苗生理特性和内源激素的影响[J]. 草地学报, 2021, 29(10): 2233-2239.
[2]	马雪瑶, 隋晓青, 李俊明, 张博. 叶面喷施GA₃对紫花苜蓿花荚内源激素及种子产量构成的影响[J]. 草地学报, 2020, 28(5): 1294-1300.
[3]	杜利霞, 赵凯玥, 王凯鑫, 刘伟, 钟华, 夏方山, 朱慧森. 6-BA引发对赖草种子萌发、贮藏物质和内源激素的影响[J]. 草地学报, 2020, 28(3): 667-674.
[4]	刘春阳, 李占台, 刘东霞, 王激清, 刘贵河. 速效氮肥和缓释肥配施对高羊茅草坪质量及土壤硝态氮动态的影响[J]. 草地学报, 2018, 26(5): 1248-1254.
[5]	宋佳琦, 王玉祥, 张博. 内源激素变化及外源生长素对紫花苜蓿种子萌发过程的影响[J]. 草地学报, 2018, 26(3): 691-696.
[6]	李昱彤, 齐实, 周金星, 郝文乾, 靳孟理, 胡译水. 城市废弃物堆肥的混配基质对高羊茅营养生长期的影响[J]. 草地学报, 2017, 25(5): 1069-1078.
[7]	马艳芳, 张旭虎, 孟雯雯, 柴睿, 祁彦文, 许岳飞. 外源一氧化氮及不同修剪高度对高羊茅根形态特征的影响[J]. 草地学报, 2017, 25(4): 777-781.
[8]	王宝媛, 濮阳雪华, 宋桂龙, 周述琼, 赵克奇, 蒋凯. 20个高羊茅品种镉耐性评价及富集特征[J]. 草地学报, 2017, 25(1): 107-114.
[9]	张咏梅, 马晖玲, 张锁科, 唐云智. 高效液相色谱法测定草地早熟禾4种内源激素的方法[J]. 草地学报, 2016, 24(6): 1384-1387.
[10]	李尚玮, 杨文权, 赵冉, 韩明玉, 寇建村. 果树行间生草对苹果园土壤肥力的影响[J]. 草地学报, 2016, 24(4): 895-900.
[11]	孙鏖, 邓荟芬, 刘海林, 易康乐, 李剑波, 伍佰鑫, 李昊帮, 蒋桂韬, 戴求仲. 干旱胁迫对苎麻苗期理化指标的影响[J]. 草地学报, 2016, 24(3): 577-580.
[12]	赵雁, 马向丽, 车伟光, 毕玉芬. 高温胁迫下‘德钦’紫花苜蓿内源激素的变化[J]. 草地学报, 2016, 24(2): 358-362.
[13]	王晓英. 刺柏叶水提液对高羊茅的化感作用及其GC-MS分析[J]. 草地学报, 2016, 24(2): 363-368.
[14]	孙鑫博, 韩烈保. 亚精胺、精胺对结缕草低温下内源激素含量及内源多胺代谢的影响[J]. 草地学报, 2015, 23(4): 804-810.
[15]	赵丽丽, 吴佳海, 王普昶, 马林, 杨春燕, 卢立娜. 高羊茅种质光合及叶绿素荧光参数对高温胁迫的响应[J]. 草地学报, 2015, 23(4): 811-817.