外源茉莉酸甲酯对镉胁迫下高羊茅生长及生理指标的影响

doi:10.11733/j.issn.1007-0435.2022.04.024

草地学报 ›› 2022, Vol. 30 ›› Issue (4): 974-982.DOI: 10.11733/j.issn.1007-0435.2022.04.024

• 研究论文 • 上一篇

外源茉莉酸甲酯对镉胁迫下高羊茅生长及生理指标的影响

马志强, 史毅, 种培芳

甘肃农业大学林学院, 甘肃兰州 730070

收稿日期:2021-10-15 修回日期:2021-11-08 发布日期:2022-04-25
通讯作者: 种培芳,E-mail:zhongpf@gsau.edu.cn
作者简介:马志强(1996-),男,回族,甘肃兰州人,硕士研究生,主要从事治理重金属污染的研究,E-mail:1107579833@qq.com
基金资助:
丝绸之路"草原生态修复技术模式研究与示范,对发展中国家常规性科技援助项目(KY202002011);甘肃省自然科学基金(20 JR5RA035);甘肃农业大学学科建设基金——植物根际益生菌挥发性化合物类似物诱导匍匐翦股颖耐热性机理研究(GAU-XKJS-2018-107)资助

Effects of Exogenous Methyl Jasmonate on Growth and Physiological Indexes of Festuca elata under Cadmium Stress

MA Zhi-qiang, SHI Yi, ZHONG Pei-fang

College of forestry, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China

Received:2021-10-15 Revised:2021-11-08 Published:2022-04-25

摘要/Abstract

摘要： 本研究以镉污染土壤为研究对象,茉莉酸甲酯(MeJA)为缓解剂,将高羊茅作为镉污染土壤的修复草种,通过测定高羊茅的相关生理指标、以及植物和土壤中的镉含量,探究MeJA对高羊茅修复镉污染土壤的影响。结果表明,较单一Cd胁迫(100 μmol·L^-1),施用MeJA (25~75 μmol·L^-1)可使高羊茅的叶绿素含量、抗氧化酶活性升高,丙二醛(MDA)、游离脯氨酸(Pro)、过氧化氢(H₂O₂)和超氧自由基(·O^-₂)含量下降;施用<75 μmol·L^-1的MeJA可使高羊茅根系和叶片的镉含量最高上升59.58%和38.64%,且土壤的镉含量最多下降27.49%;50 μmol·L^-1的MeJA浓度下,高羊茅根系鲜重及长度增加了136.79%和58.18%,超过此浓度后,高羊茅代谢被影响,植物体对镉吸附能力减弱。说明一定浓度的MeJA可增强高羊茅的生理活性,从而减轻Cd²⁺对高羊茅的损害,进一步加强高羊茅对镉污染土壤的修复力。

关键词: 镉胁迫, 茉莉酸甲酯, 高羊茅, 幼苗生理特性, 重金属修复

Abstract: The impact of heavy metals on the environment has endangered the safety of human survival.How to effectively repair heavy met al damage to the environment has been one of the hot spots.In this study,cadmium-contaminated soil was used as the research object,methyl jasmonate was used as a mitigation agent,and Festuca elata was used as a remediation grass species of cadmium-contaminated soil.The effects of MeJA on the remediation of cadmium-contaminated soil by Festuca elata were explored by measuring the related physiological indexes of Festuca elata,cadmium content in plants and soil.The results showed that compared with single Cd (100 μ mol·L^-1) stress,(1) MeJA (25~75 μ mol·L^-1) increased the chlorophyll content and antioxidant enzyme activity of Festuca elata,and decreased the contents of malondialdehyde (MDA),free proline (Pro),hydrogen peroxide (H₂O₂) and superoxide radical (·O^-₂).(2) The application of MeJA at the concentration of <75 μ mol·L^-1 increased the cadmium content in roots and leaves of Festuca elataby 59.58% and 38.64%,while the cadmium content in soil decreased by 27.49%.(3) The fresh weight and length of Festuca elata root increased by 136.79% and 58.18% under 50 μ mol·L^-1 MeJA concentration.When MeJA exceeded this concentration,the metabolism of Festuca elata was affected,and the adsorption capacity of plants to cadmium was weakened.The results showed that MeJA could enhance the physiological activity of Festuca elata,reduce the damage of Cd²⁺ to Festuca elata,and further strengthen the remediation ability of Festuca elata to cadmium contaminated soil.

Key words: Cadmium stress, Methyl jasmonate, Festuca elata, Physiological characteristics of seedlings, Heavy metal remediation

中图分类号:

S153

马志强, 史毅, 种培芳. 外源茉莉酸甲酯对镉胁迫下高羊茅生长及生理指标的影响[J]. 草地学报, 2022, 30(4): 974-982.

MA Zhi-qiang, SHI Yi, ZHONG Pei-fang. Effects of Exogenous Methyl Jasmonate on Growth and Physiological Indexes of Festuca elata under Cadmium Stress[J]. Acta Agrestia Sinica, 2022, 30(4): 974-982.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2022.04.024

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2022/V30/I4/974

参考文献

[1] 骆永明,滕应.我国土壤污染的区域差异与分区治理修复策略[J].中国科学院院刊,2018,33(02):145-152
[2] 国土资源部.环境保护部和国土资源部发布全国土壤污染状况调查公报[J].资源与人居环境,2014(04):26-27
[3] 国家环境保护局,中国环境监测总站.中国土壤元素背景值[M].北京:中国环境科学出版社,1990:87-90
[4] 陈能场,郑煜基,何晓峰,等.《全国土壤污染状况调查公报》探析[J].农业环境科学学报,2017,36(9):1689-1692
[5] LEI L,MA Y,ZHANG S,et al. An inventory of trace element inputs to agricultural soils in China[J]. Journal of Environmental Management,2009,90(8):2524-2530
[6] 苏国臣.沈阳市环境污染对人体健康影响的现状与展望[J].中国环境科学,1987,7(5):76-78
[7] 陈涛,吴燕玉,孔庆新,等.含镉工业污水对农田污染的防治和土地利用——生态农业工程初探[J].环境科学学报,1986,6(4):379-384
[8] 张星雨,叶志彪,张余洋.植物响应镉胁迫的生理与分子机制研究进展[J].植物生理学报,2021,57(07):1437-1450
[9] 铁梅,张朝红,董厚德,等.草坪草对土壤中镉的吸收与富集作用的研究[J].辽宁大学学报(自然科学版),2001,28(002):189-190
[10] LUO H,LI H,ZHANG X,et al. Antioxidant responses and gene expression in perennial ryegrass (Lolium perenne L.) under cadmium stress[J]. Ecotoxicology,2011,20(4):770-8
[11] 杨卓. Cd、Pb、Zn污染潮褐土的植物修复及其强化技术研究[D].保定:河北农业大学,2009:33-35
[12] 韩烈保,杨碚,邓菊芬.草坪草种及其品种[M].北京:中国林业出版社,1999:89-95
[13] 郑轶琦,臧国长,王青风.聚乙二醇胁迫对高羊茅种子萌发和幼苗生长的影响[J].北方园艺,2012(2):70-72
[14] 赵丽丽,吴佳海,王普昶,等.高羊茅种质光合及叶绿素荧光参数对高温胁迫的响应[J].草地学报,2015,23(04):811-817
[15] 王晓桐,柳玉霞,苏旭东,等.高羊茅对重金属镉的抗性分析[J].中国农学通报,2012,28(04):192-199
[16] 徐佩贤,费凌,陈旭兵,等.四种冷季型草坪植物对镉的耐受性与积累特性[J].草业学报,2014,23(06):176-188
[17] 王宝媛,濮阳雪华,宋桂龙,等. 20个高羊茅品种镉耐性评价及富集特征[J].草地学报,2017,25(01):107-114
[18] 王新,周启星,陈涛,等.污泥土地利用对草坪草及土壤的影响[J].环境科学,2003,24(2):50-53
[19] 张金彪,黄维南.镉对植物的生理生态效应的研究进展[J].生态学报,2000,20(3):514-523
[20] 韩梦梦,宋桂龙,隋永超. DMTU对镉胁迫下高羊茅根系的缓解作用[J].草业科学,2020,37(08):1488-1496
[21] 王舒婷.柠檬酸和EDTA对高羊茅和草地早熟禾体内Cd富集的调控[D].上海:上海交通大学,2018:3
[22] 薛仁镐,金圣爱.茉莉酸甲酯:一种重要的植物信号转导分子[J].生物技术通讯,2006,17(6):985-988
[23] SWIATEK A,DONGEN W V,ESMANS E L,et al. Metabolic Fate of Jasmonates in Tobacco Bright Yellow-2 Cells[J]. Plant physiology,2004,135(1):161-172
[24] 陈俊.外源茉莉酸酮酯对红树幼苗重金属抗性的调控作用研究[D].上海:华东师范大学,2014:83-85
[25] 鲜靖苹,王勇,张平.外源茉莉酸甲酯对镉胁迫下多年生黑麦草种子萌发及幼苗生理的影响[J].西北师范大学学报(自然科学版),2019,55(06):74-82
[26] 刘宇婧,冯艺玫,刘欣悦,等.镉对波斯菊种子发芽的影响及外源MeJA的缓解作用[J].草业学报,2017,26(01):122-130
[27] 徐邦发,徐雅丽.棉花叶片的叶绿素含量测定[J].塔里木农垦大学学报,1995,7(2):29-32
[28] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:105-261
[29] BEERS R F,SIZER I W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase[J]. Journal of Biological Chemistry,1952,195(1):133-133
[30] 黄美玉.高羊茅对重金属镉的抗性机理研究[D].武汉:中南民族大学,2019:14-15
[31] 马建宏,李阳.石墨炉原子吸收光谱法测定土壤中重金属铅、镉的方法改进[J].理化检验(化学分册),2020,56(01):94-96
[32] 段瑞军,吴朝波,王蕾,等.镉胁迫对海雀稗脯氨酸、可溶性糖和叶绿素含量及氮、磷、钾吸收的影响[J].江苏农业学报,2016,32(02):357-361
[33] ASSCHE F V,CLIJSTERS H. Effects of met als on enzyme activity in plants[J]. Plant Cell&Environment,2010,13(3):195-206
[34] PER T S,KHAN M,ANJUM N A,et al. Jasmonates in plants under abiotic stresses:Crosstalk with other phytohormones matters[J]. Environmental&Experimental Botany,2018,145(2):104-120
[35] E SÁNCHEZ-RODRÍGUEZ,RUBIO-WILHELMI M,BLASCO B,et al. Antioxidant response resides in the shoot in reciprocal grafts of drought-tolerant and drought-sensitive cultivars in tomato under water stress[J]. Plant Science,2012,188-189(none):89-96
[36] HU L,LI H,PANG H,et al. Responses of antioxidant gene,protein and enzymes to salinity stress in two genotypes of perennial ryegrass (Lolium perenne) differing in salt tolerance[J]. Journal of Plant Physiology,2012,169(2):146-156
[37] MITTLER R. Oxidative stress,antioxidants and stress tolerance[J]. Trends in Plant Science,2002,7(9):405-410
[38] KUBILAY G,GüL?AH K,MUSTAFA,et al. Development of a fluorescent probe for measurement of peroxyl radical scavenging activity in biological samples[J]. Journal of agricultural and food chemistry,2014,62(8):1839-1845
[39] 向言词,冯涛,刘炳荣,等.锰尾渣改良对4种植物吸收锰的影响[J].水土保持学报,2007,21(3):77-80
[40] 杨塍希,国伟强,和文懿,等.火炬树幼苗对镉胁迫的生理响应及积累特性[J].福建农林大学学报(自然科学版),2020,49(03):334-340
[41] SHABIR H WANI,VINAY KUMAR,VARSHA SHRIRAM,et al. Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants[J]. The Crop Journal,2016,4(03):162-176
[42] YONG Y E,YI L C,YOU H H,et al. Comparisons of tolerances to salt stress among three salt-secreting mangrove species[J]. Acta Ecologica Sinica,2004,43(11):171-196
[43] 铁梅,梁彦秋,张朝红,等. Cd污染地草坪草中Cd分布特征及化学形态的研究[J].应用生态学报,2002,13(2):175-178
[44] DIXIT,VIVEK,PANDEY,et al. Plants and the Environment. Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad)[J]. Journal of Experimental Botany,2001,52(358):1101-1101
[45] FRÉDÉRIC MEURIOT,NOQUET C,AVICE J C,et al. Methyl jasmonate alters N partitioning,N reserves accumulation and induces gene expression of a 32-kDa vegetative storage protein that possesses chitinase activity in Medicago sativa taproots[J]. Physiologia Plantarum,2004,120(1):113-123
[46] UEDA J,KATO J,YAMANE H,et al. Inhibitory effect of methyl jasmonate and its related compounds on kinetin-induced retardation of oat leaf senescence[J]. Physiologia Plantarum,2010,52(2):305-309
[47] 林植芳,李双顺,林桂珠,等.水稻叶片的衰老与超氧物歧化酶活性及脂质过氧化作用的关系[J]. Journal of Integrative Plant Biology,1984,26(6):47-57
[48] 冯晴,徐朗莱.小麦叶片衰老过程中CAT和APX活力及其同工酶谱的变化[J].南京农业大学学报,1997,20(002):95-99
[49] 吴汉福,田玲,翁贵英,等.煤矸石山优势植物对重金属吸收及富集特征[J].水土保持学报,2016,30(02):317-322
[50] C A GRANT,W T BUCKLEY,L D BAILEY,et al. Cadmium accumulation in crops[J]. Canadian Journal of Plant Science,1998,78(1):1-17
[51] 仇硕,张敏,孙延东,等.植物重金属镉(Cd²⁺)吸收、运输、积累及耐性机理研究进展[J].西北植物学报,2006,26(12):2615-2622
[52] 郭晋君,张沛,杏艳,等. pH对土壤吸附重金属镉的影响[J].广东化工,2013,40(08):116-117
[53] 王静,肖国举,毕江涛,等. pH对宁夏引黄灌区盐碱化土壤重金属吸附-解吸过程的影响[J].生态环境学报,2017,26(10):1782-1787
[54] 曾鹏,曹霞,郭朝晖,等.珊瑚树(Viburnum odoratissinum)对污染土壤中镉的耐受和富集特征[J].生态学报,2017,37(19):6472-6479
[55] 田如男,于双,王守攻.铜、镉胁迫下荻种子的萌发和幼苗生长[J].生态环境学报,2011,20(8-9):1332-1337

外源茉莉酸甲酯对镉胁迫下高羊茅生长及生理指标的影响

Effects of Exogenous Methyl Jasmonate on Growth and Physiological Indexes of Festuca elata under Cadmium Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杨迎月, 毛桂莲, 麻冬梅, 张峰举, 许兴. 四种牧草种子在不同浓度NaCl或NaHCO₃胁迫下的萌发特性[J]. 草地学报, 2022, 30(3): 637-645.
[2]	张杨杨, 李希铭, 高鹏, 宋桂龙. 不同浓度镉胁迫下6种草本植物的耐性及富集特征的比较[J]. 草地学报, 2021, 29(6): 1265-1276.
[3]	朱瑞婷, 牛奎举, 张娣君, 杨阳, 宋云, 马晖玲. 外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响[J]. 草地学报, 2021, 29(4): 635-643.
[4]	孔海明, 李开源, 高双红, 孙娈姿. 根瘤菌对紫花苜蓿生物修复镉污染功能的影响[J]. 草地学报, 2021, 29(12): 2763-2768.
[5]	李萱, 刘博文, 高双红, 游翔凯, 龙思, 李尤月, 刘茜, 张士军, 许岳飞. 外源硫化氢对不同弱光下高羊茅形态和生理指标的影响[J]. 草地学报, 2021, 29(11): 2435-2441.
[6]	高双红, 李媛英, 刘博文, 龙思, 刘铁芫, 许岳飞. H₂S对弱光胁迫下高羊茅幼苗生理特性和内源激素的影响[J]. 草地学报, 2021, 29(10): 2233-2239.
[7]	刘霄霏, 李惠英, 陈良, 徐倩, 胡龙兴, 王慧彬, 马向明. 外源硒对镉胁迫下黑麦草生长和生理的影响[J]. 草地学报, 2020, 28(1): 72-79.
[8]	鲜靖苹, 王勇, 马晖玲. 外源NO缓解草地早熟禾镉胁迫的转录组分析[J]. 草地学报, 2019, 27(6): 1467-1476.
[9]	鲜靖苹, 王勇, 马晖玲. 一氧化氮信号途径参与草地早熟禾耐镉机制的研究[J]. 草地学报, 2019, 27(6): 1577-1586.
[10]	邵将, 陈瑶, 刘大林, 陈鸣晖, 吴亚, 黄玉婷. 硅对镉胁迫下不同狼尾草属牧草生理代谢的影响[J]. 草地学报, 2018, 26(5): 1223-1228.
[11]	刘春阳, 李占台, 刘东霞, 王激清, 刘贵河. 速效氮肥和缓释肥配施对高羊茅草坪质量及土壤硝态氮动态的影响[J]. 草地学报, 2018, 26(5): 1248-1254.
[12]	见妮, 刘璐, 屈佳欣, 李凯, 王佺珍. 超声波对镉胁迫鸭茅和冰草种子萌发的影响[J]. 草地学报, 2018, 26(2): 427-434.
[13]	李昱彤, 齐实, 周金星, 郝文乾, 靳孟理, 胡译水. 城市废弃物堆肥的混配基质对高羊茅营养生长期的影响[J]. 草地学报, 2017, 25(5): 1069-1078.
[14]	马艳芳, 张旭虎, 孟雯雯, 柴睿, 祁彦文, 许岳飞. 外源一氧化氮及不同修剪高度对高羊茅根形态特征的影响[J]. 草地学报, 2017, 25(4): 777-781.
[15]	王宝媛, 濮阳雪华, 宋桂龙, 周述琼, 赵克奇, 蒋凯. 20个高羊茅品种镉耐性评价及富集特征[J]. 草地学报, 2017, 25(1): 107-114.