大叶山蚂蝗叶片响应缺磷胁迫的脂质代谢组分析

doi:10.11733/j.issn.1007-0435.2022.06.017

草地学报 ›› 2022, Vol. 30 ›› Issue (6): 1460-1467.DOI: 10.11733/j.issn.1007-0435.2022.06.017

• 研究论文 • 上一篇

大叶山蚂蝗叶片响应缺磷胁迫的脂质代谢组分析

郑韶爵^1,2, 朱厚荣^1,2, 罗佳佳², 刘攀道², 罗丽娟¹, 刘国道², 董荣书²

1. 海南大学热带作物学院, 海南海口 570228;
2. 中国热带农业科学院热带作物品种资源研究所, 海南海口 571101

收稿日期:2021-12-16 修回日期:2022-03-24 发布日期:2022-07-05
通讯作者: 刘国道,E-mail:dongrongshu@126.com;董荣书,E-mail:liuguodao2008@163.com
作者简介:郑韶爵(1997-),男,汉族,海南海口人,硕士研究生,主要从事草业科学研究,E-mail:578454842@qq.com
基金资助:
海南省科协青年科技英才学术创新计划项目（No.QCXM201901）；海南省重点研发计划科技合作方向项目（No.ZDYF2019194）；财政部和农业农村部国家现代农业产业技术体系（No.CARS-34/CARS-22）资助

Analysis of Lipid Metabolome Responding to Phosphorus Deficiency in the Desmodium gangeticum Leaves

ZHENG Shao-jue^1,2, ZHU Hou-rong^1,2, LUO Jia-jia², Liu Pan-dao², LUO Li-juan¹, LIU Guo-dao², DONG Rong-shu²

1. College of Tropical Crops, Hainan University, Haikou, Hainan Province 570228, China;
2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan Province 571101, China

Received:2021-12-16 Revised:2022-03-24 Published:2022-07-05

摘要/Abstract

摘要： 为分析大叶山蚂蝗对缺磷胁迫的响应，在水培条件下对其进行正常供磷（+P）和缺磷（-P）处理，并检测干重、磷含量及叶片脂质的变化。结果发现，-P处理10 d后，大叶山蚂蝗的地上部干重和磷含量显著降低。随后，对叶片的脂质组分析共鉴定到240个脂质，其中-P与+P处理间的差异脂质82个（上调43个，下调39个）。-P处理上调的脂质均不含磷，包括38个糖脂、3个甘油脂和2个固醇脂。而-P处理下调的脂质均为磷脂，包括14个磷脂酰乙醇胺、8个磷脂酰甘油，6个磷脂酰肌醇，5个磷脂酸，3个磷脂酰丝氨酸，1个磷脂酰胆碱，1个溶血磷脂酰胆碱，1个磷脂酰乙醇。本研究结果揭示了以不含磷的脂质替代磷脂是大叶山蚂蝗叶片适应缺磷胁迫的机制之一。

关键词: 大叶山蚂蝗, 缺磷胁迫, 脂质组学, 磷脂

Abstract: To analyze the response of Desmodium gangeticum to phosphorus deficiency stress,the plant was subjected to normal phosphorus supply (+P) and phosphorus deficiency (-P) treatments under hydroponic conditions and the changes of dry weight,phosphorus content and leaf lipids were determined. Results showed that the above-ground dry weight and phosphorus content of Desmodium gangeticum were significantly reduced after 10 days of -P treatment. Subsequently,lipidomic analysis of leaves identified a total of 240 lipids,including 82 differentially regulated lipids (43 up-regulated and 39 down-regulated) between the -P and +P treatments. The up-regulated lipids under -P treatment were all non-phosphorus ones,including 38 saccharolipids,3 glycerolipids and 2 sterolipids. In contrast,all lipids down-regulated by -P treatment were phospholipids,including 14 phosphatidylethano-lamine,8 phosphatidylglycerol,6 phosphatidylinositol,5 phosphatidic acid,3 phosphatidylserine,1 phosphatidylcholine,1 lyso-phosphatidylcholine,and 1 phosphatidylethanol. The results of this study reveal that the replacement of phospholipids by lipids that do not contain phosphorus is one of mechanisms,by which the leaves of Desmodium gangeticum adapt themselves to phosphorus deficiency stress.

Key words: Desmodium gangeticum, Phosphorus deficiency, Lipidomics, Phospholipids

中图分类号:

S541

郑韶爵, 朱厚荣, 罗佳佳, 刘攀道, 罗丽娟, 刘国道, 董荣书. 大叶山蚂蝗叶片响应缺磷胁迫的脂质代谢组分析[J]. 草地学报, 2022, 30(6): 1460-1467.

ZHENG Shao-jue, ZHU Hou-rong, LUO Jia-jia, Liu Pan-dao, LUO Li-juan, LIU Guo-dao, DONG Rong-shu. Analysis of Lipid Metabolome Responding to Phosphorus Deficiency in the Desmodium gangeticum Leaves[J]. Acta Agrestia Sinica, 2022, 30(6): 1460-1467.

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大叶山蚂蝗叶片响应缺磷胁迫的脂质代谢组分析

Analysis of Lipid Metabolome Responding to Phosphorus Deficiency in the Desmodium gangeticum Leaves

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