煤矸石山不同植物根际土壤AM真菌群落多样性分析

doi:10.11733/j.issn.1007-0435.2022.08.010

草地学报 ›› 2022, Vol. 30 ›› Issue (8): 2009-2018.DOI: 10.11733/j.issn.1007-0435.2022.08.010

• 研究论文 • 上一篇

煤矸石山不同植物根际土壤AM真菌群落多样性分析

甄莉娜, 刘丽珍, 牛艳, 李侠, 李朕, 吴娜, 王润梅

山西大同大学生命科学学院应用生物技术研究所, 山西大同 037009

收稿日期:2021-10-17 修回日期:2022-04-08 发布日期:2022-09-01
通讯作者: 王润梅,E-mail:wangrunmei2004@tom.com
作者简介:甄莉娜(1983-),女,山西洪洞人,博士,教授,主要从事矿区生态修复和草地土壤微生物研究,E-mail:zhenln2003@163.com
基金资助:
国家自然科学基金项目（31400479,31901227）；山西省应用基础研究计划项目（201801D121258，201901D111306）；大同市平城区重点研发计划项目（202003）；大同大学科研项目（2021CXZ7，202177）共同资助

Analysis of AM Fungi Community Diversity in Rhizosphere Soil of Different Vegetation in Coal Gangue Mountain

ZHEN Li-na, LIU Li-zhen, NIU Yan, LI Xia, LI Zhen, WU Na, WANG Run-mei

Applied Biotechnology Institute, College of Life Science, Shanxi Datong University, Datong, Shanxi Province 037009, China

Received:2021-10-17 Revised:2022-04-08 Published:2022-09-01

摘要/Abstract

摘要： 为探讨不同植物类型对煤矸石山丛枝菌根(Arbuscular mycorrhiza,AM)真菌群落的影响，本研究通过高通量测序的方法，调查了禾本科、菊科、豆科和藜科植物根际土壤的AM真菌群落结构及多样性，以期为煤矸石山生态修复中植物种类的选择提供理论依据。结果表明：4科植物共获得球囊菌门(Glomeromycota)OTU数454个，分属于5目7科7属 41种，禾本科和藜科植物的OTU数量较多。禾本科AM真菌α多样性中的Chao1指数和Shannon指数最高，藜科组成较为单一，豆科植物根际土壤菌群多样性较低。RDA分析表明，土壤有机质和pH值对AM真菌群落影响作用较大，不同植物AM真菌生态网络关系差异显著，禾本科和黎科植物网络连接密集程度更高且核心物种组成更丰富。研究认为：煤矸石山恢复初期，不同科植物根际AM真菌群落多样性不同，禾本科植物通过与不同种类AM真菌形成良好的共生关系，促使其在煤矸石山恢复早期占据优势地位。

关键词: 煤矸石, 丛枝菌根真菌, 多样性

Abstract: In order to explore the effect of different vegetation types on arbuscular mycorrhizal fungal community in coal gangue dump,the rhizosphere AMF community structure and diversity of Gramineae, Asteraceae,Leguminosae and Chenopodiaceae were investigated by high-throughput sequencing to provide a theoretical basis for plant species selection in coal gangue dump recovering process. The results showed that there were 454 OTUs of Glomeromycota in four plant families,which belonged to 5 orders,7 families,7 genera and 41 species. The number of OTUs of Gramineae and Chenopodiaceae were higher than others. According to the analysis of AMF community structure histogram,the composition of Chenopodiaceae was relatively simple,and the diversity of rhizosphere flora of leguminous plants was low at the genus level. Chao1 and shannon index of gramineous plants were the highest,and the similarity coefficient of AM fungal community in soil rhizosphere of gramineous species was higher than other plants. RDA analysis showed that soil organic matter and pH were the main influencing factors of AM fungal community. There were significant differences in the ecological network relationship of AM fungi in rhizosphere soil of different plants. Gramineae and Chenopodiaceae plants had higher network connection density and richer core species composition than the others. The results showed that the diversity of AM fungal communities in the rhizosphere of different plant families was different in the early stage of coal gangue recovery,and gramineous species plants formed a better symbiotic relationship with AM fungi in different recovery phase,which promoteed them to occupy a dominant position in the early recovery stage of coal gangue dump.

Key words: Coal gangue dump, Arbuscular mycorrhizal fungi, Diversity

中图分类号:

S154.3

甄莉娜, 刘丽珍, 牛艳, 李侠, 李朕, 吴娜, 王润梅. 煤矸石山不同植物根际土壤AM真菌群落多样性分析[J]. 草地学报, 2022, 30(8): 2009-2018.

ZHEN Li-na, LIU Li-zhen, NIU Yan, LI Xia, LI Zhen, WU Na, WANG Run-mei. Analysis of AM Fungi Community Diversity in Rhizosphere Soil of Different Vegetation in Coal Gangue Mountain[J]. Acta Agrestia Sinica, 2022, 30(8): 2009-2018.

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参考文献

[1] 李侠,任豪,贝水宽,等. 晋华宫矿煤矸石山植物群落分布特征及驱动因素[J]. 土壤通报,2018,49(4):813-821
[2] 唐骏,党廷辉,李俊超,等. 黄土区煤矿排土场重建草地土壤剖面有机碳变化特征[J]. 草地学报,2015(4):718-725
[3] 王化秋,程巍,郝俊,等. 贵州煤矸石山香根草根系及根际土丛枝菌根真菌(AMF)群落的季节动态研究[J]. 菌物学报,2021,40(3):514-530
[4] 石国玺,王芳萍,马丽,等. 长期,短期增温对高寒草甸AM真菌群落结构的影响[J]. 草地学报,2021,29(S1):11
[5] HUANG G M,SRIVASTAVA A K,ZOU Y N,et al. Exploring arbuscular mycorrhizal symbiosis in wetland plants with a focus on human impacts[J]. Symbiosis,2021,84(2):1-10
[6] 曹敏,胡开治,刘燕琴,等. 高通量测序分析重庆地区茅苍术根际丛枝菌根真菌多样性[J]. 微生物学通报,2020,47(9):2877-2886
[7] 王丽萍,张弘,钱奎梅,等. 丛枝菌根真菌对矿区修复系统固碳的作用[J]. 中国矿业大学学报,2012,41(4):635-640
[8] 王占军,马琨,崔慧珍,等. 土壤丛枝菌根真菌与宁夏主要草原类型植被群落分布间的相互关系研究[J]. 草业学报,2020,29(12):150-160
[9] CLAUDIA K,PETR K,MARTINA J,et al. Plant communities rather than soil properties structure arbuscular mycorrhizal fungal communities along primary succession on a mine spoil[J]. Frontiers in Microbiology,2017(8):719
[10] 李芳,高萍,段廷玉. AM菌根真菌对非生物逆境的响应及其机制[J]. 草地学报,2016,24(3):491-500
[11] EZEOKOLI O T,NWANGBURUKA C C,ADE R A,et al. Assessment of arbuscular mycorrhizal fungal spore density and viability in soil stockpiles of south african opencast coal mines[J]. South African Journal of Plant and Soil,2018,36(2):91-99
[12] WANG F Y,LIU R J,LIN X G,et al. Comparison of diversity of arbuscular mycorrhizal fungi in different ecological environments[J]. Acta Ecologica Sinica,2003,23(12):2666-2671
[13] ACDES M,BURITY H A,GOTO B T,et al. Diversity of arbuscular mycorrhizal fungi in a gypsum mining impacted semiarid area[J]. Acta Botanica Brasilica,2010,24(4):1052-1061
[14] 王志伟,纪燕玲,陈永敢,等. 禾本科植物内生真菌资源及其物种多样性[J]. 生态学报,2010,30(17):4771-4781
[15] LEUNG H M,YE Z H,WONG M H. Survival strategies of plants associated with arbuscular mycorrhizal fungi on toxic mine tailings[J]. Chemosphere,2007,66(5):905-915
[16] 孔令杰,柳旭,韩月龙,等. 黄顶菊与3种本地植物竞争对丛枝菌根真菌种类和多样性的影响[J]. 生物安全学报,2018,27(3):224-232
[17] 于宏清. AM真菌与盐碱地藜科植物的共生关系[D].长春:东北师范大学,2015:15-17
[18] 李华健,贺学礼. 河北峰峰矿区构树AM真菌物种多样性及生态适应性[J]. 河北大学学报(自然科学版),2019,39(3):278-287
[19] PHILLIPS J M. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection[J]. Transactions of the British Mycological Society,1970,55(1):157-160
[20] JIE W G,LIU X R,CAI B Y. Diversity of rhizosphere soil arbuscular mycorrhizal fungi in various soybean cultivars under different continuous cropping regimes.[J]. Plos one,2017,8(8):e72898
[21] 李凯荣,闫宝环,时亚坤. 黄土高原南缘煤矸石废弃地不同植物对重金属污染的耐受性研究[J].草地学报,2013,21(6):1093-1100
[22] 曹丽霞,福英,候伟峰,等. 丛枝菌根在退化生态系统恢复中的作用及其研究展望[J]. 北方园艺,2015(14):182-189
[23] 甄莉娜,李侠,李朕,等. 煤矸石山不同植被根际可培养微生物数量的动态变化[J]. 草地学报,2021,29(6):1224-1233
[24] 王锐,李希来,张静,等. 不同覆土处理对青海木里煤田排土场渣山表层土壤基质特征的影响[J]. 草地学报,2019,27(5):1266-1276
[25] NGUGI M R,FECHNER N,NELDNER V J,et al. Successional dynamics of soil fungal diversity along a restoration chronosequence post-coal mining[J]. Restoration Ecology,2020,28(3):543-552
[26] OZEOKOLI S,MASHIGO S,MABOETA M S,et al. Arbuscular mycorrhizal fungal community differentiation along a post-coal mining reclamation chronosequence in South Africa:A potential indicator of ecosystem recovery[J]. Applied Soil Ecology,2021(147):103429
[27] 吴佳伟,杨瑞,王勇,等. 贵州草海流域三种不同植被类型根际土壤真菌结构组成和多样性[J]. 菌物学报,2020,39(7):1250-1262
[28] VANDENKOORNHUYSE P,RIDGWAY K P,WATSON I J,et al. Co-existing grass species have distinctive arbuscular mycorrhizal communities[J]. Molecular Ecology,2010,12(11):3085-3095
[29] ZUBEK S,ROZEK K,STEFANWICZ A M,et al. The impact of beech and riparian forest herbaceous plant species with contrasting traits on arbuscular mycorrhizal fungi abundance and diversity[J]. Forest Ecology and Management,2021,492(3):119245
[30] BI Y L,XIE L L,WANG J,et al. Impact of host plants,slope position and subsidence on arbuscular mycorrhizal fungal communities in the coal mining area of north-central China[J]. Journal of Arid Environments,2018(163):68-76
[31] 张海波,梁月明,冯书珍,等. 土壤类型和树种对根际土丛枝菌根真菌群落及其根系侵染率的影响[J]. 农业现代化研究,2016,37(1):187-194
[32] ALGUACIL M M,TORRES M P,TORRECILLAS E,et al. Plant type differently promote the arbuscular mycorrhizal fungi biodiversity in the rhizosphere after revegetation of a degraded,semiarid land[J]. Soil Biology & Biochemistry,2011,43(1):167-173
[33] WANG,J,WANG G G,ZHANG B,et al. Arbuscular mycorrhizal fungi associated with tree species in a planted forest of Eastern China[J]. Forests,2019(10):424
[34] 牛天心. 补连塔煤矿采陷区丛枝菌根真菌种类分布及土壤因子的研究[D]. 呼和浩特:内蒙古大学,2015:28-30
[35] ALLEN E B,ALLEN M F,EGERTON W L,et al. Impacts of early and late seral mycorrhizal during restoration in seasonal tropical forest,Mexico[J]. Ecological Applications,2003,13(6):1701-1710
[36] 张哲超,杨久扬,郝百惠,等. 微生物群落驱动AM真菌,生物炭及联合改良沙化土壤作用潜力[J]. 环境科学,2021,42(4):2066-2079
[37] ZHANG Z,SHI Z,YANG J,et al. A new strategy for evaluating the improvement effectiveness of degraded soil based on the synergy and diversity of microbial ecological function[J]. Ecological Indicators,2021,120(2):106917

煤矸石山不同植物根际土壤AM真菌群落多样性分析

Analysis of AM Fungi Community Diversity in Rhizosphere Soil of Different Vegetation in Coal Gangue Mountain

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