不同氮化合物添加对晋北赖草草地CH4通量的影响

doi:10.11733/j.issn.1007-0435.2023.07.025

草地学报 ›› 2023, Vol. 31 ›› Issue (7): 2146-2154.DOI: 10.11733/j.issn.1007-0435.2023.07.025

不同氮化合物添加对晋北赖草草地CH₄通量的影响

卢英帅^1,2,3, 彭洁^1,2,3, 李彦颉^1,2,3, 燕学东^1,2,3, 郝杰^1,2,3, 刁华杰^1,2,3, 苏原^1,2,3, 徐沛东^1,2,3, 董宽虎^1,2,3, 王常慧^1,2,3, 陈晓鹏^1,2,3

1. 山西农业大学草业学院, 山西太谷 030801;
2. 草地生态保护与乡土草种质创新山西省重点实验室, 山西太谷 030801;
3. 山西右玉黄土高原草地生态系统国家定位观测研究站, 山西右玉 037200

收稿日期:2022-12-08 修回日期:2023-03-06 出版日期:2023-07-15 发布日期:2023-08-01
通讯作者: 陈晓鹏,E-mail:chenxp@sxau.edu.cn
作者简介:卢英帅(1997-),男,汉族,河南滑县人,硕士研究生,主要从事草地生态与管理研究,E-mail:luysmomd@163.com
基金资助:
国家自然科学基金项目(32271682)；山西省科技厅平台专项(202104010910017)；山西农业大学高层次引进人才专项(2021XG008)资助

Effect of Different Nitrogen Compounds Addition on CH₄ Flux of Leymus secalinus Grassland in Northern Shanxi Province

LU Ying-shuai^1,2,3, PENG Jie^1,2,3, LI Yan-jie^1,2,3, YAN Xue-dong^1,2,3, HAO Jie^1,2,3, DIAO Hua-jie^1,2,3, SU Yuan^1,2,3, XU Pei-dong^1,2,3, DONG Kuan-hu^1,2,3, WANG Chang-hui^1,2,3, CHEN Xiao-peng^1,2,3

1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. Shanxi Key Laboratory of Grassland Ecological Protection and Native Grass Germplasm Innovation, Taigu, Shanxi Province 030801, China;
3. Youyu Loess Plateau Grassland Ecosystem National Research Station, Youyu, Shanxi Province 03720, China

Received:2022-12-08 Revised:2023-03-06 Online:2023-07-15 Published:2023-08-01

摘要/Abstract

摘要： 土壤中氮的存在形式是影响CH₄通量的因子之一。为阐明不同氮化合物输入对草地生态系统CH₄通量的影响及其机制，本研究以晋北赖草草地为研究对象，设置对照、硝酸铵、硫酸铵、碳酸氢铵、尿素和缓释尿素添加6个处理，采用静态箱-气相色谱法对2021年生长季草地CH₄通量及其潜在影响因子进行测定，结果表明：晋北赖草草地在生长季是大气CH₄的弱汇，生长季对照处理平均CH₄吸收速率为5.35 μg·m^-2·h^-1；不同氮化合物添加对CH₄吸收速率无显著影响且并未改变CH₄通量的季节波动趋势；无机态与有机态氮添加处理间CH₄吸收速率无显著差异；土壤硝态氮含量和微生物生物量碳氮比是CH₄吸收的主要影响因子；路径分析发现硝态氮含量增加抑制CH₄吸收，表明调控晋北赖草草地CH₄吸收的是土壤氮的有效性而非氮源的化学组成。

关键词: 氮添加, 温室气体, 氮形态, 甲烷吸收

Abstract: CH₄ fluxes can be affected by the form of soil nitrogen. To clarify the effect of different nitrogen compounds input on CH₄ fluxes and its influencing mechanism,we installed six treatments as the control,ammonium nitrate addition,ammonium sulfate addition,ammonium bicarbonate addition,urea addition and slow-release urea addition into a grassland community dominated by Leymus secalinus in northern Shanxi Province. The CH₄ fluxes were measured by static chamber-gas chromatography method and its potential influencing factors were also investigated during the growing season of 2021. The results showed that the Leymus secalinus grassland was a weak CH₄ sink during the growth season,and the average CH₄ uptake rate of control was 5.35 μg·m^-2·h^-1. Different nitrogen compounds additions had no significant effect on CH₄ fluxes,and did not change seasonal fluctuation pattern of CH₄ fluxes. There was no significant difference in CH₄ uptake rate between inorganic and organic nitrogen addition. Soil CH₄ fluxes were mainly determined by soil NO^-₃-N and carbon：nitrogen ratio of microbial biomass. Path analysis showed that the increase of NO^-₃-N content inhibited CH₄ uptake,indicating that it was the availability of soil nitrogen that regulated CH₄ uptake in Leymus Secalinus grassland in northern Shanxi,rather than the chemical composition of nitrogen sources.

Key words: Nitrogen addition, Greenhouse gas, Nitrogen forms, Methane uptake

中图分类号:

S812.2

卢英帅, 彭洁, 李彦颉, 燕学东, 郝杰, 刁华杰, 苏原, 徐沛东, 董宽虎, 王常慧, 陈晓鹏. 不同氮化合物添加对晋北赖草草地CH₄通量的影响[J]. 草地学报, 2023, 31(7): 2146-2154.

LU Ying-shuai, PENG Jie, LI Yan-jie, YAN Xue-dong, HAO Jie, DIAO Hua-jie, SU Yuan, XU Pei-dong, DONG Kuan-hu, WANG Chang-hui, CHEN Xiao-peng. Effect of Different Nitrogen Compounds Addition on CH₄ Flux of Leymus secalinus Grassland in Northern Shanxi Province[J]. Acta Agrestia Sinica, 2023, 31(7): 2146-2154.

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参考文献

[1] PACHAURI R K,ALLEN M R,BARROS V R,et al. Contribution of Working Groups I, II and III to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change, Climate Change 2014:Synthesis Report[R]. Geneva,Switzerland:IPCC,2014:87
[2] LUANA B,ANDY C,HAN D,et al. The State of Greenhouse Gases in the Atmosphere Based on Global Observations through 2020[R]. Switzerland:WMO Greenhouse Gas Bulletin,2022:7
[3] YU L,HUANG Y,ZHANG W,et al. Methane uptake in global forest and grassland soils from 1981 to 2010[J]. Science of the Total Environment,2017,607-608:1163-1172
[4] FANG H J,CHENG S L,YU G R,et al. Low-level nitrogen deposition significantly inhibits methane uptake from an alpine meadow soil on the Qinghai-Tibetan Plateau[J]. Geoderma,2014,213:444-452
[5] 高鑫华,朱婷,邹斌等. 氮源类型对甲烷氧化过程主要菌群的影响[J]. 基因组学与应用生物学,2017,36(3):966-975
[6] YU G R,JIA Y L,HE N P,et al. Stabilization of atmospheric nitrogen deposition in China over the past decade[J]. Nature Geoscience,2019,12(6):424-429
[7] ZHANG Y,SONG L,LIU X J. Atmospheric organic nitrogen deposition in China[J]. Atmospheric Environment,2012,46:194-204
[8] GOMEZ-CASANOVAS N,HUDIBURG T,BERNACCHI C,et al. Nitrogen deposition and greenhouse gas emissions from grasslands:uncertainties and future directions[J]. Global Change Biology,2016,22(4):1348-1360
[9] ZHAO Z,DONG S,JIANG X,et al. Effects of warming and nitrogen deposition on CH₄,CO₂ and N₂O emissions in alpine grassland ecosystems of the Qinghai-Tibetan Plateau[J]. Science of the Total Environment,2017,592:565-572
[10] PENG Y F,WANG G Q,LI F,et al. Unimodal Response of Soil Methane Consumption to Increasing Nitrogen Additions[J]. Environmental Science and Technology,2019,53(8):4150-4160
[11] DONG L L,SUN T,BERG B,et al. Effects of different forms of N deposition on leaf litter decomposition and extracellular enzyme activities in a temperate grassland[J]. Soil Biology and Biochemistry,2019,134:78-80
[12] CHEN J H,ZHANG Y J,YANG Y,et al. Effects of increasing organic nitrogen inputs on CO₂,CH₄,and N₂O fluxes in a temperate grassland[J]. Environmental Pollution,2021,268:115822
[13] LI X Y,CHENG S L,FANG H J,et al. The contrasting effects of deposited NH⁺₄ and NO^-₃ on soil CO₂,CH₄ and N₂O fluxes in a subtropical plantation,southern China[J]. Ecological Engineering,2015,85:317-327
[14] INSELSBACHER E,WANEK W,RIPKA K,et al. Greenhouse gas fluxes respond to different N fertilizer types due to altered plant-soil-microbe interactions[J]. Plant and Soil,2011,343(1):17-35
[15] WANG Y S,CHENG S L,FANG H J,et al. Simulated nitrogen deposition reduces CH₄ uptake and increases N₂O emission from a subtropical plantation forest soil in southern China[J]. PloS One,2014,9(4):e93571
[16] YANG X T,WANG C M,XU K. Response of soil CH₄ fluxes to stimulated nitrogen deposition in a temperate deciduous forest in northern China:A 5-year nitrogen addition experiment[J]. European Journal of Soil Biology,2017,82:43-49
[17] KING G M,SCHNELL S. Effects of ammonium and non-ammonium salt additions on methane oxidation by Methylosinus trichosporium OB3b and maine forest soils[J]. Applied and Environmental Microbiology,1998,64(1):253-257
[18] 郑慧,薛江博,郝杰,等. 短期不同水平氮添加对华北盐渍化草地土壤磷组分的影响[J]. 草地学报,2022,30(3):712-720
[19] 赵芳草,陈鸿飞,王一昊,等. 盐渍化草地根际土壤理化性质对降水改变和氮添加的响应[J]. 草地学报,2022,30(9):2430-2437
[20] 刘平,刘学军,骆晓声等. 山西北部农村区域大气活性氮沉降特征[J]. 生态学报,2016,36(17):5353-5359
[21] ZHANG D Y,PENG Y F,LI F,et al. Trait identity and functional diversity co-drive response of ecosystem productivity to nitrogen enrichment[J]. Journal of Ecology,2019,107(5):2402-2414
[22] 王立刚. 农业源温室气体监测技术规程与控制技术研究[M]. 北京:科学出版社,2016:46
[23] 秦加敏,王常慧,曹颖,等. 氮添加和刈割对内蒙古典型草原植被碳氮库的影响[J]. 草地学报,2022,30(1):12-20
[24] BROOKES P C,KRAGT J F,POWLSON D S,et al. Chloroform fumigation and the release of soil nitrogen:The effects of fumigation time and temperature[J]. Soil Biology and Biochemistry,1985,17(6):831-835
[25] VANCE E D,BROOKES P C,JENKINSON D S. An extraction method for measuring soil microbial biomass C[J]. Soil Biology and Biochemistry,1987,19(6):703-707
[26] REN R,XU W,ZHAO M,et al. Grazing offsets the stimulating effects of nitrogen addition on soil CH₄ emissions in a meadow steppe in Northeast China[J]. PloS One,2019,14(12):e0225862
[27] LI X,HE H,YUAN W,et al. Response of soil methane uptake to simulated nitrogen deposition and grazing management across three types of steppe in Inner Mongolia,China[J]. Science of the Total Environment,2018,612:799-808
[28] DIAO H J,CHEN X P,WANG G,et al. The response of soil respiration to different N compounds addition in a saline-alkaline grassland of northern China[J]. Journal of Plant Ecology,2022,15(5):897-910
[29] REDDY K R,RAI R K,Green S J,et al. Effect of pH on methane oxidation and community composition in landfill cover soil[J]. Journal of Environmental Engineering,2020,146(6):04020037
[30] GAO J B,ZHOU W J,LIU Y T,et al. Litter-derived nitrogen reduces methane uptake in tropical rainforest soils[J]. Science of the Total Environment,2022,849:157891
[31] SAARI A,RINNAN R,MARTIKAINEN P J. Methane oxidation in boreal forest soils:kinetics and sensitivity to pH and ammonium[J]. Soil Biology and Biochemistry,2004,36(7):1037-1046
[32] LU X K,MAO Q G,GILLIAM F S. Nitrogen deposition contributes to soil acidification in tropical ecosystems[J]. Global Change Biology,2014,20(12):3790-3801
[33] ZHOU J,XIA F,LIU X G,et al. Effects of nitrogen fertilizer on the acidification of two typical acid soils in South China[J]. Journal of Soils and Sediments,2014,14(2):415-422
[34] CAI J P,LUO W T,LIU H Y,et al. Precipitation-mediated responses of soil acid buffering capacity to long-term nitrogen addition in a semi-arid grassland[J]. Atmospheric Environment,2017,170:312-318
[35] BODELIER P L E,LAANBROEK H J. Nitrogen as a regulatory factor of methane oxidation in soils and sediments[J]. FEMS Microbiology Ecology,2004,47(3):265-277
[36] 杨山,李小彬,王汝振等. 氮水添加对中国北方草原土壤细菌多样性和群落结构的影响[J]. 应用生态学报,2015,26(3):739-746
[37] LI K H,Gong Y M,SONG W,et al. Responses of CH₄,CO₂ and N₂O fluxes to increasing nitrogen deposition in alpine grassland of the Tianshan Mountains[J]. Chemosphere:Environmental Toxicology and Risk Assessment,2012,88(1):140-143
[38] JIANG C M,YU G R,FANG H J,et al. Short-term effect of increasing nitrogen deposition on CO₂,CH₄ and N₂O fluxes in an alpine meadow on the Qinghai-Tibetan Plateau,China[J]. Atmospheric Environment,2010,44(24):2920-2926
[39] ZHANG L,HUO Y,GUO D,et al. Effects of multi-nutrient additions on GHG fluxes in a temperate grassland of Northern China[J]. Ecosystems,2014,17:657-672
[40] 郭家宏,范熠,张西美. 温度对不同生态系统土壤甲烷氧化过程和甲烷氧化细菌的影响[J]. 中国农业气象,2022,43(6):427-439
[41] 潘红,李勇,孟春梅,等. 氮素水平对土壤甲烷氧化和硝化微生物相互作用的影响[J]. 土壤学报,2022,59(2):557-567
[42] BODELIER P L E. Interactions between nitrogenous fertilizers and methane cycling in wetland and upland soils[J]. Current Opinion in Environmental Sustainability,2011,3(5):379-388
[43] LEGAY N,GRASSEIN F,ARNOLDI C,et al. Studies of NH⁺₄ and NO^-₃ uptake ability of subalpine plants and resource-use strategy identified by their functional traits[J]. Oikos,2020,129(6):830-841
[44] DAVIDSON E A. Soil water content and the ratio of nitrous oxide to nitric oxide emitted from soil[J]. Biogeochemistry of Global Change, 1993,5:369-386

不同氮化合物添加对晋北赖草草地CH₄通量的影响

Effect of Different Nitrogen Compounds Addition on CH₄ Flux of Leymus secalinus Grassland in Northern Shanxi Province

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