草地学报

›› 1994, Vol. 2 ›› Issue (1): 36-39.DOI: 10.11733/j.issn.1007-0435.1994.01.006

• 研究论文 • 上一篇    下一篇

超低温(LN2,-196℃)贮存牧草种子初探

孙丽萍, 宁怖, 包来晓, 李临杭   

  1. 中国农业科学院草原研究所, 呼和浩特, 010010
  • 出版日期:1994-03-15 发布日期:2012-07-11

GRYOPRESERVATION OF FORAGE SEED GERMPLASM

Sun Liping, Ning Bu, Bao Laixiao, Li Linhang   

  1. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Huhhot, 010010
  • Online:1994-03-15 Published:2012-07-11

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摘要： 在牧草种子的贮存过程中,最重要且最难于解决的问题就是克服所贮存种子的生理衰变。超低温技术(LN2,于－196℃条件)可以将种子内部新陈代谢降低到一个很低的水平,使所有的生化过程处于“停顿”状态,这样便可以大大延长种子的贮存寿命。本实验将9种在常规条件下难于保持活力的牧草种子放入液氮中贮存一年,解冻后测定贮存种子的发芽率、发芽指数、电导率、呼吸强度及ATP含量情况。结果表明,禾本科、豆科、藜科牧草种子属于液氮耐受类型,特别是短命种子驼绒藜与木地肤对液氮贮存表现特别适应。

关键词: 超低温技术, 贮存寿命, 种子活力

Abstract: Overcoming physilogical deterioration of a stored seed sample is one of the most important and difficult tasks in the preservation process.Cryopreservation technique (LN2, at -196℃)provides a po tential preservation by reducing metabolism to a low level at that all biochemical process are significantly reduced and biological decterioration virtually stopped.Thus,the seed storage life was greatly extended.Nine kind forage seed samples were stored in LN2 for one year.Then viability,conductivity,respiration and the content of ATP of the seed were measured to determine its vigor.Grass family, legume family and goose foot family were the type of LN2-tolerant.Especially, Ceratoid et arborescens (Losinsk) Tsien et C.C.Ma and Kochia rpstrata(L.)Schrad,were significant suitable for cryopreservation.

Key words: Cryopreservation technique, Storage life, Seed vigor