加速老化对高羊茅种子生理生化特性的影响

doi:10.11733/j.issn.1007-0435.1998.03.006

›› 1998, Vol. 6 ›› Issue (3): 191-196.DOI: 10.11733/j.issn.1007-0435.1998.03.006

加速老化对高羊茅种子生理生化特性的影响

浦心春, 韩建国, 毛培胜, 王培

中国农业大学草地研究所, 北京, 100094

出版日期:1998-08-15 发布日期:1998-08-15

Effects of Accelerated Ageing on the Physiological and Biochemical Characters of Tall Fescue Seed

Pu Xinchun, Han Jianguo, Mao Peisheng, Wang Pei

Institute of Grassland science, China Agricultural University, Bejing 100094

Online:1998-08-15 Published:1998-08-15

摘要/Abstract

摘要： 本研究对未老化与加速老化处理的高羊茅种子进行发芽率、浸出滚电导率、葡萄糖含量、超氧化物歧化酶(SOD)同工酶、发芽过程中ATP含量的测定结果表明,高羊茅种子随着老化时间的增加,发芽率和发芽势明显下降,而发芽势下降速度远大于发芽率。老化种子浸出液的电导率明显高于对照,并随着老化时间的延长,浸出液的电导率逐渐增加,浸出液中葡萄糖含量显著高于对照。轻微老化可刺激高羊茅种子中超氧化物歧化酶(SOD)同工酶出现新的酶带,防止膜系统受到破坏。与对照相比,老化种子在发芽过程中ATP含量均明显地降低,严重老化种子在发芽前期因ATP利用受到限制,ATP含量较高,但随着发芽的延续含量则大幅度降低。

关键词: 高羊茅种子, 人工加速老化, 生理生化变化

Abstract: Experiments were conducted to determine the germination rate, electrical conductivity,glucose content, SOD isoenzyme and ATP content of seed leachates during seed germination in ageing ornon-ageing tall fescue seed.The results showed that germination rate and germination speed decreasedwith the increasing of accelerated ageing time in tall fescue seed, and germination speed decreased fasterthan germination rate, the electrical conductivity and glucose content of seed leachates in ageing seed werehigher than those in non-ageing seed, and electrical conductivity gradually increaed with the extendingageing time.New pattems of SOD isoenzyme, which formed by stimulation of lightly accelerated ageing,could prevent the damage of membrane system.ATP content of ageing seed obviously decreased duringgermination contrasting with non-ageing seed.ATP content was high in seriously ageing seed during earlygermination due to the ATP utilization was limited, but ATP content decreased rapidly with the prolong ofthe germination

Key words: Tall fescue seed, Accelerated ageing, Physiological and biochemical change

浦心春, 韩建国, 毛培胜, 王培. 加速老化对高羊茅种子生理生化特性的影响[J]. , 1998, 6(3): 191-196.

Pu Xinchun, Han Jianguo, Mao Peisheng, Wang Pei. Effects of Accelerated Ageing on the Physiological and Biochemical Characters of Tall Fescue Seed[J]. , 1998, 6(3): 191-196.

参考文献

[1] 吕凤金、马缘生,1992.人工老化黑麦、小麦种子及其遗传完整性.全国首届青年农学学术年会论文集,北京:中国科技出版社,156-159.
[2] 黄上志、傅家瑞,1987.杂交水稻和不育种子的劣变与生理生化变化.植物生理学报,13(2):229-235.
[3] 徐本美、顾增辉,1984.萌动过程中种子ATP含量的变化.植物生理通迅,(3):39-44.
[4] 韩建国,1997.实用牧草种子学.北京:中国农业大学出版社,113-155.
[5] 韩建国、浦心春、毛培胜,1998.高羊茅种子老化过程中酶活性变化.草地学报,6(2):84-89.
[6] Abdul-Baki A A,1969.Relationship of glucose metabolism to germinability and vigor in barley and wheat seeds.Crop Science,9:732-737.
[7] Abdul-Baki A A, Anderson J D,1970.Viability and leaching of sugars from gemunating barley seed.Crop Science, I0:31-34.
[8] Basavarajappa B S, Shetty H S, Prakash, H S,1991.Membrane maize seed.Seed Science and Technology, 19(2):279-286.
[9] Ching T M, 1973.Biochemical aspects of seed vigor.Seed Science and Technology, 1:73-88.
[10] Ching T M,Schoolcraft I,1968.Physiological and chemical differences in aged seeds.Crop Science,8:407-409.
[11] Hampton J G,Tekrony D M,1995.ISTA handbook of vigour test methods.3rd Edition,Zurich,l-117.
[12] Heydecker W, 1972.Gemrination of an idea:the priming of seeds.University of Nottingham School of Agriculture, Annual Report,50-67.
[13] ISTA,1993.International rules for seed testing.Seed Sci&Technol.2l:Supplement Rules, l-260.
[14] Normah M N,Chin H F,1991.Changes in germination,respiration rate and leachate conductivity during storage of hevea seeds.Pertanika,l4(1):1-6.
[15] Rao K V M, Kalpana,1994.Carbohydrates and the ageing process in seeds of pigeonpea(Cajanus canan(L.)Millsp.)cultivars.Seed Science and Technology, 22(3):495-501.
[16] Woodstock L W,1973.Physiological and biochemical tests for seed vigor.Seed Science and Technology, l:I27-157

加速老化对高羊茅种子生理生化特性的影响

Effects of Accelerated Ageing on the Physiological and Biochemical Characters of Tall Fescue Seed

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价

[1]	马向丽, 何超, 罗富成, 许文花, 邓菊芬, 韩博, 段新慧, 张桓溥. 人工老化对纳罗克非洲狗尾草种子活力、生理生化指标的影响[J]. 草地学报, 2017, 25(5): 1047-1054.
[2]	李玉荣, 韩建国, 孙彦, 任卫波, 王小山. 新麦草种子劣变过程中生理生化变化[J]. , 2005, 13(3): 180-183,189.
[3]	韩建国, 浦心春, 毛培胜. 高羊茅种子老化过程中酶活性的变化[J]. , 1998, 6(2): 84-89.
[4]	浦心春, 韩建国, 王培, 毛培胜. 高羊茅种子生活力丧失过程中遗传物质的降解[J]. , 1996, 4(3): 180-185.